Structure of the non-canonical CTLH E3 substrate receptor WDR26 bound to YPEL5
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8qbn
Q-score: 0.581
Gottemukkala KV, Chrustowicz J, Sherpa D, Sepic S, Vu DT, Karayel O, Papadopoulou EC, Gross A, Schorpp K, von Gronau S, Hadian K, Murray PJ, Mann M, Schulman BA, Alpi AF
Mol Cell (2024) 84 pp. 1948-1963.e11 [ DOI: doi:10.1016/j.molcel.2024.04.014 Pubmed: 38759627 ]
- Complex of human wdr26-ctlh e3 ligase bound to ypel5 (900 kDa, Complex from Homo sapiens)
- Wd repeat-containing protein 26 (70 kDa, Protein from Homo sapiens)
- Protein yippee-like 5 (13 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
Structure of the non-canonical CTLH E3 substrate receptor WDR26 bound to NMNAT1 substrate
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8qe8
Q-score: 0.53
Gottemukkala KV, Chrustowicz J, Sherpa D, Sepic S, Vu DT, Karayel O, Papadopoulou EC, Gross A, Schorpp K, von Gronau S, Hadian K, Murray PJ, Mann M, Schulman BA, Alpi AF
Mol Cell (2024) 84 pp. 1948-1963.e11 [ DOI: doi:10.1016/j.molcel.2024.04.014 Pubmed: 38759627 ]
- Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1 (31 kDa, Protein from Homo sapiens)
- Beta-nicotinamide ribose monophosphate (335 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Wd repeat-containing protein 26 (70 kDa, Protein from Homo sapiens)
- Complex of human wdr26-ctlh e3 ligase bound to nmnat1 (1 MDa, Complex from Homo sapiens)
Catalytic module of human CTLH E3 ligase bound to multiphosphorylated UBE2H~ubiquitin
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8pjn
Q-score: 0.529
Chrustowicz J, Sherpa D, Li J, Langlois CR, Papadopoulou EC, Vu DT, Hehl LA, Karayel O, Beier V, von Gronau S, Muller J, Prabu JR, Mann M, Kleiger G, Alpi AF, Schulman BA
Mol Cell (2024) 84 pp. 293- [ DOI: doi:10.1016/j.molcel.2023.11.027 Pubmed: 38113892 ]
- Complex of human ctlhsr4 e3 ligase bound to engineered armc8-specific vh and multiphosphorylated ube2h~ubiquitin (600 kDa, Complex from Homo sapiens)
- Ubiquitin-conjugating enzyme e2 h (21 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein transferase maea (45 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein transferase rmnd5a (44 kDa, Protein from Homo sapiens)
Catalytic module of yeast GID E3 ligase bound to multiphosphorylated Ubc8~ubiquitin
Resolution: 3.53 Å
EM Method: Single-particle
Fitted PDBs: 8pmq
Q-score: 0.514
Chrustowicz J, Sherpa D, Li J, Langlois CR, Papadopoulou EC, Vu DT, Hehl LA, Karayel O, Beier V, von Gronau S, Muller J, Prabu JR, Mann M, Kleiger G, Alpi AF, Schulman BA
Mol Cell (2024) 84 pp. 293- [ DOI: doi:10.1016/j.molcel.2023.11.027 Pubmed: 38113892 ]
- Protein fyv10 (59 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein ligase rmd5 (49 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-conjugating enzyme e2-24 kda (24 kDa, Protein from Saccharomyces cerevisiae)
- Complex of yeast chelator-gidsr4 e3 ligase, tetrameric fbp1 substrate and multiphosphorylated ubc8~ubiquitin (1 MDa, Complex from Saccharomyces cerevisiae)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7ns3
Q-score: 0.52
Sherpa D, Chrustowicz J, Qiao S, Langlois CR, Hehl LA, Gottemukkala KV, Hansen FM, Karayel O, von Gronau S, Prabu JR, Mann M, Alpi AF, Schulman BA
Mol Cell (2021) 81 pp. 2445- [ Pubmed: 33905682 DOI: doi:10.1016/j.molcel.2021.03.025 ]
- Glucose-induced degradation protein 8 (55 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Protein fyv10 (59 kDa, Protein from Saccharomyces cerevisiae)
- Vacuolar import and degradation protein 24 (41 kDa, Protein from Saccharomyces cerevisiae)
- Fructose-bisphosphatase (38 kDa, Protein from Saccharomyces cerevisiae)
- Bj4_g0018240.mrna.1.cds.1 (108 kDa, Protein from Saccharomyces cerevisiae)
- Substrate receptor scaffolding module of yeast chelator-gid sr4 comprising gid1, gid4, gid5, gid8 and gid9 bound to fbp1 (310 kDa, Complex from Saccharomyces cerevisiae)
- Vacuolar import and degradation protein 28 (105 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Catalytic module of yeast Chelator-GID SR4 E3 ubiquitin ligase
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7ns4
Q-score: 0.498
Sherpa D, Chrustowicz J, Qiao S, Langlois CR, Hehl LA, Gottemukkala KV, Hansen FM, Karayel O, von Gronau S, Prabu JR, Mann M, Alpi AF, Schulman BA
Mol Cell (2021) 81 pp. 2445- [ Pubmed: 33905682 DOI: doi:10.1016/j.molcel.2021.03.025 ]
- Catalytic module of yeast chelator-gid sr4 comprising gid2 and gid9 (110 kDa, Complex from Saccharomyces cerevisiae)
- Protein fyv10 (59 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- E3 ubiquitin-protein ligase rmd5 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
Supramolecular assembly module of yeast Chelator-GID SR4 E3 ubiquitin ligase
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7nsb
Q-score: 0.5
Sherpa D, Chrustowicz J, Qiao S, Langlois CR, Hehl LA, Gottemukkala KV, Hansen FM, Karayel O, von Gronau S, Prabu JR, Mann M, Alpi AF, Schulman BA
Mol Cell (2021) 81 pp. 2445- [ Pubmed: 33905682 DOI: doi:10.1016/j.molcel.2021.03.025 ]
- Glucose-induced degradation protein 8 (55 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Glucose-induced degradation protein 7 (84 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Vacuolar import and degradation protein 30 (108 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Supramolecular assembly module of yeast chelator-gid sr4 comprising gid1, gid8 and a homodimer of gid7 (330 kDa, Complex from Saccharomyces cerevisiae)
Substrate receptor scaffolding module of human CTLH E3 ubiquitin ligase
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7nsc
Q-score: 0.515
Sherpa D, Chrustowicz J, Qiao S, Langlois CR, Hehl LA, Gottemukkala KV, Hansen FM, Karayel O, von Gronau S, Prabu JR, Mann M, Alpi AF, Schulman BA
Mol Cell (2021) 81 pp. 2445- [ Pubmed: 33905682 DOI: doi:10.1016/j.molcel.2021.03.025 ]
- Ran-binding protein 9 (77 kDa, Protein from Homo sapiens)
- Glucose-induced degradation protein 8 homolog (30 kDa, Protein from Homo sapiens)
- Isoform 2 of armadillo repeat-containing protein 8 (74 kDa, Protein from Homo sapiens)
- Glucose-induced degradation protein 4 homolog (33 kDa, Protein from Homo sapiens)
- Srs module of human ctlh complex comprising ranbp9, twa1, armc8 and hgid4 (210 kDa, Complex from Homo sapiens)
Structure of active GID E3 ubiquitin ligase complex minus Gid2 and delta Gid9 RING domain
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6swy
Q-score: 0.554
Qiao S, Langlois CR, Chrustowicz J, Sherpa D, Karayel O, Hansen FM, Beier V, von Gronau S, Bollschweiler D, Schafer T, Alpi AF, Mann M, Rajan Prabu J, Schulman BA
Mol Cell (2020) 77 pp. 1-14 [ Pubmed: 31708416 DOI: doi:10.1016/j.molcel.2019.10.009 ]
- Vacuolar import and degradation protein 30,vacuolar import and degradation protein 30,vacuolar import and degradation protein 30,vacuolar import and degradation protein 30,vacuolar import and degradation protein 30,vacuolar import and degradation protein 30,vacuolar import and degradation protein 30,vacuolar import and degradation protein 30,vacuolar import and degradation protein 30,vacuolar import and degradation protein 30,vacuolar import and degradation protein 30,vacuolar import and degradation protein 30,vacuolar import and degradation protein 30,vacuolar import and degradation protein 30,vacuolar import and degradation protein 30,vacuolar import and degradation protein 30 (117 kDa, Protein from Saccharomyces cerevisiae S288c)
- Protein fyv10,protein fyv10,protein fyv10,protein fyv10,protein fyv10,protein fyv10,protein fyv10 (54 kDa, Protein from Saccharomyces cerevisiae S288c)
- Gidsr4 minus gid2/delta gid9ring (Complex from Saccharomyces cerevisiae S288c)
- Glucose-induced degradation protein 8 (51 kDa, Protein from Saccharomyces cerevisiae S288c)
- Vacuolar import and degradation protein 28 (105 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Vacuolar import and degradation protein 24 (41 kDa, Protein from Saccharomyces cerevisiae S288c)