human Lig1-DNA-PCNA complex reconstituted in absence of ATP
Resolution: 4.58 Å
EM Method: Single-particle
Fitted PDBs: 7qnz
Q-score: 0.361
Blair K, Tehseen M, Raducanu VS, Shahid T, Lancey C, Rashid F, Creuhet R, Hamdan SM, De Biasio A
Nat Commun (2022) 13 pp. 7833-7833 [ Pubmed: 36539424 DOI: doi:10.1038/s41467-022-35475-z ]
- Dna ligase 1 (101 kDa, Protein from Homo sapiens)
- Adenosine monophosphate (347 Da, Ligand)
- Proliferating cell nuclear antigen (29 kDa, Protein from Homo sapiens)
- Oligo19ddc (5 kDa, DNA from synthetic construct)
- Oligo32 (9 kDa, DNA from synthetic construct)
- Human ligase holoenzyme without atp present (Complex from Homo sapiens)
- Oligo13p (3 kDa, DNA from synthetic construct)
complex of DNA ligase I and FEN1 on PCNA and DNA
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 7qo1
Q-score: 0.303
Blair K, Tehseen M, Raducanu VS, Shahid T, Lancey C, Rashid F, Creuhet R, Hamdan SM, De Biasio A
Nat Commun (2022) 13 pp. 7833-7833 [ Pubmed: 36539424 DOI: doi:10.1038/s41467-022-35475-z ]
- Complex of dna ligase i and fen1 on pcna and dna (Complex from Homo sapiens)
- Adenosine monophosphate (347 Da, Ligand)
- Proliferating cell nuclear antigen (29 kDa, Protein from Homo sapiens)
- Flap endonuclease 1 (42 kDa, Protein from Homo sapiens)
- Oligo19ddc (5 kDa, DNA from synthetic construct)
- Oligo32 (9 kDa, DNA from synthetic construct)
- Dna ligase 1 (84 kDa, Protein from Homo sapiens)
- Oligo13p (3 kDa, DNA from synthetic construct)
FEN1 holoenzyme with a nicked DNA substrate
Blair K, Tehseen M, Raducanu VS, Shahid T, Lancey C, Rashid F, Creuhet R, Hamdan SM, De Biasio A
Nat Commun (2022) 13 pp. 7833-7833 [ Pubmed: 36539424 DOI: doi:10.1038/s41467-022-35475-z ]
- Oligo19ddc, oligo13p and oligo32 (Complex from synthetic construct)
- Oligo13p (DNA from synthetic construct)
- Flap endonuclease i (Protein from Homo sapiens)
- Proliferating cell nuclear antigen and flap endonuclease i (Complex from Homo sapiens)
- Complex of fen1 with pcna and dna (Complex)
- Proliferating cell nuclear antigen (Protein from Homo sapiens)
- Oligo32 (DNA from synthetic construct)
- Oligo19ddc (DNA from synthetic construct)
Open conformation of the complex of DNA ligase I on PCNA and DNA in the presence of ATP
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 8b8t
Q-score: 0.42
Blair K, Tehseen M, Raducanu VS, Shahid T, Lancey C, Rashid F, Creuhet R, Hamdan SM, De Biasio A
Nat Commun (2022) 13 pp. 7833-7833 [ Pubmed: 36539424 DOI: doi:10.1038/s41467-022-35475-z ]
- Complex of dna ligase i on pcna and dna in the presence of atp (Complex from Homo sapiens)
- Dna ligase 1 (29 kDa, Protein from Homo sapiens)
- Proliferating cell nuclear antigen (28 kDa, Protein from Homo sapiens)
Cryo-EM multiframe micrographs of human Pol kappa complexed with DNA and wildtype PCNA
Lancey C, Tehseen M, Bakshi S, Percival M, Takahashi M, Sobhy MA, Raducanu VS, Blair K, Muskett FW, Ragan TJ, Crehuet R, Hamdan SM, De Biasio A
Nat Commun (2021) 12 [ Pubmed: 34667155 DOI: 10.1038/s41467-021-26251-6 ]
Image sets:
Cryo-EM multiframe micrographs of human Pol kappa complexed with DNA and monoubiquitylated PCNA
Lancey C, Tehseen M, Bakshi S, Percival M, Takahashi M, Sobhy MA, Raducanu VS, Blair K, Muskett FW, Ragan TJ, Crehuet R, Hamdan SM, De Biasio A
Nat Commun (2021) 12 [ Pubmed: 34667155 DOI: 10.1038/s41467-021-26251-6 ]
Image sets:
Human Pol Kappa holoenzyme with wt PCNA
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7nv0
Q-score: 0.473
Lancey C, Tehseen M, Bakshi S, Percival M, Takahashi M, Sobhy MA, Raducanu VS, Blair K, Muskett FW, Ragan TJ, Crehuet R, Hamdan SM, De Biasio A
Nat Commun (2021) 12 pp. 6095-6095 [ DOI: doi:10.1038/s41467-021-26251-6 Pubmed: 34667155 ]
- Pol kappa holoenzyme with wt-pcna (206 kDa, Complex)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Dna primer (7 kDa, DNA from synthetic construct)
- Proliferating cell nuclear antigen (29 kDa, Protein from Homo sapiens)
- Pol kappa holoenzyme (Complex from Homo sapiens)
- Dna (Complex from synthetic construct)
- Dna template (11 kDa, DNA from synthetic construct)
- Dna polymerase kappa (98 kDa, Protein from Homo sapiens)
Human Pol Kappa holoenzyme with Ub-PCNA
Resolution: 6.4 Å
EM Method: Single-particle
Fitted PDBs: 7nv1
Q-score: 0.188
Lancey C, Tehseen M, Bakshi S, Percival M, Takahashi M, Sobhy MA, Raducanu VS, Blair K, Muskett FW, Ragan TJ, Crehuet R, Hamdan SM, De Biasio A
Nat Commun (2021) 12 pp. 6095-6095 [ DOI: doi:10.1038/s41467-021-26251-6 Pubmed: 34667155 ]
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Pol kappa holoenzyme with ub-pcna (232 kDa, Complex)
- Dna primer (7 kDa, DNA from synthetic construct)
- Proliferating cell nuclear antigen (29 kDa, Protein from Homo sapiens)
- Pol kappa holoenzyme (Complex from Homo sapiens)
- Dna (Complex from synthetic construct)
- Dna template (11 kDa, DNA from synthetic construct)
- Dna polymerase kappa (98 kDa, Protein from Homo sapiens)