Single particle cryo-EM of SARS-CoV-2 Spike protein in complex with the single chain fragment scFv76
Milazzo FM, Chaves-Sanjuan A, Minenkova O, Santapaola D, Anastasi AM, Battistuzzi G, Chiapparino C, Rosi A, Merlo Pich E, Albertoni C, Marra E, Luberto L, Viollet C, Spagnoli LG, Riccio A, Rossi A, Santoro MG, Ballabio F, Paissoni C, Camilloni C, Bolognesi M, De Santis R
Molecular therapy : the journal of the American Society of Gene Therapy (2022) pp. S1525-0016(22)00564 [ Pubmed: 36114671 DOI: 10.1016/j.ymthe.2022.09.010 ]
Image sets:
SARS-CoV-2 Spike protein in complex with the single chain fragment scFv76
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7zce
Q-score: 0.354
Milazzo FM, Chaves-Sanjuan A, Minenkova O, Santapaola D, Anastasi AM, Battistuzzi G, Chiapparino C, Rosi A, Merlo Pich E, Albertoni C, Marra E, Luberto L, Viollet C, Spagnoli LG, Riccio A, Rossi A, Santoro MG, Ballabio F, Paissoni C, Camilloni C, Bolognesi M, De Santis R
Mol Ther (2022) 31 pp. 362-373 [ DOI: doi:10.1016/j.ymthe.2022.09.010 Pubmed: 36114671 ]
- Spike glycoprotein,fibritin (142 kDa, Protein from Enterobacteria phage T4)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Scfv76 single chain fragment (28 kDa, Protein from Homo sapiens)
- Sars-cov-2 spike protein (Complex from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 spike protein in complex with the single chain fragment scfv76 (510 kDa, Complex)
- Scfv76 single chain fragment (Complex from Homo sapiens)
SARS-CoV-2 Spike RBD in complex with the single chain fragment scFv76 (Focused Refinement)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7zcf
Q-score: 0.387
Milazzo FM, Chaves-Sanjuan A, Minenkova O, Santapaola D, Anastasi AM, Battistuzzi G, Chiapparino C, Rosi A, Merlo Pich E, Albertoni C, Marra E, Luberto L, Viollet C, Spagnoli LG, Riccio A, Rossi A, Santoro MG, Ballabio F, Paissoni C, Camilloni C, Bolognesi M, De Santis R
Mol Ther (2022) 31 pp. 362-373 [ DOI: doi:10.1016/j.ymthe.2022.09.010 Pubmed: 36114671 ]
- Spike glycoprotein,fibritin (142 kDa, Protein from Enterobacteria phage T4)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Scfv76 single chain fragment (28 kDa, Protein from Homo sapiens)
- Sars-cov-2 spike protein (Complex from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 spike protein in complex with the single chain fragment scfv76 (510 kDa, Complex)
- Scfv76 single chain fragment (Complex from Homo sapiens)
Structure of Azospirillum brasilense Glutamate Synthase in a4b4 oligomeric state.
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6s6s
Q-score: 0.451
Swuec P, Chaves-Sanjuan A, Camilloni C, Vanoni MA, Bolognesi M
J Mol Biol (2019) 431 pp. 4523-4526 [ DOI: doi:10.1016/j.jmb.2019.08.011 Pubmed: 31473159 ]
- Flavin mononucleotide (456 Da, Ligand)
- Fe3-s4 cluster (295 Da, Ligand)
- Glutamate synthase [nadph] large chain (166 kDa, Protein from Azospirillum brasilense)
- Glutamate synthase complex in a4b4 oligomeric state (Complex from Azospirillum brasilense)
- Iron/sulfur cluster (351 Da, Ligand)
- Glutamate synthase [nadph] small chain (52 kDa, Protein from Azospirillum brasilense)
- Flavin-adenine dinucleotide (785 Da, Ligand)
Structure of Azospirillum brasilense Glutamate Synthase in a4b3 oligomeric state
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6s6t
Q-score: 0.432
Swuec P, Chaves-Sanjuan A, Camilloni C, Vanoni MA, Bolognesi M
J Mol Biol (2019) 431 pp. 4523-4526 [ DOI: doi:10.1016/j.jmb.2019.08.011 Pubmed: 31473159 ]
- Flavin mononucleotide (456 Da, Ligand)
- Fe3-s4 cluster (295 Da, Ligand)
- Glutamate synthase [nadph] large chain (166 kDa, Protein from Azospirillum brasilense)
- Iron/sulfur cluster (351 Da, Ligand)
- Glutamate synthase [nadph] small chain (52 kDa, Protein from Azospirillum brasilense)
- Flavin-adenine dinucleotide (785 Da, Ligand)
- Glutamate synthase complex in a4b3 oligomeric state (Complex from Azospirillum brasilense)
Structure of Azospirillum brasilense Glutamate Synthase in a6b4 oligomeric state.
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6s6u
Q-score: 0.501
Swuec P, Chaves-Sanjuan A, Camilloni C, Vanoni MA, Bolognesi M
J Mol Biol (2019) 431 pp. 4523-4526 [ DOI: doi:10.1016/j.jmb.2019.08.011 Pubmed: 31473159 ]
- Fe3-s4 cluster (295 Da, Ligand)
- Flavin mononucleotide (456 Da, Ligand)
- Glutamate synthase [nadph] small chain (52 kDa, Protein from Azospirillum brasilense)
- Glutamate synthase [nadph] large chain (166 kDa, Protein from Azospirillum brasilense)
- Iron/sulfur cluster (351 Da, Ligand)
- Flavin-adenine dinucleotide (785 Da, Ligand)
- Glutamate synthase complex in a6b4 oligomeric state (Complex from Azospirillum brasilense)
Structure of Azospirillum brasilense Glutamate Synthase in a6b6 oligomeric state.
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6s6x
Q-score: 0.485
Swuec P, Chaves-Sanjuan A, Camilloni C, Vanoni MA, Bolognesi M
J Mol Biol (2019) 431 pp. 4523-4526 [ DOI: doi:10.1016/j.jmb.2019.08.011 Pubmed: 31473159 ]
- Fe3-s4 cluster (295 Da, Ligand)
- Flavin mononucleotide (456 Da, Ligand)
- Glutamate synthase [nadph] small chain (52 kDa, Protein from Azospirillum brasilense)
- Glutamate synthase [nadph] large chain (166 kDa, Protein from Azospirillum brasilense)
- Flavin-adenine dinucleotide (785 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Glutamate synthase complex in a6b6 oligomeric state (Complex from Azospirillum brasilense)
Resolution: 4.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 6hud
Q-score: 0.436
Swuec P, Lavatelli F, Tasaki M, Paissoni C, Rognoni P, Maritan M, Brambilla F, Milani P, Mauri P, Camilloni C, Palladini G, Merlini G, Ricagno S, Bolognesi M
Nat Commun (2019) 10 pp. 1269-1269 [ Pubmed: 30894521 DOI: doi:10.1038/s41467-019-09133-w ]