Cryo-EM structure of rat cardiac sodium channel NaV1.5 with batrachotoxin analog BTX-B
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8t6l
Q-score: 0.469
Tonggu L, Wisedchaisri G, Gamal El-Din TM, Lenaeus MJ, Logan MM, Toma T, Du Bois J, Zheng N, Catterall WA
Nat Commun (2024) 15 pp. 2306-2306 [ DOI: doi:10.1038/s41467-024-45958-w Pubmed: 38485923 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (3beta,14beta,17beta,25r)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en (544 Da, Ligand)
- Water (18 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Sodium channel nav1.5 and batrachotoxinin-a 20-alpha-benzoate (200 kDa, Cellular component from Rattus norvegicus)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- Sodium channel protein type 5 subunit alpha,green fluorescent protein (213 kDa, Protein from Aequorea victoria)
- (1r)-1-[(5ar,7ar,9r,11as,11bs,12r,13ar)-9,12-dihydroxy-2,11a-dimethyl-1,2,3,4,7a,8,9,10,11,11a,12,13-dodecahydro-7h-9,11b-epoxy-13a,5a-prop[1]enophenanthro[2,1-f][1,4]oxazepin-14-yl]ethyl benzoate (521 Da, Ligand)
Cardiac sodium channel in complex with LqhIII
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7xsu
Q-score: 0.433
To Be Published
- Beta-d-mannopyranose (180 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sodium channel protein type 5 subunit alpha,g protein/gfp fusion protein (209 kDa, Protein from Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP)
- (3beta,14beta,17beta,25r)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en (544 Da, Ligand)
- Alpha-like toxin lqh3 (7 kDa, Protein from Leiurus quinquestriatus hebraeus)
- Lqhiii (Complex)
- Cardiac sodium channel complexed with toxin lqhiii (300 kDa, Complex)
- Cardiac sodium channel with a gfp-flag tag fused at the c-terminus (Complex from Rattus norvegicus)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
Rat Cardiac Sodium Channel with Ranolazine Bound
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8f6p
Q-score: 0.512
Lenaeus M, Gamal El-Din TM, Tonggu L, Zheng N, Catterall WA
Nat Cardiovasc Res (2023) 2 pp. 587-594 [ Pubmed: 39185478 DOI: doi:10.1038/s44161-023-00271-5 ]
- Beta-d-mannopyranose (180 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sodium channel protein type 5 subunit alpha,green fluorescent protein (209 kDa, Protein from Aequorea victoria)
- Complex of rat cardiac sodium channel with the antiarrhythmic drug ranolazine (250 kDa, Complex from Rattus norvegicus)
- (r)-ranolazine (427 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7fbs
Q-score: 0.477
Jiang D, Banh R, Gamal El-Din TM, Tonggu L, Lenaeus MJ, Pomes R, Zheng N, Catterall WA
Cell (2021) 184 pp. 5151- [ DOI: doi:10.1016/j.cell.2021.08.021 Pubmed: 34520724 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (3beta,14beta,17beta,25r)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en (544 Da, Ligand)
- Sodium channel with four homologous domains (250 kDa, Complex from Rattus norvegicus)
- 1-[2-[(2r)-2-oxidanyl-3-(propylamino)propoxy]phenyl]-3-phenyl-propan-1-one (341 Da, Ligand)
- Sodium channel protein type 5 subunit alpha,sodium channel protein type 5 subunit alpha,sodium channel protein type 5 subunit alpha,g protein/gfp fusion protein (209 kDa, Protein from Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
Cardiac Sodium channel with toxin bound
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7k18
Q-score: 0.411
Jiang D, Tonggu L, Gamal El-Din TM, Banh R, Pomes R, Zheng N, Catterall WA
Nat Commun (2021) 12 pp. 128-128 [ Pubmed: 33397917 DOI: doi:10.1038/s41467-020-20078-3 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sodium channel protein type 5 subunit alpha, enhanced green fluorescent protein (209 kDa, Protein from Aequorea victoria)
- Sodium channel protein type 5 subunit alpha (Complex from Rattus norvegicus)
- Cryo-em map for sodium channel and scorpion toxin complex (250 kDa, Complex)
- Alpha-like toxin lqh3 (Complex from Leiurus hebraeus)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Alpha-like toxin lqh3 (7 kDa, Protein from Leiurus hebraeus)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7k48
Q-score: 0.34
Wisedchaisri G, Tonggu L, Gamal El-Din TM, McCord E, Zheng N, Catterall WA
Mol Cell (2021) 81 pp. 38-48.e4 [ Pubmed: 33232657 DOI: doi:10.1016/j.molcel.2020.10.039 ]
- M3-huwentoxin-iv (Complex from Haplopelma schmidti)
- Mu-theraphotoxin-hs2a (3 kDa, Protein from Haplopelma schmidti)
- Complex of navab/nav1.7-vs2a chimera and m3-huwentoxin-iv (300 kDa, Complex)
- Navab/nav1.7-vs2a chimera (Complex from Escherichia coli)
- Maltose/maltodextrin-binding periplasmic protein,ion transport protein,sodium channel protein type 9 subunit alpha chimera (68 kDa, Protein from Arcobacter butzleri (strain RM4018))
Cryo-EM structure of a de novo designed 16-helix transmembrane nanopore, TMHC8_R.
Resolution: 7.6 Å
EM Method: Single-particle
Fitted PDBs: 6u1s
Q-score: 0.11
Xu C, Lu P, Gamal El-Din TM, Pei X, Johnson MJ, Uyeda A, Bick MJ, Jiang D, Hsia Y, Brunette TJ, Ma D, Lynch E, Boyken SE, Huang P, Stewart L, Kollman JK, Luisi BF, Matsuura T, Catterall WA, Baker D
To Be Published
A de novo designed transmembrane nanopore, TMH4C4
Resolution: 5.9 Å
EM Method: Single-particle
Fitted PDBs: 6m6z
Q-score: 0.126
Xu C, Lu P, Gamal El-Din TM, Pei XY, Johnson MC, Uyeda A, Bick MJ, Xu Q, Jiang D, Bai H, Reggiano G, Hsia Y, Brunette TJ, Dou J, Ma D, Lynch EM, Boyken SE, Huang PS, Stewart L, DiMaio F, Kollman JM, Luisi BF, Matsuura T, Catterall WA, Baker D
Nature (2020) 585 pp. 129-134 [ DOI: doi:10.1038/s41586-020-2646-5 Pubmed: 32848250 ]
- Tmh4c4 (23 kDa, Protein from Escherichia coli)
- A de novo designed transmembrane nanopore (Complex from Escherichia coli)
Cardiac sodium channel with flecainide
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 6uz0
Q-score: 0.459
Jiang D, Shi H, Tonggu L, Gamal El-Din TM, Lenaeus MJ, Zhao Y, Yoshioka C, Zheng N, Catterall WA
Cell (2020) 180 pp. 122-134.e10 [ Pubmed: 31866066 DOI: doi:10.1016/j.cell.2019.11.041 ]
- Cardiac sodium channel complex with flecainide (250 kDa, Complex from Rattus norvegicus)
- (3beta,14beta,17beta,25r)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en (544 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Flecainide (414 Da, Ligand)
- Sodium channel protein type 5 subunit alpha,green fluorescent protein (209 kDa, Protein from Aequorea victoria)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6uz3
Q-score: 0.401
Jiang D, Shi H, Tonggu L, Gamal El-Din TM, Lenaeus MJ, Zhao Y, Yoshioka C, Zheng N, Catterall WA
Cell (2020) 180 pp. 122-134.e10 [ Pubmed: 31866066 DOI: doi:10.1016/j.cell.2019.11.041 ]
- (3beta,14beta,17beta,25r)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en (544 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sodium channel protein type 5 subunit alpha,green fluorescent protein (209 kDa, Protein from Aequorea victoria)
- Cardiac sodium channel apo structure (250 kDa, Complex from Rattus norvegicus)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)