La Crosse virus polymerase at transcription capped RNA cleavage stage
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7orj
Q-score: 0.427
Arragain B, Durieux Trouilleton Q, Baudin F, Provaznik J, Azevedo N, Cusack S, Schoehn G, Malet H
Nat Commun (2022) 13 pp. 902-902 [ Pubmed: 35173159 DOI: doi:10.1038/s41467-022-28428-z ]
- Zinc ion (65 Da, Ligand)
- Rna (5'-r(p*cp*up*up*gp*gp*up*ap*gp*up*ap*cp*ap*cp*up*ap*cp*u)-3') (7 kDa, RNA from La Crosse orthobunyavirus)
- La crosse virus polymerase at transcription capped rna cleavage stage (284 kDa, Complex)
- 5' capped rna (4 kDa, RNA from La Crosse orthobunyavirus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Rna (5'-r(p*ap*cp*gp*ap*gp*up*gp*up*cp*gp*up*ap*cp*cp*ap*ap*g)-3') (5 kDa, RNA from La Crosse orthobunyavirus)
- Rna-directed rna polymerase l (264 kDa, Protein from Bunyavirus La Crosse)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7ork
Q-score: 0.568
Arragain B, Durieux Trouilleton Q, Baudin F, Provaznik J, Azevedo N, Cusack S, Schoehn G, Malet H
Nat Commun (2022) 13 pp. 902-902 [ Pubmed: 35173159 DOI: doi:10.1038/s41467-022-28428-z ]
- Rna (5'-d(*(gtg))-r(p*a)-3') (4 kDa, RNA from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- La crosse virus polymerase in transcription mode with cleaved capped rna entering the polymerase active site (284 kDa, Complex)
- Rna (5'-r(p*ap*cp*gp*ap*gp*up*gp*up*cp*gp*up*ap*cp*cp*ap*ap*g)-3') (5 kDa, RNA from La Crosse virus)
- Rna (5'-r(p*ap*cp*up*up*gp*gp*up*ap*gp*up*ap*cp*ap*cp*up*ap*cp*u)-3') (7 kDa, RNA from La Crosse virus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Rna-directed rna polymerase l (264 kDa, Protein from Bunyavirus La Crosse)
- Water (18 Da, Ligand)
La Crosse virus polymerase at transcription initiation stage
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7orl
Q-score: 0.474
Arragain B, Durieux Trouilleton Q, Baudin F, Provaznik J, Azevedo N, Cusack S, Schoehn G, Malet H
Nat Commun (2022) 13 pp. 902-902 [ Pubmed: 35173159 DOI: doi:10.1038/s41467-022-28428-z ]
- Zinc ion (65 Da, Ligand)
- La crosse virus polymerase at transcription initiation (284 kDa, Complex from La Crosse virus)
- Pyrophosphate 2- (175 Da, Ligand)
- Rna (5'-r(p*cp*up*up*gp*gp*up*ap*gp*up*ap*cp*ap*cp*up*ap*cp*u)-3') (7 kDa, RNA from La Crosse virus)
- 7-methyl-guanosine-5'-triphosphate-5'-guanosine (803 Da, Ligand)
- Rna (5'-r(p*ap*cp*gp*ap*gp*up*gp*up*cp*gp*up*ap*cp*cp*ap*ap*g)-3') (5 kDa, RNA from La Crosse virus)
- Magnesium ion (24 Da, Ligand)
- Rna-directed rna polymerase l (264 kDa, Protein from La Crosse virus)
- Rna (5'-d(*(gtg))-r(p*ap*ap*up*ap*cp*up*ap*up*ap*gp*up*a)-3') (4 kDa, RNA from La Crosse virus)
La Crosse virus polymerase at transcription early-elongation stage
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7orm
Q-score: 0.495
Arragain B, Durieux Trouilleton Q, Baudin F, Provaznik J, Azevedo N, Cusack S, Schoehn G, Malet H
Nat Commun (2022) 13 pp. 902-902 [ Pubmed: 35173159 DOI: doi:10.1038/s41467-022-28428-z ]
- La crosse virus polymerase at transcription early elongation (287 kDa, Complex from La Crosse virus)
- Zinc ion (65 Da, Ligand)
- Rna (5'-r(p*ap*cp*gp*ap*gp*up*gp*up*cp*gp*up*ap*cp*c)-3') (5 kDa, RNA from La Crosse virus)
- Rna (5'-r(p*up*ap*up*ap*ap*up*ap*gp*up*ap*gp*up*gp*up*a)-3') (6 kDa, RNA from La Crosse virus)
- Rna (5'-r(p*ap*ap*ap*gp*up*ap*cp*ap*cp*up*ap*cp*u)-3') (7 kDa, RNA from La Crosse virus)
- Magnesium ion (24 Da, Ligand)
- Rna-directed rna polymerase l (264 kDa, Protein from Bunyavirus La Crosse)
Cryo-EM map of La Crosse virus polymerase at pre-initiation stage
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 6z6g
Q-score: 0.499
Arragain B, Effantin G, Gerlach P, Reguera J, Schoehn G, Cusack S, Malet H
Nat Commun (2020) 11 pp. 3590-3590 [ DOI: doi:10.1038/s41467-020-17349-4 Pubmed: 32681014 ]
- 5'vrna 9-16 (2 kDa, RNA from La Crosse orthobunyavirus)
- La crosse virus polymerase at pre-initiation stage (265 kDa, Complex)
- La crosse virus polymerase at pre-initiation stage (Complex from Bunyavirus La Crosse)
- Zinc ion (65 Da, Ligand)
- 3'vrna 1-16 (5 kDa, RNA from La Crosse orthobunyavirus)
- La crosse virus polymerase at pre-initiation stage (Complex from La Crosse orthobunyavirus)
- Magnesium ion (24 Da, Ligand)
- 5'vrna 1-10 (3 kDa, RNA from La Crosse orthobunyavirus)
- Rna-directed rna polymerase l (266 kDa, Protein from Bunyavirus La Crosse)