KZ52 Fab fragment bound to Ebola GP, subtomogram average from a tight mask
Hastie KM, Salie ZL, Ke Z, Halfmann PJ, DeWald LE, McArdle S, Grinyo A, Davidson E, Schendel SL, Hariharan C, Norris MJ, Yu X, Chennareddy C, Xiong X, Heinrich M, Holbrook MR, Doranz B, Crozier I, Kawaoka Y, Branco LM, Kuhn JH, Briggs JAG, Worwa G, Davis CW, Ahmed R, Saphire EO
Nat Commun (2025) 16 pp. 1293-1293 [ DOI: doi:10.1038/s41467-025-56452-2 Pubmed: 39900911 ]
KZ52 Fab fragment bound to Ebola GP, subtomogram average from a cylinder mask
Hastie KM, Salie ZL, Ke Z, Halfmann PJ, DeWald LE, McArdle S, Grinyo A, Davidson E, Schendel SL, Hariharan C, Norris MJ, Yu X, Chennareddy C, Xiong X, Heinrich M, Holbrook MR, Doranz B, Crozier I, Kawaoka Y, Branco LM, Kuhn JH, Briggs JAG, Worwa G, Davis CW, Ahmed R, Saphire EO
Nat Commun (2025) 16 pp. 1293-1293 [ DOI: doi:10.1038/s41467-025-56452-2 Pubmed: 39900911 ]
3A6 Fab fragment bound to Ebola GP, subtomogram average from a cylinder mask
Hastie KM, Salie ZL, Ke Z, Halfmann PJ, DeWald LE, McArdle S, Grinyo A, Davidson E, Schendel SL, Hariharan C, Norris MJ, Yu X, Chennareddy C, Xiong X, Heinrich M, Holbrook MR, Doranz B, Crozier I, Kawaoka Y, Branco LM, Kuhn JH, Briggs JAG, Worwa G, Davis CW, Ahmed R, Saphire EO
Nat Commun (2025) 16 pp. 1293-1293 [ DOI: doi:10.1038/s41467-025-56452-2 Pubmed: 39900911 ]
3A6 and KZ52 Fab fragments bound to Ebola GP, subtomogram average from a tight mask
Hastie KM, Salie ZL, Ke Z, Halfmann PJ, DeWald LE, McArdle S, Grinyo A, Davidson E, Schendel SL, Hariharan C, Norris MJ, Yu X, Chennareddy C, Xiong X, Heinrich M, Holbrook MR, Doranz B, Crozier I, Kawaoka Y, Branco LM, Kuhn JH, Briggs JAG, Worwa G, Davis CW, Ahmed R, Saphire EO
Nat Commun (2025) 16 pp. 1293-1293 [ DOI: doi:10.1038/s41467-025-56452-2 Pubmed: 39900911 ]
3A6 and KZ52 Fab fragments bound to Ebola GP, subtomogram average from a cylinder mask
Hastie KM, Salie ZL, Ke Z, Halfmann PJ, DeWald LE, McArdle S, Grinyo A, Davidson E, Schendel SL, Hariharan C, Norris MJ, Yu X, Chennareddy C, Xiong X, Heinrich M, Holbrook MR, Doranz B, Crozier I, Kawaoka Y, Branco LM, Kuhn JH, Briggs JAG, Worwa G, Davis CW, Ahmed R, Saphire EO
Nat Commun (2025) 16 pp. 1293-1293 [ DOI: doi:10.1038/s41467-025-56452-2 Pubmed: 39900911 ]
Native Lassa glycoprotein in complex with neutralizing antibodies 8.9F and 37.2D
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 7uot
Q-score: 0.559
Li H, Buck T, Zandonatti M, Yin J, Moon-Walker A, Fang J, Koval A, Heinrich ML, Rowland MM, Diaz Avalos R, Schendel SL, Parekh D, Zyla D, Enriquez A, Harkins S, Sullivan B, Smith V, Chukwudozie O, Watanabe R, Robinson JE, Garry RF, Branco LM, Hastie KM, Saphire EO
Sci Transl Med (2022) 14 pp. eabq0991-eabq0991 [ Pubmed: 36288283 DOI: doi:10.1126/scitranslmed.abq0991 ]
- Glycoprotein g1 (29 kDa, Protein from Lassa virus)
- 37.2d light chain (variable domain) (11 kDa, Protein from Homo sapiens)
- Glycoprotein g2 (26 kDa, Protein from Lassa virus)
- 8.9f heavy chain (variable domain) (15 kDa, Protein from Homo sapiens)
- 37.2d heavy chain (variable domain) (13 kDa, Protein from Homo sapiens)
- Native lassa glycoprotein in complex with 8.9f-scfv and 37.2d-scfv (Complex)
- 8.9f light chain (variable domain) (11 kDa, Protein from Homo sapiens)
Native Lassa glycoprotein in complex with neutralizing antibodies 12.1F and 37.2D
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 7uov
Q-score: 0.541
Li H, Buck T, Zandonatti M, Yin J, Moon-Walker A, Fang J, Koval A, Heinrich ML, Rowland MM, Diaz Avalos R, Schendel SL, Parekh D, Zyla D, Enriquez A, Harkins S, Sullivan B, Smith V, Chukwudozie O, Watanabe R, Robinson JE, Garry RF, Branco LM, Hastie KM, Saphire EO
Sci Transl Med (2022) 14 pp. eabq0991-eabq0991 [ Pubmed: 36288283 DOI: doi:10.1126/scitranslmed.abq0991 ]
- Glycoprotein g2 (26 kDa, Protein from Lassa virus)
- 37.2d light chain (variable domain) (11 kDa, Protein from Homo sapiens)
- 12.1f heavy chain (variable domain) (12 kDa, Protein from Homo sapiens)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus)
- 37.2d heavy chain (variable domain) (13 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 12.1f light chain (variable domain) (11 kDa, Protein from Homo sapiens)
- Native lassa glycoprotein in complex with 12.1f-scfv and 37.2d-scfv (Complex)
Structure of lineage I (Pinneo) Lassa virus glycoprotein bound to Fab 25.10C
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7uds
Q-score: 0.403
Buck TK, Enriquez AS, Schendel SL, Zandonatti MA, Harkins SS, Li H, Moon-Walker A, Robinson JE, Branco LM, Garry RF, Saphire EO, Hastie KM
Mbio (2022) 13 pp. e0127822-e0127822 [ DOI: doi:10.1128/mbio.01278-22 Pubmed: 35730904 ]
- Glycoprotein g2 (23 kDa, Protein from Lassa mammarenavirus)
- 25.10c fab heavy chain (24 kDa, Protein from Homo sapiens)
- 25.10c fab light chain (22 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Lineage i lassa virus glycoprotein bound to fab 25.10c (350 kDa, Complex)
- Glycoprotein g1 (28 kDa, Protein from Lassa mammarenavirus)
Lineage I (Pinneo) Lassa virus glycoprotein bound to 18.5C-M30 Fab
Resolution: 3.59 Å
EM Method: Single-particle
Fitted PDBs: 7ul7
Q-score: 0.336
Buck TK, Enriquez AS, Schendel SL, Zandonatti MA, Harkins SS, Li H, Moon-Walker A, Robinson JE, Branco LM, Garry RF, Saphire EO, Hastie KM
Mbio (2022) 13 pp. e0127822-e0127822 [ DOI: doi:10.1128/mbio.01278-22 Pubmed: 35730904 ]
- 18.5c-m30 fab heavy chain (26 kDa, Protein from Homo sapiens)
- Glycoprotein g1 (28 kDa, Protein from Lassa mammarenavirus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Lineage i (pinneo) lassa virus glycoprotein bound to 18.5c-m30 fab (350 kDa, Complex)
- Glycoprotein g2 (23 kDa, Protein from Lassa mammarenavirus)
- 18.5c-m30 fab light chain (25 kDa, Protein from Homo sapiens)
Structure of Lassa Virus glycoprotein (Josiah) bound to Fab 25.10C
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7tyv
Q-score: 0.447
Enriquez AS, Buck TK, Li H, Norris MJ, Moon-Walker A, Zandonatti MA, Harkins SS, Robinson JE, Branco LM, Garry RF, Saphire EO, Hastie KM
Cell Rep (2022) 39 pp. 110841-110841 [ DOI: doi:10.1016/j.celrep.2022.110841 Pubmed: 35613585 ]
- Glycoprotein g2 (19 kDa, Protein from Lassa virus)
- 25.10c fab heavy chain (24 kDa, Protein from Homo sapiens)
- Lassa virus glycoprotein josiah (Complex from Lassa virus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus)
- Lassa virus josiah gp complexed with anti-lassa virus antibody fab fragment 25.10c (350 kDa, Complex)
- 25.10c fab fragment (Complex from Homo sapiens)
- 25.10c fab light chain (25 kDa, Protein from Homo sapiens)