Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 6uus
Q-score: 0.535
Liang YL, Belousoff MJ, Fletcher MM, Zhang X, Khoshouei M, Deganutti G, Koole C, Furness SGB, Miller LJ, Hay DL, Christopoulos A, Reynolds CA, Danev R, Wootten D, Sexton PM
Acs Pharmacol Transl Sci (2020) 3 pp. 263-284 [ Pubmed: 32296767 DOI: doi:10.1021/acsptsci.9b00080 ]
- Calcitonin gene-related peptide type 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Adm (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, receptor activity-modifying protein 3, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, calcitonin gene-related peptide type 1 receptor (Complex from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Adm (6 kDa, Protein from Homo sapiens)
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Am2:clr:ramp3:gasdn:gb1:gg2:nb35 gpcr complex (Complex)
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 6uva
Q-score: 0.553
Liang YL, Belousoff MJ, Fletcher MM, Zhang X, Khoshouei M, Deganutti G, Koole C, Furness SGB, Miller LJ, Hay DL, Christopoulos A, Reynolds CA, Danev R, Wootten D, Sexton PM
Acs Pharmacol Transl Sci (2020) 3 pp. 263-284 [ Pubmed: 32296767 DOI: doi:10.1021/acsptsci.9b00080 ]
- Protein adm2 (5 kDa, Protein from Homo sapiens)
- Calcitonin gene-related peptide type 1 receptor (56 kDa, Protein from Homo sapiens)
- Am2:clr:ramp3:gasdn:gb1:gg2:nb35 gpcr complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, receptor activity-modifying protein 3, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, calcitonin gene-related peptide type 1 receptor (Complex from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- Protein adm2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6uun
Q-score: 0.48
Liang YL, Belousoff MJ, Fletcher MM, Zhang X, Khoshouei M, Deganutti G, Koole C, Furness SGB, Miller LJ, Hay DL, Christopoulos A, Reynolds CA, Danev R, Wootten D, Sexton PM
Acs Pharmacol Transl Sci (2020) 3 pp. 263-284 [ Pubmed: 32296767 DOI: doi:10.1021/acsptsci.9b00080 ]
- Calcitonin gene-related peptide type 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, receptor activity-modifying protein 2, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, calcitonin gene-related peptide type 1 receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Adm (Complex from Homo sapiens)
- Am:clr:ramp2:gasdn:gb1:gg2:nb35 gpcr complex (Complex)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Adm (6 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Receptor activity-modifying protein 2 (17 kDa, Protein from Homo sapiens)
Cryo-EM of GLP-1 receptor bound to TT-OAD2 non-peptidic agonist
Zhao P, Liang YL, Belousoff MJ, Deganutti G, Fletcher MM, Willard FS, Bell MG, Christe ME, Sloop KW, Inoue A, Truong TT, Clydesdale L, Furness SGB, Christopoulos A, Wang MW, Miller LJ, Reynolds CA, Danev R, Sexton PM, Wootten D
Nature (2020) 577 pp. 432-436 [ DOI: 10.1038/s41586-019-1902-z Pubmed: 31915381 ]
Image sets:
Non-peptide agonist (TT-OAD2) bound to the Glucagon-Like peptide-1 (GLP-1) Receptor
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6orv
Q-score: 0.538
Zhao P, Liang YL, Belousoff MJ, Deganutti G, Fletcher MM, Willard FS, Bell MG, Christe ME, Sloop KW, Inoue A, Truong TT, Clydesdale L, Furness SGB, Christopoulos A, Wang MW, Miller LJ, Reynolds CA, Danev R, Sexton PM, Wootten D
Nature (2020) 577 pp. 432-436 [ DOI: doi:10.1038/s41586-019-1902-z Pubmed: 31915381 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, glucagon-like peptide 1 receptor (Complex from Homo sapiens)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Homo sapiens)
- Tt-oad2:glp-1r:gs complex (120 kDa, Complex)
- N-{(3s,8s)-3-{4-[(3,4-dichlorophenyl)methoxy]phenyl}-7-[(1s)-1-phenylpropyl]-2,3,6,7,8,9-hexahydro[1,4]dioxino[2,3-g]isoquinoline-8-carbonyl}-4-(2,3-dimethylpyridin-4-yl)-l-phenylalanine (856 Da, Ligand)
- Nanobody 35 (15 kDa, Protein from Homo sapiens)