RAD51 nucleoprotein filament on abasic single-stranded DNA
Hanthi YW, Ramirez-Otero MA, Appleby R, De Antoni A, Joudeh L, Sannino V, Waked S, Ardizzoia A, Barra V, Fachinetti D, Pellegrini L, Costanzo V
Mol Cell (2024) 84 pp. 3026-3043.e11 [ Pubmed: 39178838 DOI: doi:10.1016/j.molcel.2024.07.004 ]
- Calcium ion (40 Da, Ligand)
- Dna (5'-d(p*gp*gp*(3dr)p*ap*tp*(3dr)p*cp*ap*(3dr)p*tp*gp*(3dr)p*tp*ap*(3dr)p*ap*cp*(3dr)p*tp*gp*(3dr)p*gp*c)-3') (6 kDa, DNA from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna repair protein rad51 homolog 1 (37 kDa, Protein from Homo sapiens)
- Rad51 nucleoprotein filament (Complex from Homo sapiens)
RAD51 nucleoprotein filament on double-stranded abasic DNA
Hanthi YW, Ramirez-Otero MA, Appleby R, De Antoni A, Joudeh L, Sannino V, Waked S, Ardizzoia A, Barra V, Fachinetti D, Pellegrini L, Costanzo V
Mol Cell (2024) 84 pp. 3026-3043.e11 [ Pubmed: 39178838 DOI: doi:10.1016/j.molcel.2024.07.004 ]
- Dna (5'-d(p*tp*gp*(3dr)p*tp*gp*(3dr)p*tp*gp*(3dr)p*tp*gp*(3dr)p*tp*gp*(3dr)p*tp*gp*(3dr)p*tp*gp*(3dr)p*tp*g)-3') (6 kDa, DNA from Homo sapiens)
- Dna (Complex from Homo sapiens)
- Dna repair protein rad51 homolog 1 (37 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Dna (5'-d(p*cp*ap*cp*cp*ap*cp*cp*ap*cp*cp*ap*cp*cp*ap*cp*cp*ap*cp*cp*ap*cp*cp*a)-3') (6 kDa, DNA from Homo sapiens)
- Rad51 nucleoprotein filament on double-stranded abasic dna (Complex)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna repair protein (Complex from Homo sapiens)
CryoEM micrographs of RAD51 filaments on ssDNA bound by the BRCA2 c-terminus
Appleby R, Joudeh L, Cobbett K, Pellegrini L
Nat Commun (2023) 14 [ DOI: 10.1038/s41467-023-42830-1 Pubmed: 37919288 ]
Image sets:
CryoEM micrographs of RAD51 filaments on dsDNA bound by the BRCA2 c-terminus
Appleby R, Joudeh L, Cobbett K, Pellegrini L
Nat Commun (2023) 14 [ DOI: 10.1038/s41467-023-42830-1 Pubmed: 37919288 ]
Image sets:
RAD51 filament on ssDNA bound by the BRCA2 c-terminus
Resolution: 2.61 Å
EM Method: Helical reconstruction
Fitted PDBs: 8pbc
Q-score: 0.551
Appleby R, Joudeh L, Cobbett K, Pellegrini L
Nat Commun (2023) 14 pp. 7003-7003 [ DOI: doi:10.1038/s41467-023-42830-1 Pubmed: 37919288 ]
- Dna (30-mer) (Complex from synthetic construct)
- Rad51 filament formed on ssdna and bound with a peptide corresponding to the brca2 c-terminus (Complex)
- Breast cancer type 2 susceptibility protein (5 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Dna repair protein rad51 homolog 1 (37 kDa, Protein from Homo sapiens)
- Dna repair protein rad51 homolog 1 (Complex from Homo sapiens)
- Dna (30-mer) (9 kDa, DNA from synthetic construct)
- Breast cancer type 2 susceptibility protein (Complex from Homo sapiens)
RAD51 filament on dsDNA bound by the BRCA2 c-terminus
Resolution: 2.83 Å
EM Method: Helical reconstruction
Fitted PDBs: 8pbd
Q-score: 0.535
Appleby R, Joudeh L, Cobbett K, Pellegrini L
Nat Commun (2023) 14 pp. 7003-7003 [ DOI: doi:10.1038/s41467-023-42830-1 Pubmed: 37919288 ]
- Rad51 filament formed on dsdna and bound with a peptide corresponding to the brca2 c-terminus (Complex)
- Breast cancer type 2 susceptibility protein (5 kDa, Protein from Homo sapiens)
- Dna strand 2 (7 kDa, DNA from synthetic construct)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna repair protein rad51 homolog 1 (37 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Dna strand 1 (8 kDa, DNA from synthetic construct)
- Dna repair protein rad51 homolog 1 (Complex from Homo sapiens)
- Breast cancer type 2 susceptibility protein (Complex from Homo sapiens)
- Dna strand 1 and 2 (Complex from synthetic construct)
CryoEM micrographs collected on a RAD51-ATP-dsDNA filament sample
Appleby R, Bollschweiler D, Chirgadze DY, Joudeh L, Pellegrini L
iScience (2023) 26 [ DOI: 10.1016/j.isci.2023.106689 Pubmed: 37216117 ]
Image sets:
CryoEM micrographs collected on a RAD51-ADP filament sample
Appleby R, Bollschweiler D, Chirgadze DY, Joudeh L, Pellegrini L
iScience (2023) 26 [ DOI: 10.1016/j.isci.2023.106689 Pubmed: 37216117 ]
Image sets:
CryoEM structure of the presynaptic RAD51 nucleoprotein filament in the presence of ATP and Ca2+
Resolution: 3.8 Å
EM Method: Helical reconstruction
Fitted PDBs: 8bq2
Q-score: 0.457
Appleby R, Bollschweiler D, Chirgadze DY, Joudeh L, Pellegrini L
iScience (2023) 26 pp. 106689-106689 [ DOI: doi:10.1016/j.isci.2023.106689 Pubmed: 37216117 ]
- Dna (30-mer) (9 kDa, DNA from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Nucleoprotein filament of human rad51 bound to single-stranded dna 60mer in the presence of atp and ca2+ (Complex from Homo sapiens)
- Dna repair protein rad51 homolog 1 (37 kDa, Protein from Homo sapiens)
CryoEM structure of the post-synaptic RAD51 nucleoprotein filament in the presence of ATP and Ca2+
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8br2
Q-score: 0.573
Appleby R, Bollschweiler D, Chirgadze DY, Joudeh L, Pellegrini L
iScience (2023) 26 pp. 106689-106689 [ DOI: doi:10.1016/j.isci.2023.106689 Pubmed: 37216117 ]
- Dna (5'-d(p*gp*cp*gp*ap*gp*cp*tp*cp*gp*ap*tp*gp*cp*ap*cp*cp*tp*cp*cp*a)-3') (6 kDa, DNA from Homo sapiens)
- Water (18 Da, Ligand)
- Dna repair protein rad51 homolog 1 (37 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Dna (5'-d(p*tp*gp*gp*ap*gp*gp*tp*gp*cp*ap*tp*cp*gp*ap*gp*cp*tp*cp*gp*c)-3') (6 kDa, DNA from Homo sapiens)
- Post-synaptic rad51 nucleoprotein filament in the presence of atp and ca2+ (Complex from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)