Cryo-EM structure of CasLambda (Cas12l) bound to crRNA and DNA
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 8dc2
Q-score: 0.491
Al-Shayeb B, Skopintsev P, Soczek KM, Stahl EC, Li Z, Groover E, Smock D, Eggers AR, Pausch P, Cress BF, Huang CJ, Staskawicz B, Savage DF, Jacobsen SE, Banfield JF, Doudna JA
Cell (2022) 185 pp. 4574-4586.e16 [ DOI: doi:10.1016/j.cell.2022.10.020 Pubmed: 36423580 ]
- Dna nts (14 kDa, DNA from uncultured virus)
- Caslambda (87 kDa, Protein from uncultured virus)
- Caslambda-crrna-dsdna ternary structure (Complex from uncultured virus)
- Rna (51-mer) (16 kDa, RNA from uncultured virus)
- Dna ts (14 kDa, DNA from uncultured virus)
Cryo-EM structure of CasPhi-2 (Cas12j) bound to crRNA and DNA
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 7lys
Q-score: 0.517
Pausch P, Soczek KM, Herbst DA, Tsuchida CA, Al-Shayeb B, Banfield JF, Nogales E, Doudna JA
Nat Struct Mol Biol (2021) 28 pp. 652-661 [ DOI: doi:10.1038/s41594-021-00632-3 Pubmed: 34381246 ]
- Crrna (14 kDa, RNA from Phage #D)
- Cryo-em map of casphi bound to crrna and dna (124 kDa, Complex from Biggievirus Mos11)
- Ts-dna (12 kDa, DNA from Phage #D)
- Casphi-2 (86 kDa, Protein from Biggievirus Mos11)
- Nts-dna (11 kDa, DNA from Phage #D)
Cryo-EM structure of CasPhi-2 (Cas12j) bound to crRNA and Phosphorothioate-DNA
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7lyt
Q-score: 0.524
Pausch P, Soczek KM, Herbst DA, Tsuchida CA, Al-Shayeb B, Banfield JF, Nogales E, Doudna JA
Nat Struct Mol Biol (2021) 28 pp. 652-661 [ DOI: doi:10.1038/s41594-021-00632-3 Pubmed: 34381246 ]
- Ts-dna (13 kDa, DNA from Biggievirus Mos11)
- Nts-dna* (13 kDa, DNA from Biggievirus Mos11)
- Magnesium ion (24 Da, Ligand)
- Cryo-em map of casphi bound to crrna and phosphorothioate-dna in the presence of the nuclease magnesium cofactor (128 kDa, Complex from Biggievirus Mos11)
- Casphi (86 kDa, Protein from Biggievirus Mos11)
- Crrna (14 kDa, RNA from Biggievirus Mos11)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of CasPhi-2 (Cas12j) bound to crRNA
Resolution: 3.54 Å
EM Method: Single-particle
Fitted PDBs: 7m5o
Q-score: 0.389
Pausch P, Soczek KM, Herbst DA, Tsuchida CA, Al-Shayeb B, Banfield JF, Nogales E, Doudna JA
Nat Struct Mol Biol (2021) 28 pp. 652-661 [ DOI: doi:10.1038/s41594-021-00632-3 Pubmed: 34381246 ]
- Crrna (14 kDa, RNA from Phage #D)
- Cryo-em map of casphi bound to crrna (124 kDa, Complex from Biggievirus Mos11)
- Casphi (86 kDa, Protein from Biggievirus Mos11)
- Zinc ion (65 Da, Ligand)
Partial Cas6-RT-Cas1--Cas2 complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7kft
Q-score: 0.502
Wang JY, Hoel CM, Al-Shayeb B, Banfield JF, Brohawn SG, Doudna JA
Nat Commun (2021) 12 pp. 2571-2571 [ DOI: doi:10.1038/s41467-021-22900-y Pubmed: 33958590 ]
- Cas6-rt-cas1 (112 kDa, Protein from Thiomicrospira sp.)
- Cas6-rt-cas1--cas2 complex (470 kDa, Complex from Thiomicrospira sp.)
- Cas2 (11 kDa, Protein from Thiomicrospira sp.)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7kfu
Q-score: 0.314
Wang JY, Hoel CM, Al-Shayeb B, Banfield JF, Brohawn SG, Doudna JA
Nat Commun (2021) 12 pp. 2571-2571 [ DOI: doi:10.1038/s41467-021-22900-y Pubmed: 33958590 ]
- Cas6-rt-cas1 (112 kDa, Protein from Thiomicrospira sp.)
- Cas6-rt-cas1--cas2 complex (470 kDa, Complex from Thiomicrospira sp.)
- Cas2 (11 kDa, Protein from Thiomicrospira sp.)