Cholecystokinin 1 receptor (CCK1R) Y140A mutant, Gq chimera (mGsqi) complex
Harikumar KG, Zhao P, Cary BP, Xu X, Desai AJ, Dong M, Mobbs JI, Toufaily C, Furness SGB, Christopoulos A, Belousoff MJ, Wootten D, Sexton PM, Miller LJ
PLoS Biol (2024) 22 pp. e3002673-e3002673 [ Pubmed: 39083706 DOI: doi:10.1371/journal.pbio.3002673 ]
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Complex of cck1r (y140a mutant) bound to minigs (gq, gi chimera), g-beta1, g-gamma-2 and cck8s. (150 kDa, Complex from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Amino group (16 Da, Ligand)
Cholecystokinin 1 receptor (CCK1R) sterol 7M mutant, Gq chimera (mGsqi) complex
Harikumar KG, Zhao P, Cary BP, Xu X, Desai AJ, Dong M, Mobbs JI, Toufaily C, Furness SGB, Christopoulos A, Belousoff MJ, Wootten D, Sexton PM, Miller LJ
PLoS Biol (2024) 22 pp. e3002673-e3002673 [ Pubmed: 39083706 DOI: doi:10.1371/journal.pbio.3002673 ]
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (29 kDa, Protein from Homo sapiens)
- Complex of cck1r (sterol 7m mutant) bound to minigs (gq, gi chimera), g-beta1, g-gamma-2 and cck8s. (150 kDa, Complex from Homo sapiens)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
Human Amylin1 Receptor in Complex with Gs and human Calcitonin Gene-Related Peptide
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 9auc
Q-score: 0.503
Cao J, Belousoff MJ, Danev R, Christopoulos A, Wootten D, Sexton PM
Biochemistry (2024) 63 pp. 1089-1096 [ Pubmed: 38603770 DOI: doi:10.1021/acs.biochem.4c00114 ]
- Human amylin 1 receptor in complex with gs and human calcitonin gene-related peptide (Complex from Homo sapiens)
- Receptor activity-modifying protein 1 (17 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcitonin gene-related peptide 1 (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
Resolution: 2.0 Å
EM Method: Single-particle
Fitted PDBs: 8f0j
Q-score: 0.621
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2024) 20 pp. 162-169 [ Pubmed: 37537379 DOI: doi:10.1038/s41589-023-01393-4 ]
- Calcitonin receptor in complex with gs protein and san45 peptide (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Water (18 Da, Ligand)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- (2s)-2-{[(1r)-1-hydroxyhexadecyl]oxy}-3-{[(1r)-1-hydroxyoctadecyl]oxy}propyl 2-(trimethylammonio)ethyl phosphate (766 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Phosphatidylethanolamine (734 Da, Ligand)
- Pramlintide analogue san45 (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- N-hexadecanoyl-l-glutamic acid (385 Da, Ligand)
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8f2a
Q-score: 0.556
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2024) 20 pp. 162-169 [ Pubmed: 37537379 DOI: doi:10.1038/s41589-023-01393-4 ]
- Pramlintide analogue san385 (3 kDa, Protein from Homo sapiens)
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- Human amylin 3 receptor in complex with gs protein and san385 peptide in another conformation (cluster 5) (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
Resolution: 2.0 Å
EM Method: Single-particle
Fitted PDBs: 8f2b
Q-score: 0.556
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2024) 20 pp. 162-169 [ Pubmed: 37537379 DOI: doi:10.1038/s41589-023-01393-4 ]
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Water (18 Da, Ligand)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- (2s)-2-{[(1r)-1-hydroxyhexadecyl]oxy}-3-{[(1r)-1-hydroxyoctadecyl]oxy}propyl 2-(trimethylammonio)ethyl phosphate (766 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Pramlintide analogue san45 (3 kDa, Protein from Homo sapiens)
- Amylin 3 receptor in complex with gs protein and san45 peptide (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- N-hexadecanoyl-l-glutamic acid (385 Da, Ligand)
Human PTH1R in complex with PTH(1-34) and Gs
Resolution: 2.55 Å
EM Method: Single-particle
Fitted PDBs: 8flq
Q-score: 0.538
Cary BP, Gerrard EJ, Belousoff MJ, Fletcher MM, Jiang Y, Russell IC, Piper SJ, Wootten D, Sexton PM
Structure (2023) 31 pp. 668-676.e5 [ DOI: doi:10.1016/j.str.2023.04.002 Pubmed: 37148874 ]
- Nanobody35 (13 kDa, Protein from Lama glama)
- Water (18 Da, Ligand)
- Parathyroid hormone (4 kDa, Protein from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (69 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Pth bound to the pth1r in complex with g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Human PTH1R in complex with PTHrP and Gs
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8flr
Q-score: 0.516
Cary BP, Gerrard EJ, Belousoff MJ, Fletcher MM, Jiang Y, Russell IC, Piper SJ, Wootten D, Sexton PM
Structure (2023) 31 pp. 668-676.e5 [ DOI: doi:10.1016/j.str.2023.04.002 Pubmed: 37148874 ]
- Nanobody35 (13 kDa, Protein from Lama glama)
- Pthrp[1-36] (4 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (69 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Pthrp bound to the pth1r in complex with g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Human PTH1R in complex with LA-PTH and Gs
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8flu
Q-score: 0.463
Cary BP, Gerrard EJ, Belousoff MJ, Fletcher MM, Jiang Y, Russell IC, Piper SJ, Wootten D, Sexton PM
Structure (2023) 31 pp. 668-676.e5 [ DOI: doi:10.1016/j.str.2023.04.002 Pubmed: 37148874 ]
- Long-acting pth (4 kDa, Protein from synthetic construct)
- Nanobody35 (13 kDa, Protein from Lama glama)
- Human pth1r in complex with la-pth and gs (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (69 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7ttw
Q-score: 0.598
Perlaza-Jimenez L, Tan KS, Piper SJ, Johnson RM, Bamert RS, Stubenrauch CJ, Wright A, Lupton D, Lithgow T, Belousoff MJ
Microbiol Spectr (2022) 10 pp. e0058322-e0058322 [ Pubmed: 35736238 DOI: doi:10.1128/spectrum.00583-22 ]
- 50s ribosomal protein l17 (13 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l23 (10 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l25 (23 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l20 (13 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l33 (5 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l4 (22 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l34 (5 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l36 (4 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l24 (11 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l19 (13 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l2 (30 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l18 (13 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l35 (7 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l22 (12 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal subunit from staphylococcus aureus - double mutation in ul3 leading to linezolid resistance (Complex from Staphylococcus aureus)
- 50s ribosomal protein l28 (6 kDa, Protein from Staphylococcus aureus)
- 5s rrna (36 kDa, RNA from Staphylococcus aureus)
- 50s ribosomal protein l32 (6 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l21 (11 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l30 (6 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l13 (16 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l3 (23 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l15 (15 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l27 (10 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l29 (8 kDa, Protein from Staphylococcus aureus)
- 23s rrna (946 kDa, RNA from Staphylococcus aureus)
- 50s ribosomal protein l16 (16 kDa, Protein from Staphylococcus aureus)
- 50s ribosomal protein l14 (13 kDa, Protein from Staphylococcus aureus)