Cryo-EM structure of the mammalian peptide transporter PepT2 bound to cefadroxil
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9bir
Q-score: 0.549
Parker JL, Deme JC, Lichtinger SM, Kuteyi G, Biggin PC, Lea SM, Newstead S
Nat Commun (2024) 15 pp. 8755-8755 [ DOI: doi:10.1038/s41467-024-53096-6 Pubmed: 39384780 ]
- Nanobody (Complex from Lama glama)
- Complex of the mammalian peptide transporter pept2 with nanobody and bound cefadroxil (Complex from Rattus norvegicus)
- Pept2 (Complex from Rattus norvegicus)
- Nanobody (14 kDa, Protein from Lama glama)
- Solute carrier family 15 member 2 (82 kDa, Protein from Rattus norvegicus)
- Cefadroxil (363 Da, Ligand)
Cryo-EM structure of the mammalian peptide transporter PepT2 bound to amoxicillin
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 9bis
Q-score: 0.523
Parker JL, Deme JC, Lichtinger SM, Kuteyi G, Biggin PC, Lea SM, Newstead S
Nat Commun (2024) 15 pp. 8755-8755 [ DOI: doi:10.1038/s41467-024-53096-6 Pubmed: 39384780 ]
- Nanobody (Complex from Lama glama)
- Complex of the mammalian peptide transporter pept2 with nanobody and bound amoxicillin (Complex from Rattus norvegicus)
- Pept2 (Complex from Rattus norvegicus)
- 2-{1-[2-amino-2-(4-hydroxy-phenyl)-acetylamino]-2-oxo-ethyl}-5,5-dimethyl-thiazolidine-4-carboxylic acid (367 Da, Ligand)
- Nanobody (14 kDa, Protein from Lama glama)
- Solute carrier family 15 member 2 (82 kDa, Protein from Rattus norvegicus)
Cryo-EM structure of the mammalian peptide transporter PepT2 bound to cloxacillin, pose 1
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9bit
Q-score: 0.539
Parker JL, Deme JC, Lichtinger SM, Kuteyi G, Biggin PC, Lea SM, Newstead S
Nat Commun (2024) 15 pp. 8755-8755 [ DOI: doi:10.1038/s41467-024-53096-6 Pubmed: 39384780 ]
- Complex of the mammalian peptide transporter pept2 with nanobody and bound cloxacillin, pose 1 (Complex from Rattus norvegicus)
- Nanobody (Complex from Lama glama)
- Cloxacillin (435 Da, Ligand)
- Pept2 (Complex from Rattus norvegicus)
- Nanobody (14 kDa, Protein from Lama glama)
- Solute carrier family 15 member 2 (82 kDa, Protein from Rattus norvegicus)
Cryo-EM structure of the mammalian peptide transporter PepT2 bound to cloxacillin, pose 2
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 9biu
Q-score: 0.548
Parker JL, Deme JC, Lichtinger SM, Kuteyi G, Biggin PC, Lea SM, Newstead S
Nat Commun (2024) 15 pp. 8755-8755 [ DOI: doi:10.1038/s41467-024-53096-6 Pubmed: 39384780 ]
- Nanobody (Complex from Lama glama)
- Cloxacillin (435 Da, Ligand)
- Pept2 (Complex from Rattus norvegicus)
- Nanobody (14 kDa, Protein from Lama glama)
- Solute carrier family 15 member 2 (82 kDa, Protein from Rattus norvegicus)
- Complex of the mammalian peptide transporter pept2 with nanobody and bound cloxacillin, pose 2 (Complex from Rattus norvegicus)
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 5.5
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8i47
Q-score: 0.519
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun (2024) 15 pp. 2967-2967 [ DOI: doi:10.1038/s41467-024-47370-w Pubmed: 38580666 ]
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Proton-gated ion channel (35 kDa, Protein from Gloeobacter violaceus)
- Water (18 Da, Ligand)
- Pentameric ligand-gated ion channel (Complex from Gloeobacter violaceus)
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in closed state
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 8i48
Q-score: 0.547
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun (2024) 15 pp. 2967-2967 [ DOI: doi:10.1038/s41467-024-47370-w Pubmed: 38580666 ]
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Pentameric ligand-gated ion channel (Complex from Gloeobacter violaceus)
- Proton-gated ion channel (35 kDa, Protein from Gloeobacter violaceus)
- Water (18 Da, Ligand)
- Chloride ion (35 Da, Ligand)
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 2.5
Resolution: 2.6476 Å
EM Method: Single-particle
Fitted PDBs: 8jj3
Q-score: 0.624
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun (2024) 15 pp. 2967-2967 [ DOI: doi:10.1038/s41467-024-47370-w Pubmed: 38580666 ]
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Pentameric ligand-gated ion channel (Complex from Gloeobacter violaceus)
- Proton-gated ion channel (35 kDa, Protein from Gloeobacter violaceus)
- Water (18 Da, Ligand)
- Chloride ion (35 Da, Ligand)
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in intermediate state
Resolution: 3.35 Å
EM Method: Single-particle
Fitted PDBs: 8wcq
Q-score: 0.378
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun (2024) 15 pp. 2967-2967 [ DOI: doi:10.1038/s41467-024-47370-w Pubmed: 38580666 ]
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Proton-gated ion channel (35 kDa, Protein from Gloeobacter violaceus)
- Chloride ion (35 Da, Ligand)
- Pentameric ligand-gated ion channel (Complex from Gloeobacter violaceus)
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in open state
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 8wcr
Q-score: 0.625
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun (2024) 15 pp. 2967-2967 [ DOI: doi:10.1038/s41467-024-47370-w Pubmed: 38580666 ]
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Pentameric ligand-gated ion channel (Complex from Gloeobacter violaceus)
- Proton-gated ion channel (35 kDa, Protein from Gloeobacter violaceus)
- Water (18 Da, Ligand)
- Chloride ion (35 Da, Ligand)
Cryo-EM structure of nanodisc (asolectin) reconstituted GLIC at pH 7.5
Resolution: 3.42 Å
EM Method: Single-particle
Fitted PDBs: 8i41
Q-score: 0.467
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun (2024) 15 pp. 2967-2967 [ DOI: doi:10.1038/s41467-024-47370-w Pubmed: 38580666 ]
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Proton-gated ion channel (35 kDa, Protein from Gloeobacter violaceus)
- Pentameric ligand-gated ion channel (Complex from Gloeobacter violaceus)