TniQ-capped TnsC-ATP-dsDNA complex
Schmitz M, Querques I, Oberli S, Chanez C, Jinek M
Cell (2022) 185 pp. 4999-5010.e17 [ DOI: 10.1016/j.cell.2022.11.009 Pubmed: 36435179 ]
Image sets:
Cas12k-sgRNA-dsDNA-S15-TniQ-TnsC transposon recruitment complex
Schmitz M, Querques I, Oberli S, Chanez C, Jinek M
Cell (2022) 185 pp. 4999-5010.e17 [ DOI: 10.1016/j.cell.2022.11.009 Pubmed: 36435179 ]
Image sets:
ShCas12k in complex with a sgRNA and a dsDNA target
Querques I, Schmitz M, Oberli S, Chanez C, Jinek M
Nature (2021) 599 pp. 497-502 [ Pubmed: 34759315 DOI: 10.1038/s41586-021-04030-z ]
Image sets:
Scytonema hofmannii TnsC bound to AMPPNP and DNA
Querques I, Schmitz M, Oberli S, Chanez C, Jinek M
Nature (2021) 599 pp. 497-502 [ Pubmed: 34759315 DOI: 10.1038/s41586-021-04030-z ]
Image sets:
TniQ-capped Tns-ATP-dsDNA complex
Resolution: 3.44 Å
EM Method: Single-particle
Fitted PDBs: 8bd4
Q-score: 0.26
Schmitz M, Querques I, Oberli S, Chanez C, Jinek M
Cell (2022) 185 pp. 4999- [ DOI: doi:10.1016/j.cell.2022.11.009 Pubmed: 36435179 ]
- Zinc ion (65 Da, Ligand)
- Tnsc (31 kDa, Protein from Scytonema hofmannii)
- Complex of tnsc and tniq transposon proteins bound to atp and dsdna (Complex from Scytonema hofmannii)
- Dna (5'-d(p*gp*ap*tp*cp*gp*ap*tp*cp*gp*ap*tp*cp*gp*ap*tp*c)-3') (4 kDa, DNA from synthetic construct)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Tniq (homology model) (19 kDa, Protein from Scytonema hofmannii)
- Magnesium ion (24 Da, Ligand)
Cas12k-sgRNA-dsDNA-S15-TniQ-TnsC transposon recruitment complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8bd5
Q-score: 0.39
Schmitz M, Querques I, Oberli S, Chanez C, Jinek M
Cell (2022) 185 pp. 4999- [ DOI: doi:10.1016/j.cell.2022.11.009 Pubmed: 36435179 ]
- Shcas12k (79 kDa, Protein from Scytonema hofmannii)
- Zinc ion (65 Da, Ligand)
- Sgrna (82 kDa, RNA from Scytonema hofmannii)
- Dna non-target strand (15 kDa, DNA from synthetic construct)
- Tnsc (31 kDa, Protein from Scytonema hofmannii)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- Dna target strand (14 kDa, DNA from synthetic construct)
- Tniq (homology model) (19 kDa, Protein from Scytonema hofmannii)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Target dna bound cas12k-sgrna-s15-tniq-tnsc transposon recruitment complex (Complex from Scytonema hofmannii)
Cas12k-sgRNA-dsDNA-TnsC non-productive complex
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8bd6
Q-score: 0.149
Schmitz M, Querques I, Oberli S, Chanez C, Jinek M
Cell (2022) 185 pp. 4999- [ DOI: doi:10.1016/j.cell.2022.11.009 Pubmed: 36435179 ]
- Dna (12 kDa, DNA from synthetic construct)
- Dna non-target strand (15 kDa, DNA from synthetic construct)
- Sgrna (82 kDa, RNA from Scytonema hofmannii)
- Tnsc (31 kDa, Protein from Scytonema hofmannii)
- Target dna bound cas12k-sgrna-tnsc complex in non-productive state (Complex from Scytonema hofmannii)
- Dna target strand (14 kDa, DNA from synthetic construct)
- Cas12k (79 kDa, Protein from Scytonema hofmannii)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of ShCas12k in complex with a sgRNA and a dsDNA target
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 7pla
Q-score: 0.414
Querques I, Schmitz M, Oberli S, Chanez C, Jinek M
Nature (2021) 599 pp. 497-502 [ Pubmed: 34759315 DOI: doi:10.1038/s41586-021-04030-z ]
- Ternary complex of shcas12k with sgrna and target dsdna (187 kDa, Complex from Scytonema hofmannii)
- Sgrna (82 kDa, RNA from Scytonema hofmannii)
- Dna target strand (15 kDa, DNA from synthetic construct)
- Shcas12k (73 kDa, Protein from Scytonema hofmannii)
- Dna non-target strand (15 kDa, DNA from synthetic construct)
Scytonema hofmannii TnsC bound to AMPPNP and DNA
Resolution: 3.57 Å
EM Method: Helical reconstruction
Fitted PDBs: 7plh
Q-score: 0.415
Querques I, Schmitz M, Oberli S, Chanez C, Jinek M
Nature (2021) 599 pp. 497-502 [ Pubmed: 34759315 DOI: doi:10.1038/s41586-021-04030-z ]
- Dna (5'-d(p*ap*tp*ap*tp*ap*tp*ap*tp*ap*tp*ap*tp*ap*tp*ap*tp*ap*tp*ap*tp*ap*t)-3') (6 kDa, DNA from synthetic construct)
- Complex of shtnsc with amppnp and dsdna (Complex)
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Shtnsc (31 kDa, Protein from Scytonema hofmannii)