Cryo-EM imaging scaffold subunits A and B used to display KRAS G12C complex with GDP
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8g3k
Q-score: 0.668
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ DOI: doi:10.1073/pnas.2305494120 Pubmed: 37669364 ]
KRAS G12C complex with GDP imaged on a cryo-EM imaging scaffold
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 8g42
Q-score: 0.504
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ DOI: doi:10.1073/pnas.2305494120 Pubmed: 37669364 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Rcg-33 - cryo-em imaging scaffold subunit b fused to darpin (35 kDa, Protein from synthetic construct)
- Gtpase kras (21 kDa, Protein from Homo sapiens)
- Kras g12c displayed on a cryo-em imaging scaffold (Complex from synthetic construct)
KRAS G12C complex with GDP and AMG 510 imaged on a cryo-EM imaging scaffold
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 8g47
Q-score: 0.465
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ DOI: doi:10.1073/pnas.2305494120 Pubmed: 37669364 ]
- Amg 510 (bound form) (562 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Kras g12c complex with gdp and amg 510 imaged on a cryo-em imaging scaffold (Complex from synthetic construct)
- Rcg-33 - cryo-em imaging scaffold subunit b fused to darpin (35 kDa, Protein from synthetic construct)
- Gtpase kras (21 kDa, Protein from Homo sapiens)
Green Fluorescence Protein imaged on a cryo-EM imaging scaffold
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8g4e
Q-score: 0.566
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ DOI: doi:10.1073/pnas.2305494120 Pubmed: 37669364 ]
KRAS G12V complex with GDP imaged on a cryo-EM imaging scaffold
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8g4f
Q-score: 0.546
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ DOI: doi:10.1073/pnas.2305494120 Pubmed: 37669364 ]
- Kras g12v complex with gdp displayed on a cryo-em imaging scaffold (Complex from synthetic construct)
- Gtpase kras (19 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Rcg-33 - cryo-em imaging scaffold subunit b fused to darpin (35 kDa, Protein from synthetic construct)
KRAS G13C complex with GDP imaged on a cryo-EM imaging scaffold
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8g4h
Q-score: 0.548
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ DOI: doi:10.1073/pnas.2305494120 Pubmed: 37669364 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Gtpase kras (19 kDa, Protein from Homo sapiens)
- Rcg-33 - cryo-em imaging scaffold subunit b fused to darpin (35 kDa, Protein from synthetic construct)
- Kras g13c complex with gdp displayed on a cryo-em imaging scaffold (Complex from synthetic construct)
Nudaurelia capensis omega virus procapsid at pH7.6 (insect cell expressed VLPs)
Castells-Graells R, Hesketh EL, Johnson JE, Ranson NA, Lawson DM, Lomonossoff GP
To Be Published
Image sets:
Castells-Graells R, Hesketh EL, Johnson JE, Ranson NA, Lawson DM, Lomonossoff GP
To Be Published
Image sets:
Castells-Graells R, Hesketh EL, Johnson JE, Ranson NA, Lawson DM, Lomonossoff GP
To Be Published
Image sets:
Castells-Graells R, Hesketh EL, Johnson JE, Ranson NA, Lawson DM, Lomonossoff GP
To Be Published
Image sets: