Zhou M, Gamage ST, Tran KA, Bartee D, Wei X, Yin B, Berger S, Meier JL, Marmorstein R
bioRxiv (2024) [ DOI: doi:10.1101/2024.03.27.587050 Pubmed: 38585770 ]
- E.coli produced recombinant nat10 in complex with chemically synthetic cytidine-acetone-coa bisubstrate probe (247 MDa, Complex from Thermochaetoides thermophila DSM 1495)
- Rna cytidine acetyltransferase (121 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- [[(2~{r},3~{r},4~{r},5~{r})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{s})-4-[[3-[2-[3-[1-[(2~{r},3~{r},4~{r},5~{s})-5-(hydroxymethyl)-3,4-bis(oxidanyl)oxolan-2-yl]-2-oxidanylidene-pyrimidin-4-yl]sulfanyl-2-oxidanylidene-propyl]sulfanylethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate (1 kDa, Ligand)
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 9b0e
Q-score: 0.46
Zhou M, Gamage ST, Tran KA, Bartee D, Wei X, Yin B, Berger S, Meier JL, Marmorstein R
bioRxiv (2024) [ DOI: doi:10.1101/2024.03.27.587050 Pubmed: 38585770 ]
- E.coli produced recombinant nat10 in complex with adp and chemically synthetic cytidine-amide-coa bisubstrate probe (247 MDa, Complex from Thermochaetoides thermophila DSM 1495)
- [[(2~{r},3~{s},4~{s},5~{r})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{r})-4-[[3-[2-[2-[[1-[(2~{r},3~{s},4~{r},5~{r})-5-(hydroxymethyl)-3,4-bis(oxidanyl)oxolan-2-yl]-2-oxidanylidene-pyrimidin-4-yl]amino]-2-oxidanylidene-ethyl]sulfanylethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate (1 kDa, Ligand)
- Rna cytidine acetyltransferase (121 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 9b0i
Q-score: 0.531
Zhou M, Gamage ST, Tran KA, Bartee D, Wei X, Yin B, Berger S, Meier JL, Marmorstein R
bioRxiv (2024) [ DOI: doi:10.1101/2024.03.27.587050 Pubmed: 38585770 ]
- Magnesium ion (24 Da, Ligand)
- [[(2~{r},3~{s},4~{s},5~{r})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{r})-4-[[3-[2-[2-[[1-[(2~{r},3~{s},4~{r},5~{r})-5-(hydroxymethyl)-3,4-bis(oxidanyl)oxolan-2-yl]-2-oxidanylidene-pyrimidin-4-yl]amino]-2-oxidanylidene-ethyl]sulfanylethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate (1 kDa, Ligand)
- Sf9 produced recombinant nat10 in complex with adp/mg2+ and chemically synthetic cytidine-amide-coa bisubstrate probe (244 MDa, Complex from Thermochaetoides thermophila DSM 1495)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Rna cytidine acetyltransferase (121 kDa, Protein from Thermochaetoides thermophila DSM 1495)