Resolution: 3.7 Å
EM Method: Helical reconstruction
Fitted PDBs: 8s5h
Q-score: 0.408
McCorvie TJ, Adamoski D, Machado RAC, Tang J, Bailey HJ, Ferreira DSM, Strain-Damerell C, Basle A, Ambrosio ALB, Dias SMG, Yue WW
Nat Commun (2024) 15 pp. 2931-2931 [ DOI: doi:10.1038/s41467-024-46864-x Pubmed: 38575566 ]
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8s5i
Q-score: 0.396
McCorvie TJ, Adamoski D, Machado RAC, Tang J, Bailey HJ, Ferreira DSM, Strain-Damerell C, Basle A, Ambrosio ALB, Dias SMG, Yue WW
Nat Commun (2024) 15 pp. 2931-2931 [ DOI: doi:10.1038/s41467-024-46864-x Pubmed: 38575566 ]
Substrate-free levan utilisation machinery (utilisome)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8a9y
Q-score: 0.458
White JBR, Silale A, Feasey M, Heunis T, Zhu Y, Zheng H, Gajbhiye A, Firbank S, Basle A, Trost M, Bolam DN, van den Berg B, Ranson NA
Nature (2023) 618 pp. 583-589 [ DOI: doi:10.1038/s41586-023-06146-w Pubmed: 37286596 ]
- Susc homolog (115 kDa, Protein from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50))
- Magnesium ion (24 Da, Ligand)
- Substrate-free levan utilisation machinery (utilisome) (Complex from Bacteroides thetaiotaomicron)
- Glycoside hydrolase family 32 (59 kDa, Protein from Bacteroides thetaiotaomicron VPI-5482)
- Duf4960 domain-containing protein (50 kDa, Protein from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50))
Core SusCD transporter units from the levan utilisome with levan fructo-oligosaccharides DP 8-12
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8aa1
Q-score: 0.57
White JBR, Silale A, Feasey M, Heunis T, Zhu Y, Zheng H, Gajbhiye A, Firbank S, Basle A, Trost M, Bolam DN, van den Berg B, Ranson NA
Nature (2023) 618 pp. 583-589 [ DOI: doi:10.1038/s41586-023-06146-w Pubmed: 37286596 ]
- Magnesium ion (24 Da, Ligand)
- Levan utilisation machinery (utilisome) in the presence of levan fructo-oligosaccharides dp 8-12 (Complex from Bacteroides thetaiotaomicron VPI-5482)
- Susd homolog (66 kDa, Protein from Bacteroides thetaiotaomicron VPI-5482)
- Susc homolog (115 kDa, Protein from Bacteroides thetaiotaomicron VPI-5482)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8aa2
Q-score: 0.529
White JBR, Silale A, Feasey M, Heunis T, Zhu Y, Zheng H, Gajbhiye A, Firbank S, Basle A, Trost M, Bolam DN, van den Berg B, Ranson NA
Nature (2023) 618 pp. 583-589 [ DOI: doi:10.1038/s41586-023-06146-w Pubmed: 37286596 ]
- Glycoside hydrolase family 32 (59 kDa, Protein from Bacteroides thetaiotaomicron VPI-5482)
- Susd homolog (65 kDa, Protein from Bacteroides thetaiotaomicron VPI-5482)
- Magnesium ion (24 Da, Ligand)
- Inactive levan utilisation machinery (utilisome) with levan fructo-oligosaccharides dp 15-25 (Complex from Bacteroides thetaiotaomicron VPI-5482)
- Susc homolog (115 kDa, Protein from Bacteroides thetaiotaomicron VPI-5482)
- Duf4960 domain-containing protein (51 kDa, Protein from Bacteroides thetaiotaomicron VPI-5482)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8aa3
Q-score: 0.59
White JBR, Silale A, Feasey M, Heunis T, Zhu Y, Zheng H, Gajbhiye A, Firbank S, Basle A, Trost M, Bolam DN, van den Berg B, Ranson NA
Nature (2023) 618 pp. 583-589 [ DOI: doi:10.1038/s41586-023-06146-w Pubmed: 37286596 ]
- Inactive levan utilisation machinery (utilisome) in the presence of fructo-oligosaccharides dp 15-25 (Complex from Bacteroides thetaiotaomicron)
- Magnesium ion (24 Da, Ligand)
- Susd homolog (65 kDa, Protein from Bacteroides thetaiotaomicron VPI-5482)
- Susc homolog (115 kDa, Protein from Bacteroides thetaiotaomicron VPI-5482)
Ross J, McIver Z, Lambert T, Piergentili C, Bird JE, Gallagher KJ, Cruickshank FL, James P, Zarazua-Arvizu E, Horsfall LE, Waldron KJ, Wilson MD, Mackay CL, Basle A, Clarke DJ, Marles-Wright J
Sci Adv (2022) 8 pp. eabj4461-eabj4461 [ DOI: doi:10.1126/sciadv.abj4461 Pubmed: 35080974 ]
- Haliangium ochraceum encapsulated ferritin (Protein from Haliangium ochraceum)
- Encapsulin nano compartment (Complex from Haliangium ochraceum)
- Ternary complex of haliangium ochraceum encapsulin and encapsulated ferritin proteins (1 MDa, Complex from Haliangium ochraceum)
- Haliangium ochraceum encapsulin (Protein from Haliangium ochraceum)
Icosahedral cryo-EM reconstruction of Haliangium ochraceum encapsulin
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7odw
Q-score: 0.564
Ross J, McIver Z, Lambert T, Piergentili C, Bird JE, Gallagher KJ, Cruickshank FL, James P, Zarazua-Arvizu E, Horsfall LE, Waldron KJ, Wilson MD, Mackay CL, Basle A, Clarke DJ, Marles-Wright J
Sci Adv (2022) 8 pp. eabj4461-eabj4461 [ DOI: doi:10.1126/sciadv.abj4461 Pubmed: 35080974 ]
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7oe2
Q-score: 0.617
Ross J, McIver Z, Lambert T, Piergentili C, Bird JE, Gallagher KJ, Cruickshank FL, James P, Zarazua-Arvizu E, Horsfall LE, Waldron KJ, Wilson MD, Mackay CL, Basle A, Clarke DJ, Marles-Wright J
Sci Adv (2022) 8 pp. eabj4461-eabj4461 [ DOI: doi:10.1126/sciadv.abj4461 Pubmed: 35080974 ]
- Water (18 Da, Ligand)
- Linocin_m18 bacteriocin protein (28 kDa, Protein from Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2))
- Ternary complex of haliangium ochraceum encapsulin and encapsulated ferritin proteins (1 MDa, Complex from Haliangium ochraceum)
- Haliangium ochraceum encapsulated ferritin localisation sequence (14 kDa, Protein from Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2))
Resolution: 2.64 Å
EM Method: Single-particle
Fitted PDBs: 7oeu
Q-score: 0.596
Ross J, McIver Z, Lambert T, Piergentili C, Bird JE, Gallagher KJ, Cruickshank FL, James P, Zarazua-Arvizu E, Horsfall LE, Waldron KJ, Wilson MD, Mackay CL, Basle A, Clarke DJ, Marles-Wright J
Sci Adv (2022) 8 pp. eabj4461-eabj4461 [ DOI: doi:10.1126/sciadv.abj4461 Pubmed: 35080974 ]
- Haliangium ochraceum encapsulated ferritin (14 kDa, Protein from Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2))
- Linocin_m18 bacteriocin protein (28 kDa, Protein from Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2))
- Ternary complex of haliangium ochraceum encapsulin and encapsulated ferritin proteins (1 MDa, Complex from Haliangium ochraceum)