Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 9gcs
Q-score: 0.276
Gjorgjevikj D, Kumar N, Wang B, Hilal T, Said N, Loll B, Artsimovitch I, Sen R, Wahl MC
Nat Commun (2025) 16 pp. 550-550 [ Pubmed: 39788982 DOI: doi:10.1038/s41467-025-55897-9 ]
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Polarity suppression protein (21 kDa, Protein from Enterobacteria phage P4)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transcription termination factor rho (Complex from Escherichia coli)
- Polarity suppression protein (Complex from Enterobacteria phage P4)
- Complex of e. coli transcription termination factor rho with atp and p4 polarity suppression protein psu (Complex)
Rho-ATP-Psu complex II expanded
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 9gct
Q-score: 0.274
Gjorgjevikj D, Kumar N, Wang B, Hilal T, Said N, Loll B, Artsimovitch I, Sen R, Wahl MC
Nat Commun (2025) 16 pp. 550-550 [ Pubmed: 39788982 DOI: doi:10.1038/s41467-025-55897-9 ]
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Polarity suppression protein (21 kDa, Protein from Enterobacteria phage P4)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transcription termination factor rho (Complex from Escherichia coli)
- Polarity suppression protein (Complex from Enterobacteria phage P4)
- Complex of e. coli transcription termination factor rho with atp and p4 polarity suppression protein psu (Complex)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 9gcu
Q-score: 0.475
Gjorgjevikj D, Kumar N, Wang B, Hilal T, Said N, Loll B, Artsimovitch I, Sen R, Wahl MC
Nat Commun (2025) 16 pp. 550-550 [ Pubmed: 39788982 DOI: doi:10.1038/s41467-025-55897-9 ]
- Magnesium ion (24 Da, Ligand)
- Complex of e. coli transcription termination factor rho p167l variant with atp gamma s (Complex)
- Water (18 Da, Ligand)
- Transcription termination factor rho p167l variant (Complex from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8peu
Q-score: 0.294
Gjorgjevikj D, Kumar N, Wang B, Hilal T, Said N, Loll B, Artsimovitch I, Sen R, Wahl MC
Nat Commun (2025) 16 pp. 550-550 [ Pubmed: 39788982 DOI: doi:10.1038/s41467-025-55897-9 ]
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Polarity suppression protein (21 kDa, Protein from Enterobacteria phage P4)
- Complex of e. coli transcription termination factor rho with the atp analog, atpgammas and p4 polarity suppression protein psu (Complex)
- Magnesium ion (24 Da, Ligand)
- Transcription termination factor rho (Complex from Escherichia coli)
- Polarity suppression protein (Complex from Enterobacteria phage P4)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 8pew
Q-score: 0.216
Gjorgjevikj D, Kumar N, Wang B, Hilal T, Said N, Loll B, Artsimovitch I, Sen R, Wahl MC
Nat Commun (2025) 16 pp. 550-550 [ Pubmed: 39788982 DOI: doi:10.1038/s41467-025-55897-9 ]
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Polarity suppression protein (21 kDa, Protein from Enterobacteria phage P4)
- Complex of e. coli transcription termination factor rho with the atp analog, atpgammas and p4 polarity suppression protein psu (Complex)
- Magnesium ion (24 Da, Ligand)
- Transcription termination factor rho (Complex from Escherichia coli)
- Polarity suppression protein (Complex from Enterobacteria phage P4)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8pex
Q-score: 0.373
Gjorgjevikj D, Kumar N, Wang B, Hilal T, Said N, Loll B, Artsimovitch I, Sen R, Wahl MC
Nat Commun (2025) 16 pp. 550-550 [ Pubmed: 39788982 DOI: doi:10.1038/s41467-025-55897-9 ]
- Polarity suppression protein (21 kDa, Protein from Enterobacteria phage P4)
- Magnesium ion (24 Da, Ligand)
- Transcription termination factor rho (Complex from Escherichia coli)
- Polarity suppression protein (Complex from Enterobacteria phage P4)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Complex of e. coli transcription termination factor rho p167l variant with the atp analog, atpgammas and p4 polarity suppression protein psu (Complex)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
Rho P167L-ATPgS-Psu complex II
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8pey
Q-score: 0.407
Gjorgjevikj D, Kumar N, Wang B, Hilal T, Said N, Loll B, Artsimovitch I, Sen R, Wahl MC
Nat Commun (2025) 16 pp. 550-550 [ Pubmed: 39788982 DOI: doi:10.1038/s41467-025-55897-9 ]
- Polarity suppression protein (21 kDa, Protein from Enterobacteria phage P4)
- Magnesium ion (24 Da, Ligand)
- Transcription termination factor rho (Complex from Escherichia coli)
- Polarity suppression protein (Complex from Enterobacteria phage P4)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Complex of e. coli transcription termination factor rho p167l variant with the atp analog, atpgammas and p4 polarity suppression protein psu (Complex)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
E. coli RNA polymerase paused at ops site
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8pdy
Q-score: 0.409
Zuber PK, Said N, Hilal T, Wang B, Loll B, Gonzalez-Higueras J, Ramirez-Sarmiento CA, Belogurov GA, Artsimovitch I, Wahl MC, Knauer SH
Nat Commun (2024) 15 pp. 3040-3040 [ DOI: doi:10.1038/s41467-024-47368-4 Pubmed: 38589445 ]
- Transcription elongation complex paused at ops site, assembled on fully complementary nucleic acid scaffold (399 kDa, Complex)
- Dna and rna (Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Rna (5'-r(p*up*gp*gp*cp*gp*gp*up*ap*gp*cp*gp*u)-3') (5 kDa, RNA from Escherichia coli)
- Non-template dna (35-mer) (12 kDa, DNA from Escherichia coli)
- Template dna (35-mer) (12 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Transcription complex (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8pen
Q-score: 0.451
Zuber PK, Said N, Hilal T, Wang B, Loll B, Gonzalez-Higueras J, Ramirez-Sarmiento CA, Belogurov GA, Artsimovitch I, Wahl MC, Knauer SH
Nat Commun (2024) 15 pp. 3040-3040 [ DOI: doi:10.1038/s41467-024-47368-4 Pubmed: 38589445 ]
- Rna (5 kDa, RNA from Escherichia coli)
- Dna and rna (Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Transcription complex paused at ops site with fully recruited rfah (417 kDa, Complex)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Non-template dna (12 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Template dna (12 kDa, DNA from Escherichia coli)
- Transcription complex (Complex from Escherichia coli)
- Transcription antitermination protein rfah (18 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8pfg
Q-score: 0.471
Zuber PK, Said N, Hilal T, Wang B, Loll B, Gonzalez-Higueras J, Ramirez-Sarmiento CA, Belogurov GA, Artsimovitch I, Wahl MC, Knauer SH
Nat Commun (2024) 15 pp. 3040-3040 [ DOI: doi:10.1038/s41467-024-47368-4 Pubmed: 38589445 ]
- Rna (5 kDa, RNA from Escherichia coli)
- Dna and rna (Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Template dna (12 kDa, DNA from Escherichia coli)
- Transcription antitermination protein rfah (18 kDa, Protein from Escherichia coli)
- Non-template dna (12 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Transcription complex (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Transcription complex paused at ops site, assembled on not fully complementary nucleic acid scaffold, bound to disulfide-bridged (autoinhibited) rfah (417 kDa, Complex)