SARS-CoV-2 Spike bound to Nb6 in closed conformation
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 7kkk
Q-score: 0.463
Schoof M, Faust B, Saunders RA, Sangwan S, Rezelj V, Hoppe N, Boone M, Billesbolle CB, Puchades C, Azumaya CM, Kratochvil HT, Zimanyi M, Deshpande I, Liang J, Dickinson S, Nguyen HC, Chio CM, Merz GE, Thompson MC, Diwanji D, Schaefer K, Anand AA, Dobzinski N, Zha BS, Simoneau CR, Leon K, White KM, Chio US, Gupta M, Jin M, Li F, Liu Y, Zhang K, Bulkley D, Sun M, Smith AM, Rizo AN, Moss F, Brilot AF, Pourmal S, Trenker R, Pospiech T, Gupta S, Barsi-Rhyne B, Belyy V, Barile-Hill AW, Nock S, Liu Y, Krogan NJ, Ralston CY, Swaney DL, Garcia-Sastre A, Ott M, Vignuzzi M, QCRG Structural Biology Consortium, Walter P, Manglik A
Science (2020) 370 pp. 1473-1479 [ Pubmed: 33154106 DOI: doi:10.1126/science.abe3255 ]
- Synthetic nanobody nb6 (12 kDa, Protein from synthetic construct)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Spike s2p glycoprotein (Complex from Severe acute respiratory syndrome coronavirus 2)
- Complex of spike s2p glycoprotein with synthetic nanobody nb6 (Complex)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Synthetic nanobody nb6 (Complex from synthetic construct)
SARS-CoV-2 Spike bound to mNb6 in closed conformation
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 7kkl
Q-score: 0.514
Schoof M, Faust B, Saunders RA, Sangwan S, Rezelj V, Hoppe N, Boone M, Billesbolle CB, Puchades C, Azumaya CM, Kratochvil HT, Zimanyi M, Deshpande I, Liang J, Dickinson S, Nguyen HC, Chio CM, Merz GE, Thompson MC, Diwanji D, Schaefer K, Anand AA, Dobzinski N, Zha BS, Simoneau CR, Leon K, White KM, Chio US, Gupta M, Jin M, Li F, Liu Y, Zhang K, Bulkley D, Sun M, Smith AM, Rizo AN, Moss F, Brilot AF, Pourmal S, Trenker R, Pospiech T, Gupta S, Barsi-Rhyne B, Belyy V, Barile-Hill AW, Nock S, Liu Y, Krogan NJ, Ralston CY, Swaney DL, Garcia-Sastre A, Ott M, Vignuzzi M, QCRG Structural Biology Consortium, Walter P, Manglik A
Science (2020) 370 pp. 1473-1479 [ Pubmed: 33154106 DOI: doi:10.1126/science.abe3255 ]
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Synthetic nanobody mnb6 (13 kDa, Protein from synthetic construct)
- Spike s2p glycoprotein (Complex from Severe acute respiratory syndrome coronavirus 2)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Synthetic nanobody mnb6 (Complex from synthetic construct)
- Complex of spike s2p glycoprotein with synthetic nanobody mnb6 (Complex)
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 6o81
Q-score: 0.324
Kenner LR, Anand AA, Nguyen HC, Myasnikov AG, Klose CJ, McGeever LA, Tsai JC, Miller-Vedam LE, Walter P, Frost A
Science (2019) 364 pp. 491-495 [ DOI: doi:10.1126/science.aaw2922 Pubmed: 31048491 ]
- Translation initiation factor eif-2b subunit gamma (50 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 2 subunit 1 (36 kDa, Protein from Homo sapiens)
- Translation initiation factor eif-2b subunit alpha (33 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 2 subunit 3 (51 kDa, Protein from Homo sapiens)
- Translation initiation factor eif2 beta-subunit (1 kDa, Protein from Homo sapiens)
- Translation initiation factor eif-2b subunit delta (57 kDa, Protein from Homo sapiens)
- Eif2b bound to eif2 (Complex from Homo sapiens)
- 2-(4-chloranylphenoxy)-~{n}-[4-[2-(4-chloranylphenoxy)ethanoylamino]cyclohexyl]ethanamide (451 Da, Ligand)
- Translation initiation factor eif-2b subunit beta (41 kDa, Protein from Homo sapiens)
- Translation initiation factor eif-2b subunit epsilon (80 kDa, Protein from Homo sapiens)
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 6o85
Q-score: 0.359
Kenner LR, Anand AA, Nguyen HC, Myasnikov AG, Klose CJ, McGeever LA, Tsai JC, Miller-Vedam LE, Walter P, Frost A
Science (2019) 364 pp. 491-495 [ DOI: doi:10.1126/science.aaw2922 Pubmed: 31048491 ]
- Translation initiation factor eif-2b subunit gamma (50 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 2 subunit 1 (36 kDa, Protein from Homo sapiens)
- Translation initiation factor eif-2b subunit alpha (33 kDa, Protein from Homo sapiens)
- Translation initiation factor eif-2b subunit epsilon (80 kDa, Protein from Homo sapiens)
- Translation initiation factor eif-2b subunit delta (57 kDa, Protein from Homo sapiens)
- Eif2 bound to eif2b (Cellular component from Homo sapiens)
- Eukaryotic translation initiation factor 2 subunit 3 (51 kDa, Protein from Homo sapiens)
- 2-(4-chloranylphenoxy)-~{n}-[4-[2-(4-chloranylphenoxy)ethanoylamino]cyclohexyl]ethanamide (451 Da, Ligand)
- Translation initiation factor eif-2b subunit beta (41 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 2 subunit beta (1 kDa, Protein from Homo sapiens)
Electron cryo-microscopy of the eukaryotic translation initiation factor 2B from Homo sapiens
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6caj
Q-score: 0.453
Tsai JC, Miller-Vedam LE, Anand AA, Jaishankar P, Nguyen HC, Renslo AR, Frost A, Walter P
Science (2018) 359 [ DOI: doi:10.1126/science.aaq0939 Pubmed: 29599213 ]
- Translation initiation factor eif-2b subunit gamma (50 kDa, Protein from Homo sapiens)
- Translation initiation factor eif-2b subunit alpha (33 kDa, Protein from Homo sapiens)
- Translation initiation factor eif-2b subunit epsilon (80 kDa, Protein from Homo sapiens)
- Translation initiation factor eif-2b subunit delta (57 kDa, Protein from Homo sapiens)
- 2-(4-chloranylphenoxy)-~{n}-[4-[2-(4-chloranylphenoxy)ethanoylamino]cyclohexyl]ethanamide (451 Da, Ligand)
- Translation initiation factor eif-2b subunit beta (41 kDa, Protein from Homo sapiens)
- Translation initiation factor eif-2b decamer (Complex from Homo sapiens)