48-nm doublet microtubule from Tetrahymena thermophila strain MEC17
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8sf7
Q-score: 0.206
Yang SK, Kubo S, Black CS, Peri K, Dai D, Legal T, Valente-Paterno M, Gaertig J, Bui KH
eLife (2024) 12 [ Pubmed: 38598282 DOI: doi:10.7554/eLife.92219 ]
- Rib22 (22 kDa, Protein from Tetrahymena thermophila)
- Cfap127 (55 kDa, Protein from Tetrahymena thermophila)
- Cfap182b (11 kDa, Protein from Tetrahymena thermophila)
- Nucleoside diphosphate kinase (42 kDa, Protein from Tetrahymena thermophila)
- Cilia- and flagella-associated protein 45 (59 kDa, Protein from Tetrahymena thermophila)
- Oj3 (19 kDa, Protein from Tetrahymena thermophila)
- Cilia- and flagella-associated protein 52 (73 kDa, Protein from Tetrahymena thermophila)
- Stpg1a (42 kDa, Protein from Tetrahymena thermophila)
- Cfap77a (28 kDa, Protein from Tetrahymena thermophila)
- Stpg2 (33 kDa, Protein from Tetrahymena thermophila)
- Cfap21a (48 kDa, Protein from Tetrahymena thermophila)
- Cilia- and flagella-associated protein 53 (60 kDa, Protein from Tetrahymena thermophila)
- Rib26 (26 kDa, Protein from Tetrahymena thermophila)
- Cfap112b (32 kDa, Protein from Tetrahymena thermophila)
- Cfap161a (57 kDa, Protein from Tetrahymena thermophila)
- Cfam166a (25 kDa, Protein from Tetrahymena thermophila)
- Cfap115 (110 kDa, Protein from Tetrahymena thermophila)
- Parkin co-regulated protein pacrga (36 kDa, Protein from Tetrahymena thermophila)
- 48-nm repeat unit of microtubule doublet from tetrahymena thermophila strain mec17 (7 MDa, Cellular component from Tetrahymena thermophila)
- Cfap143 (30 kDa, Protein from Tetrahymena thermophila)
- Dna polymerase delta c4-type zinc-finger protein (31 kDa, Protein from Tetrahymena thermophila)
- Rib27b (26 kDa, Protein from Tetrahymena thermophila)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila)
- Protofilament ribbon protein (34 kDa, Protein from Tetrahymena thermophila)
- Cfap129 (31 kDa, Protein from Tetrahymena thermophila)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Rib43a protein (17 kDa, Protein from Tetrahymena thermophila)
- Cfam166c (25 kDa, Protein from Tetrahymena thermophila)
- Rib27a (27 kDa, Protein from Tetrahymena thermophila)
- Flagellar flij protein (12 kDa, Protein from Tetrahymena thermophila)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila)
- Flagellar microtugule protofilament ribbon protein (72 kDa, Protein from Tetrahymena thermophila)
- Nebulin (61 kDa, Protein from Tetrahymena thermophila)
- Rib57 (57 kDa, Protein from Tetrahymena thermophila)
- Cfap112a (30 kDa, Protein from Tetrahymena thermophila)
- Oj2 (19 kDa, Protein from Tetrahymena thermophila)
- Rib72b (60 kDa, Protein from Tetrahymena thermophila)
- Cfap182a (20 kDa, Protein from Tetrahymena thermophila)
- Cfap107 (21 kDa, Protein from Tetrahymena thermophila)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Sb1 (17 kDa, Protein from Tetrahymena thermophila)
- Cfam166b (17 kDa, Protein from Tetrahymena thermophila)
- Parkin co-regulated protein pacrgb (28 kDa, Protein from Tetrahymena thermophila)
- Cfap210 (61 kDa, Protein from Tetrahymena thermophila)
- Rib35 (34 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Ij34 (34 kDa, Protein from Tetrahymena thermophila)
- Rib38 (38 kDa, Protein from Tetrahymena thermophila)
- Cfap20 (22 kDa, Protein from Tetrahymena thermophila)
The structure of the Tetrahymena thermophila outer dynein arm on doublet microtubule
Resolution: 8.0 Å
EM Method: Single-particle
Fitted PDBs: 7moq
Q-score: 0.067
Kubo S, Yang SK, Black CS, Dai D, Valente-Paterno M, Gaertig J, Ichikawa M, Bui KH
EMBO Rep (2021) 22 pp. e52911-e52911 [ Pubmed: 34338432 DOI: doi:10.15252/embr.202152911 ]
- Outer dynein arm complex from tetrahymena thermophila (2 MDa, Cellular component from Tetrahymena thermophila CU428)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila CU428)
- Docking complex 1/2 protein (11 kDa, Protein from Tetrahymena thermophila CU428)
- Thioredoxin (12 kDa, Protein from Tetrahymena thermophila CU428)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein-1-alpha heavy chain, flagellar inner arm i1 complex protein, putative (475 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm docking complex protein oda protein (63 kDa, Protein from Tetrahymena thermophila CU428)
- Docking complex 1 protein (64 kDa, Protein from Tetrahymena thermophila CU428)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila CU428)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Dynein light chain (13 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain roadblock-type 2 protein (14 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein intermediate chain 2 (77 kDa, Protein from Tetrahymena thermophila CU428)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain roadblock-type 2 protein (18 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Outer arm dynein beta heavy chain (530 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Tetrahymena thermophila CU428)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dynein light chain 2a (15 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm docking complex protein oda protein (35 kDa, Protein from Tetrahymena thermophila CU428)
The inner junction complex of Chlamydomonas reinhardtii doublet microtubule
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6ve7
Q-score: 0.455
Khalifa AAZ, Ichikawa M, Dai D, Kubo S, Black C, Peri K, McAlear TS, Veyron S, Yang SK, Vargas J, Bechstedt S, Trempe JF, Bui KH
eLife (2020) 9 [ DOI: doi:10.7554/eLife.52760 Pubmed: 31951202 ]
- Pacrg (34 kDa, Protein from Chlamydomonas reinhardtii)
- Fap276 (10 kDa, Protein from Chlamydomonas reinhardtii)
- Inner junction complex of the cilia (1 MDa, Cellular component from Chlamydomonas reinhardtii)
- Tubulin beta (49 kDa, Protein from Chlamydomonas reinhardtii)
- Cilia- and flagella-associated protein 20 (22 kDa, Protein from Chlamydomonas reinhardtii)
- Protein flattop homolog (15 kDa, Protein from Chlamydomonas reinhardtii)
- Tubulin alpha (49 kDa, Protein from Chlamydomonas reinhardtii)
- Fap52 (68 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar associated protein (27 kDa, Protein from Chlamydomonas reinhardtii)
48-nm repeat unit of the doublet microtubule from Chlamydomonas reinhardtii
Resolution: 4.57 Å
EM Method: Single-particle
Fitted PDBs: 6ve7
Q-score: 0.028
Khalifa AAZ, Ichikawa M, Dai D, Kubo S, Black C, Peri K, McAlear TS, Veyron S, Yang SK, Vargas J, Bechstedt S, Trempe JF, Bui KH
eLife (2020) 9 [ DOI: doi:10.7554/eLife.52760 Pubmed: 31951202 ]
16-nm repeat of the doublet microtubule from Tetrahymena thermophila
Khalifa AAZ, Ichikawa M, Dai D, Kubo S, Black C, Peri K, McAlear TS, Veyron S, Yang SK, Vargas J, Bechstedt S, Trempe JF, Bui KH
eLife (2020) 9 [ DOI: doi:10.7554/eLife.52760 Pubmed: 31951202 ]
Cryo-EM structure of the 48-nm repeat unit of the doublet microtubule from Tetrahymena thermophila
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6u0h
Q-score: 0.357
Ichikawa M, Khalifa AAZ, Kubo S, Dai D, Basu K, Maghrebi MAF, Vargas J, Bui KH
PNAS (2019) 116 pp. 19930-19938 [ DOI: doi:10.1073/pnas.1911119116 Pubmed: 31527277 ]
- 48nm-repeat of microtubule doublet (Cellular component from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila)
- Tubulin lattice (Complex from Tetrahymena thermophila)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila)
Cryo-EM map of the A-tubule prepared by sonication
Ichikawa M, Khalifa AAZ, Kubo S, Dai D, Basu K, Maghrebi MAF, Vargas J, Bui KH
PNAS (2019) 116 pp. 19930-19938 [ DOI: doi:10.1073/pnas.1911119116 Pubmed: 31527277 ]
Cryo-EM map of the A-tubule prepared by 0.2% Sarkosyl treatment
Ichikawa M, Khalifa AAZ, Kubo S, Dai D, Basu K, Maghrebi MAF, Vargas J, Bui KH
PNAS (2019) 116 pp. 19930-19938 [ DOI: doi:10.1073/pnas.1911119116 Pubmed: 31527277 ]