Cryo-EM Structure of Wildtype Smooth Muscle Gamma Actin (ACTG2)
Resolution: 2.45 Å
EM Method: Helical reconstruction
Fitted PDBs: 8v2o
Q-score: 0.589
Ceron RH, Baez-Cruz FA, Palmer NJ, Carman PJ, Boczkowska M, Heuckeroth RO, Ostap EM, Dominguez R
Sci Adv (2024) 10 pp. eadn6615-eadn6615 [ Pubmed: 38820162 DOI: doi:10.1126/sciadv.adn6615 ]
- Actg2 filament (Complex from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Actin, gamma-enteric smooth muscle (41 kDa, Protein from Homo sapiens)
Cryo-EM Structure of Smooth Muscle Gamma Actin (ACTG2) Mutant R257C
Resolution: 2.72 Å
EM Method: Helical reconstruction
Fitted PDBs: 8v2z
Q-score: 0.571
Ceron RH, Baez-Cruz FA, Palmer NJ, Carman PJ, Boczkowska M, Heuckeroth RO, Ostap EM, Dominguez R
Sci Adv (2024) 10 pp. eadn6615-eadn6615 [ Pubmed: 38820162 DOI: doi:10.1126/sciadv.adn6615 ]
- Magnesium ion (24 Da, Ligand)
- Actin, gamma-enteric smooth muscle (41 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actg2 r257c filament (Complex from Homo sapiens)
Smooth Muscle Gamma Actin (ACTG2) Filament Mutant R40C
Resolution: 2.54 Å
EM Method: Helical reconstruction
Fitted PDBs: 8v30
Q-score: 0.585
Ceron RH, Baez-Cruz FA, Palmer NJ, Carman PJ, Boczkowska M, Heuckeroth RO, Ostap EM, Dominguez R
Sci Adv (2024) 10 pp. eadn6615-eadn6615 [ Pubmed: 38820162 DOI: doi:10.1126/sciadv.adn6615 ]
- Magnesium ion (24 Da, Ligand)
- Actin, gamma-enteric smooth muscle (41 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phalloidin (808 Da, Protein from synthetic construct)
- Actg2 r40c filament (Complex from Homo sapiens)
Cryo-EM structure of Cortactin-bound to Arp2/3 complex
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 8tah
Q-score: 0.514
Fregoso FE, Boczkowska M, Rebowski G, Carman PJ, van Eeuwen T, Dominguez R
Nat Commun (2023) 14 pp. 6894-6894 [ Pubmed: 37898612 DOI: doi:10.1038/s41467-023-42229-y ]
- Magnesium ion (24 Da, Ligand)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 2 (34 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 1a (41 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 3 (20 kDa, Protein from Bos taurus)
- Src substrate cortactin (8 kDa, Protein from Mus musculus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin-related protein 2 (44 kDa, Protein from Bos taurus)
- Actin-related protein 3 (47 kDa, Protein from Bos taurus)
- Cortactin nta bound to arp2/3 complex (223 kDa, Complex from Bos taurus)
Cryo-EM structure of F-actin in the ADP state
Resolution: 2.26 Å
EM Method: Helical reconstruction
Fitted PDBs: 8f8p
Q-score: 0.587
Carman PJ, Barrie KR, Rebowski G, Dominguez R
Science (2023) 380 pp. 1287-1292 [ DOI: doi:10.1126/science.adg6812 Pubmed: 37228182 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- F-actin in the adp state (Complex from Oryctolagus cuniculus)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
Cryo-EM structure of the CapZ-capped barbed end of F-actin
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8f8q
Q-score: 0.571
Carman PJ, Barrie KR, Rebowski G, Dominguez R
Science (2023) 380 pp. 1287-1292 [ DOI: doi:10.1126/science.adg6812 Pubmed: 37228182 ]
- F-actin-capping protein subunit alpha-1 (32 kDa, Protein from Homo sapiens)
- F-actin-capping protein subunit beta (31 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
- Capz (alpha and beta subunits) (Complex from Homo sapiens)
- Capz-capped barbed end of f-actin (Complex)
- Actin filament (Complex from Oryctolagus cuniculus)
Cryo-EM structure of the free barbed end of F-actin
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8f8r
Q-score: 0.469
Carman PJ, Barrie KR, Rebowski G, Dominguez R
Science (2023) 380 pp. 1287-1292 [ DOI: doi:10.1126/science.adg6812 Pubmed: 37228182 ]
- Free barbed end of f-actin (Complex from Oryctolagus cuniculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
Cryo-EM structure of the free pointed end of F-actin
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8f8s
Q-score: 0.572
Carman PJ, Barrie KR, Rebowski G, Dominguez R
Science (2023) 380 pp. 1287-1292 [ DOI: doi:10.1126/science.adg6812 Pubmed: 37228182 ]
- Free pointed end of f-actin (Complex from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Cryo-EM structure of the Tropomodulin-capped pointed end of F-actin
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 8f8t
Q-score: 0.462
Carman PJ, Barrie KR, Rebowski G, Dominguez R
Science (2023) 380 pp. 1287-1292 [ DOI: doi:10.1126/science.adg6812 Pubmed: 37228182 ]
- Tropomodulin (tmod) (Complex from Homo sapiens)
- Tropomodulin-1 (40 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Tropomodulin-capped pointed end of f-actin (Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
- Actin filament (Complex from Oryctolagus cuniculus)