Catalytic module of human CTLH E3 ligase bound to multiphosphorylated UBE2H~ubiquitin
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8pjn
Q-score: 0.529
Chrustowicz J, Sherpa D, Li J, Langlois CR, Papadopoulou EC, Vu DT, Hehl LA, Karayel O, Beier V, von Gronau S, Muller J, Prabu JR, Mann M, Kleiger G, Alpi AF, Schulman BA
Mol Cell (2024) 84 pp. 293- [ DOI: doi:10.1016/j.molcel.2023.11.027 Pubmed: 38113892 ]
- Complex of human ctlhsr4 e3 ligase bound to engineered armc8-specific vh and multiphosphorylated ube2h~ubiquitin (600 kDa, Complex from Homo sapiens)
- Ubiquitin-conjugating enzyme e2 h (21 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein transferase maea (45 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein transferase rmnd5a (44 kDa, Protein from Homo sapiens)
Catalytic module of yeast GID E3 ligase bound to multiphosphorylated Ubc8~ubiquitin
Resolution: 3.53 Å
EM Method: Single-particle
Fitted PDBs: 8pmq
Q-score: 0.514
Chrustowicz J, Sherpa D, Li J, Langlois CR, Papadopoulou EC, Vu DT, Hehl LA, Karayel O, Beier V, von Gronau S, Muller J, Prabu JR, Mann M, Kleiger G, Alpi AF, Schulman BA
Mol Cell (2024) 84 pp. 293- [ DOI: doi:10.1016/j.molcel.2023.11.027 Pubmed: 38113892 ]
- Protein fyv10 (59 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein ligase rmd5 (49 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-conjugating enzyme e2-24 kda (24 kDa, Protein from Saccharomyces cerevisiae)
- Complex of yeast chelator-gidsr4 e3 ligase, tetrameric fbp1 substrate and multiphosphorylated ubc8~ubiquitin (1 MDa, Complex from Saccharomyces cerevisiae)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7ns3
Q-score: 0.52
Sherpa D, Chrustowicz J, Qiao S, Langlois CR, Hehl LA, Gottemukkala KV, Hansen FM, Karayel O, von Gronau S, Prabu JR, Mann M, Alpi AF, Schulman BA
Mol Cell (2021) 81 pp. 2445- [ Pubmed: 33905682 DOI: doi:10.1016/j.molcel.2021.03.025 ]
- Glucose-induced degradation protein 8 (55 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Protein fyv10 (59 kDa, Protein from Saccharomyces cerevisiae)
- Vacuolar import and degradation protein 24 (41 kDa, Protein from Saccharomyces cerevisiae)
- Fructose-bisphosphatase (38 kDa, Protein from Saccharomyces cerevisiae)
- Bj4_g0018240.mrna.1.cds.1 (108 kDa, Protein from Saccharomyces cerevisiae)
- Substrate receptor scaffolding module of yeast chelator-gid sr4 comprising gid1, gid4, gid5, gid8 and gid9 bound to fbp1 (310 kDa, Complex from Saccharomyces cerevisiae)
- Vacuolar import and degradation protein 28 (105 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Catalytic module of yeast Chelator-GID SR4 E3 ubiquitin ligase
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7ns4
Q-score: 0.498
Sherpa D, Chrustowicz J, Qiao S, Langlois CR, Hehl LA, Gottemukkala KV, Hansen FM, Karayel O, von Gronau S, Prabu JR, Mann M, Alpi AF, Schulman BA
Mol Cell (2021) 81 pp. 2445- [ Pubmed: 33905682 DOI: doi:10.1016/j.molcel.2021.03.025 ]
- Catalytic module of yeast chelator-gid sr4 comprising gid2 and gid9 (110 kDa, Complex from Saccharomyces cerevisiae)
- Protein fyv10 (59 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- E3 ubiquitin-protein ligase rmd5 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
Supramolecular assembly module of yeast Chelator-GID SR4 E3 ubiquitin ligase
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7nsb
Q-score: 0.5
Sherpa D, Chrustowicz J, Qiao S, Langlois CR, Hehl LA, Gottemukkala KV, Hansen FM, Karayel O, von Gronau S, Prabu JR, Mann M, Alpi AF, Schulman BA
Mol Cell (2021) 81 pp. 2445- [ Pubmed: 33905682 DOI: doi:10.1016/j.molcel.2021.03.025 ]
- Glucose-induced degradation protein 8 (55 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Glucose-induced degradation protein 7 (84 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Vacuolar import and degradation protein 30 (108 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Supramolecular assembly module of yeast chelator-gid sr4 comprising gid1, gid8 and a homodimer of gid7 (330 kDa, Complex from Saccharomyces cerevisiae)
Substrate receptor scaffolding module of human CTLH E3 ubiquitin ligase
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7nsc
Q-score: 0.515
Sherpa D, Chrustowicz J, Qiao S, Langlois CR, Hehl LA, Gottemukkala KV, Hansen FM, Karayel O, von Gronau S, Prabu JR, Mann M, Alpi AF, Schulman BA
Mol Cell (2021) 81 pp. 2445- [ Pubmed: 33905682 DOI: doi:10.1016/j.molcel.2021.03.025 ]
- Ran-binding protein 9 (77 kDa, Protein from Homo sapiens)
- Glucose-induced degradation protein 8 homolog (30 kDa, Protein from Homo sapiens)
- Isoform 2 of armadillo repeat-containing protein 8 (74 kDa, Protein from Homo sapiens)
- Glucose-induced degradation protein 4 homolog (33 kDa, Protein from Homo sapiens)
- Srs module of human ctlh complex comprising ranbp9, twa1, armc8 and hgid4 (210 kDa, Complex from Homo sapiens)