Ross J, McIver Z, Lambert T, Piergentili C, Bird JE, Gallagher KJ, Cruickshank FL, James P, Zarazua-Arvizu E, Horsfall LE, Waldron KJ, Wilson MD, Mackay CL, Basle A, Clarke DJ, Marles-Wright J
Sci Adv (2022) 8 pp. eabj4461-eabj4461 [ DOI: doi:10.1126/sciadv.abj4461 Pubmed: 35080974 ]
- Haliangium ochraceum encapsulated ferritin (Protein from Haliangium ochraceum)
- Encapsulin nano compartment (Complex from Haliangium ochraceum)
- Ternary complex of haliangium ochraceum encapsulin and encapsulated ferritin proteins (1 MDa, Complex from Haliangium ochraceum)
- Haliangium ochraceum encapsulin (Protein from Haliangium ochraceum)
Icosahedral cryo-EM reconstruction of Haliangium ochraceum encapsulin
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7odw
Q-score: 0.564
Ross J, McIver Z, Lambert T, Piergentili C, Bird JE, Gallagher KJ, Cruickshank FL, James P, Zarazua-Arvizu E, Horsfall LE, Waldron KJ, Wilson MD, Mackay CL, Basle A, Clarke DJ, Marles-Wright J
Sci Adv (2022) 8 pp. eabj4461-eabj4461 [ Pubmed: 35080974 DOI: doi:10.1126/sciadv.abj4461 ]
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7oe2
Q-score: 0.617
Ross J, McIver Z, Lambert T, Piergentili C, Bird JE, Gallagher KJ, Cruickshank FL, James P, Zarazua-Arvizu E, Horsfall LE, Waldron KJ, Wilson MD, Mackay CL, Basle A, Clarke DJ, Marles-Wright J
Sci Adv (2022) 8 pp. eabj4461-eabj4461 [ DOI: doi:10.1126/sciadv.abj4461 Pubmed: 35080974 ]
- Water (18 Da, Ligand)
- Linocin_m18 bacteriocin protein (28 kDa, Protein from Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2))
- Ternary complex of haliangium ochraceum encapsulin and encapsulated ferritin proteins (1 MDa, Complex from Haliangium ochraceum)
- Haliangium ochraceum encapsulated ferritin localisation sequence (14 kDa, Protein from Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2))
Resolution: 2.64 Å
EM Method: Single-particle
Fitted PDBs: 7oeu
Q-score: 0.596
Ross J, McIver Z, Lambert T, Piergentili C, Bird JE, Gallagher KJ, Cruickshank FL, James P, Zarazua-Arvizu E, Horsfall LE, Waldron KJ, Wilson MD, Mackay CL, Basle A, Clarke DJ, Marles-Wright J
Sci Adv (2022) 8 pp. eabj4461-eabj4461 [ Pubmed: 35080974 DOI: doi:10.1126/sciadv.abj4461 ]
- Ternary complex of haliangium ochraceum encapsulin and encapsulated ferritin proteins (1 MDa, Complex from Haliangium ochraceum)
- Haliangium ochraceum encapsulated ferritin (14 kDa, Protein from Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2))
- Linocin_m18 bacteriocin protein (28 kDa, Protein from Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2))
Ross J, McIver Z, Lambert T, Piergentili C, Bird JE, Gallagher KJ, Cruickshank FL, James P, Zarazua-Arvizu E, Horsfall LE, Waldron KJ, Wilson MD, Mackay CL, Basle A, Clarke DJ, Marles-Wright J
Sci Adv (2022) 8 pp. eabj4461-eabj4461 [ Pubmed: 35080974 DOI: doi:10.1126/sciadv.abj4461 ]
Open-open state of the Bt1762-Bt1763 levan transport system
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6zlt
Q-score: 0.451
Gray DA, White JBR, Oluwole AO, Rath P, Glenwright AJ, Mazur A, Zahn M, Basle A, Morland C, Evans SL, Cartmell A, Robinson CV, Hiller S, Ranson NA, Bolam DN, van den Berg B
Nat Commun (2021) 12 pp. 44-44 [ DOI: doi:10.1038/s41467-020-20285-y Pubmed: 33398001 ]
- Susc homolog (103 kDa, Protein from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482))
- Susd homolog (Complex from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482))
- Susc homolog (Complex from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482))
- Susd homolog (64 kDa, Protein from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482))
- Dimeric bt1762-bt1763 levan transporter complex (360 kDa, Complex)
Closed-closed state of the Bt1762-Bt1763 levan transport system
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6zlu
Q-score: 0.2
Gray DA, White JBR, Oluwole AO, Rath P, Glenwright AJ, Mazur A, Zahn M, Basle A, Morland C, Evans SL, Cartmell A, Robinson CV, Hiller S, Ranson NA, Bolam DN, van den Berg B
Nat Commun (2021) 12 pp. 44-44 [ DOI: doi:10.1038/s41467-020-20285-y Pubmed: 33398001 ]
- Susc homolog (115 kDa, Protein from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482))
- Susd homolog (66 kDa, Protein from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482))
- Susd homolog (Complex from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482))
- Susc homolog (Complex from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482))
- Dimeric bt1762-bt1763 levan transporter complex (360 kDa, Complex)
Open-closed state of the Bt1762-Bt1763 levan transport system
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 6zm1
Q-score: 0.242
Gray DA, White JBR, Oluwole AO, Rath P, Glenwright AJ, Mazur A, Zahn M, Basle A, Morland C, Evans SL, Cartmell A, Robinson CV, Hiller S, Ranson NA, Bolam DN, van den Berg B
Nat Commun (2021) 12 pp. 44-44 [ DOI: doi:10.1038/s41467-020-20285-y Pubmed: 33398001 ]
- Susc homolog (Complex from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482))
- Susd homolog (66 kDa, Protein from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482))
- Susd homolog (Complex from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482))
- Susc homolog (115 kDa, Protein from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482))
- Dimeric bt1762-bt1763 levan transporter complex (360 kDa, Complex)
Structure of the RagAB peptide importer in the 'closed-closed' state
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6sm3
Q-score: 0.585
Madej M, White JBR, Nowakowska Z, Rawson S, Scavenius C, Enghild JJ, Bereta GP, Pothula K, Kleinekathoefer U, Basle A, Ranson NA, Potempa J, van den Berg B
Nat Microbiol (2020) 5 pp. 1016-1025 [ Pubmed: 32393857 DOI: doi:10.1038/s41564-020-0716-y ]
- (1r,4s,6r)-6-({[2-(acetylamino)-2-deoxy-alpha-d-glucopyranosyl]oxy}methyl)-4-hydroxy-1-{[(15-methylhexadecanoyl)oxy]methyl}-4-oxido-7-oxo-3,5-dioxa-8-aza-4-phosphaheptacos-1-yl 15-methylhexadecanoate (1 kDa, Ligand)
- Gly-gly-ala-thr-thr-ala-thr-thr-thr-thr-ser-thr-ser (1 kDa, Protein from Porphyromonas gingivalis W83)
- Raga protein (100 kDa, Protein from Porphyromonas gingivalis (strain ATCC BAA-308 / W83))
- Palmitic acid (256 Da, Ligand)
- Lipoprotein ragb (54 kDa, Protein from Porphyromonas gingivalis (strain ATCC BAA-308 / W83))
- Ragab with putative peptide substrate (Complex from Porphyromonas gingivalis W83)
Structure of the RagAB peptide importer in the 'open-open' state
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6sml
Q-score: 0.559
Madej M, White JBR, Nowakowska Z, Rawson S, Scavenius C, Enghild JJ, Bereta GP, Pothula K, Kleinekathoefer U, Basle A, Ranson NA, Potempa J, van den Berg B
Nat Microbiol (2020) 5 pp. 1016-1025 [ Pubmed: 32393857 DOI: doi:10.1038/s41564-020-0716-y ]
- Ragab with putative peptide substrate (Complex from Porphyromonas gingivalis W83)
- Palmitic acid (256 Da, Ligand)
- Lipoprotein ragb (54 kDa, Protein from Porphyromonas gingivalis W83)
- (1r,4s,6r)-6-({[2-(acetylamino)-2-deoxy-alpha-d-glucopyranosyl]oxy}methyl)-4-hydroxy-1-{[(15-methylhexadecanoyl)oxy]methyl}-4-oxido-7-oxo-3,5-dioxa-8-aza-4-phosphaheptacos-1-yl 15-methylhexadecanoate (1 kDa, Ligand)
- Raga protein (101 kDa, Protein from Porphyromonas gingivalis W83)
- Gly-thr-gly-gly-ser-thr-gly-thr-thr-ser-ala-gly (952 Da, Protein from Porphyromonas gingivalis W83)