Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8pm0
Q-score: 0.546
Krischuns T, Arragain B, Isel C, Paisant S, Budt M, Wolff T, Cusack S, Naffakh N
Nat Commun (2024) 15 pp. 1064-1064 [ Pubmed: 38316757 DOI: doi:10.1038/s41467-024-45205-2 ]
- Rna pol ii ctd 6 repeats (site 1a/2a) (4 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Influenza a/h7n9 polymerase in replicase-like conformation in pre-initiation state with pol ii ps5 ctd peptide mimic bound in site 1a/2a (Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (89 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 51-mer vrna loop (v51_mut_s) (16 kDa, RNA from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase acidic protein (84 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8pnp
Q-score: 0.611
Krischuns T, Arragain B, Isel C, Paisant S, Budt M, Wolff T, Cusack S, Naffakh N
Nat Commun (2024) 15 pp. 1064-1064 [ Pubmed: 38316757 DOI: doi:10.1038/s41467-024-45205-2 ]
- Rna pol ii ctd 6 repeats (site 1a/2a) (4 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Influenza a/h7n9 polymerase in replicase-like conformation in pre-initiation state with pol ii ps5 ctd peptide mimic bound in site 1a/2a (Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (89 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 51-mer vrna loop (v51_mut_s) (16 kDa, RNA from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase acidic protein (84 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 8pnq
Q-score: 0.586
Krischuns T, Arragain B, Isel C, Paisant S, Budt M, Wolff T, Cusack S, Naffakh N
Nat Commun (2024) 15 pp. 1064-1064 [ Pubmed: 38316757 DOI: doi:10.1038/s41467-024-45205-2 ]
- Rna pol ii ctd 6 repeats (site 1a/2a) (4 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine (483 Da, Ligand)
- Replication product (10 kDa, RNA from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Influenza a/h7n9 polymerase in replicase-like conformation in pre-initiation state with pol ii ps5 ctd peptide mimic bound in site 1a/2a (Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (89 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 51-mer vrna loop (v51_mut_s) (16 kDa, RNA from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase acidic protein (84 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
Influenza A/H7N9 polymerase symmetric dimer bound to the promoter (PA K289A/C489R)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8poh
Q-score: 0.497
Krischuns T, Arragain B, Isel C, Paisant S, Budt M, Wolff T, Cusack S, Naffakh N
Nat Commun (2024) 15 pp. 1064-1064 [ Pubmed: 38316757 DOI: doi:10.1038/s41467-024-45205-2 ]
- Magnesium ion (24 Da, Ligand)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase acidic protein (84 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 51-mer vrna loop (v51_mut_s) (16 kDa, RNA from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase acidic protein, rna-directed rna polymerase catalytic subunit and polymerase basic protein 2 (Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 51-mer vrna loop (v51_mut_s) (Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (88 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Influenza a/h7n9 polymerase in replicase-like conformation in pre-initiation state with pol ii ps5 ctd peptide mimic bound in site 1a/2a (Complex)
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 8r3k
Q-score: 0.512
Krischuns T, Arragain B, Isel C, Paisant S, Budt M, Wolff T, Cusack S, Naffakh N
Nat Commun (2024) 15 pp. 1064-1064 [ Pubmed: 38316757 DOI: doi:10.1038/s41467-024-45205-2 ]
- Rna pol ii ctd 6 repeats (site 1a/2a) (4 kDa, Protein from Homo sapiens)
- Rna product (11 kDa, RNA from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Magnesium ion (24 Da, Ligand)
- Heterotrimeric influenza polymerase (Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Pyrophosphate 2- (175 Da, Ligand)
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 51-mer vrna loop (v51_mut_s) (16 kDa, RNA from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (89 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 8r3l
Q-score: 0.464
Krischuns T, Arragain B, Isel C, Paisant S, Budt M, Wolff T, Cusack S, Naffakh N
Nat Commun (2024) 15 pp. 1064-1064 [ Pubmed: 38316757 DOI: doi:10.1038/s41467-024-45205-2 ]
- Rna pol ii ctd 6 repeats (site 1a/2a) (4 kDa, Protein from Homo sapiens)
- Heterotrimeric influenza polymerase (Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (89 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 3' vrna end (51-mer vrna loop) (16 kDa, RNA from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase acidic protein (84 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))