The structure of the Tetrahymena thermophila outer dynein arm on doublet microtubule
Resolution: 8.0 Å
EM Method: Single-particle
Fitted PDBs: 7moq
Q-score: 0.067
Kubo S, Yang SK, Black CS, Dai D, Valente-Paterno M, Gaertig J, Ichikawa M, Bui KH
EMBO Rep (2021) 22 pp. e52911-e52911 [ DOI: doi:10.15252/embr.202152911 Pubmed: 34338432 ]
- Docking complex 1 protein (64 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Outer dynein arm complex from tetrahymena thermophila (2 MDa, Cellular component from Tetrahymena thermophila CU428)
- Dynein light chain roadblock-type 2 protein (14 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain roadblock-type 2 protein (18 kDa, Protein from Tetrahymena thermophila CU428)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Thioredoxin (12 kDa, Protein from Tetrahymena thermophila CU428)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Outer arm dynein beta heavy chain (530 kDa, Protein from Tetrahymena thermophila CU428)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynein-1-alpha heavy chain, flagellar inner arm i1 complex protein, putative (475 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein intermediate chain 2 (77 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm docking complex protein oda protein (63 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm docking complex protein oda protein (35 kDa, Protein from Tetrahymena thermophila CU428)
- Magnesium ion (24 Da, Ligand)
- Dynein light chain (13 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Docking complex 1/2 protein (11 kDa, Protein from Tetrahymena thermophila CU428)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain 2a (15 kDa, Protein from Tetrahymena thermophila CU428)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila CU428)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila CU428)
Cryo-EM structure of the 48-nm repeat unit of the doublet microtubule from Tetrahymena thermophila
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6u0h
Q-score: 0.357
Ichikawa M, Khalifa AAZ, Kubo S, Dai D, Basu K, Maghrebi MAF, Vargas J, Bui KH
PNAS (2019) 116 pp. 19930-19938 [ DOI: doi:10.1073/pnas.1911119116 Pubmed: 31527277 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Tubulin lattice (Complex from Tetrahymena thermophila)
- 48nm-repeat of microtubule doublet (Cellular component from Tetrahymena thermophila)
Cryo-EM structure of 8nm repeat tubulin lattice of the ciliary microtubule doublet
Resolution: 5.7 Å
EM Method: Single-particle
Fitted PDBs: 5ubq
Q-score: 0.144
Ichikawa M, Liu D, Kastritis PL, Basu K, Hsu TC, Yang S, Bui KH
Nat Commun (2017) 8 pp. 15035-15035 [ DOI: doi:10.1038/ncomms15035 Pubmed: 28462916 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- 8nm-repeat tubulin lattice of microtubule doublet (Cellular component from Tetrahymena thermophila)
- Tubulin beta chain (48 kDa, Protein from Tetrahymena thermophila)
Cryo-EM map of protofilament of microtubule doublet
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 5ucy
Q-score: 0.264
Ichikawa M, Liu D, Kastritis PL, Basu K, Hsu TC, Yang S, Bui KH
Nat Commun (2017) 8 pp. 15035-15035 [ DOI: doi:10.1038/ncomms15035 Pubmed: 28462916 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Tetrahymena thermophila)
- Protofilament from ciliary microtubule doublet (Cellular component from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (48 kDa, Protein from Tetrahymena thermophila)