The inner junction complex of Chlamydomonas reinhardtii doublet microtubule
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6ve7
Q-score: 0.455
Khalifa AAZ, Ichikawa M, Dai D, Kubo S, Black C, Peri K, McAlear TS, Veyron S, Yang SK, Vargas J, Bechstedt S, Trempe JF, Bui KH
eLife (2020) 9 [ Pubmed: 31951202 DOI: doi:10.7554/eLife.52760 ]
- Inner junction complex of the cilia (1 MDa, Cellular component from Chlamydomonas reinhardtii)
- Fap276 (10 kDa, Protein from Chlamydomonas reinhardtii)
- Tubulin alpha (49 kDa, Protein from Chlamydomonas reinhardtii)
- Pacrg (34 kDa, Protein from Chlamydomonas reinhardtii)
- Cilia- and flagella-associated protein 20 (22 kDa, Protein from Chlamydomonas reinhardtii)
- Fap52 (68 kDa, Protein from Chlamydomonas reinhardtii)
- Protein flattop homolog (15 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar associated protein (27 kDa, Protein from Chlamydomonas reinhardtii)
- Tubulin beta (49 kDa, Protein from Chlamydomonas reinhardtii)
48-nm repeat unit of the doublet microtubule from Chlamydomonas reinhardtii
Resolution: 4.57 Å
EM Method: Single-particle
Fitted PDBs: 6ve7
Q-score: 0.028
Khalifa AAZ, Ichikawa M, Dai D, Kubo S, Black C, Peri K, McAlear TS, Veyron S, Yang SK, Vargas J, Bechstedt S, Trempe JF, Bui KH
eLife (2020) 9 [ Pubmed: 31951202 DOI: doi:10.7554/eLife.52760 ]
16-nm repeat of the doublet microtubule from Tetrahymena thermophila
Khalifa AAZ, Ichikawa M, Dai D, Kubo S, Black C, Peri K, McAlear TS, Veyron S, Yang SK, Vargas J, Bechstedt S, Trempe JF, Bui KH
eLife (2020) 9 [ Pubmed: 31951202 DOI: doi:10.7554/eLife.52760 ]
Cryo-EM structure of the 48-nm repeat unit of the doublet microtubule from Tetrahymena thermophila
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6u0h
Q-score: 0.357
Ichikawa M, Khalifa AAZ, Kubo S, Dai D, Basu K, Maghrebi MAF, Vargas J, Bui KH
PNAS (2019) 116 pp. 19930-19938 [ DOI: doi:10.1073/pnas.1911119116 Pubmed: 31527277 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Tubulin lattice (Complex from Tetrahymena thermophila)
- 48nm-repeat of microtubule doublet (Cellular component from Tetrahymena thermophila)
Cryo-EM map of the A-tubule prepared by sonication
Ichikawa M, Khalifa AAZ, Kubo S, Dai D, Basu K, Maghrebi MAF, Vargas J, Bui KH
PNAS (2019) 116 pp. 19930-19938 [ DOI: doi:10.1073/pnas.1911119116 Pubmed: 31527277 ]
Cryo-EM map of the A-tubule prepared by 0.2% Sarkosyl treatment
Ichikawa M, Khalifa AAZ, Kubo S, Dai D, Basu K, Maghrebi MAF, Vargas J, Bui KH
PNAS (2019) 116 pp. 19930-19938 [ DOI: doi:10.1073/pnas.1911119116 Pubmed: 31527277 ]