Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8wy4
Q-score: 0.505
Li J, Cheng R, Wang Z, Yuan W, Xiao J, Zhao X, Du X, Xia S, Wang L, Zhu B, Wang L
Nature (2024) 629 pp. 467-473 [ Pubmed: 38471529 DOI: doi:10.1038/s41586-024-07270-x ]
- Gaja tetramer with atp (Complex from Bacillus cereus VD045)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Endonuclease gaja (66 kDa, Protein from Bacillus cereus VD045)
Structure of Gabija GajA in complex with DNA
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 8wy5
Q-score: 0.414
Li J, Cheng R, Wang Z, Yuan W, Xiao J, Zhao X, Du X, Xia S, Wang L, Zhu B, Wang L
Nature (2024) 629 pp. 467-473 [ Pubmed: 38471529 DOI: doi:10.1038/s41586-024-07270-x ]
- Dna (5'-d(p*tp*tp*ap*ap*tp*ap*ap*cp*cp*cp*gp*gp*tp*tp*ap*tp*tp*tp*t)-3') (5 kDa, DNA from synthetic construct)
- Endonuclease gaja (67 kDa, Protein from Bacillus cereus VD045)
- Dna (5'-d(p*ap*ap*ap*ap*tp*ap*ap*cp*cp*gp*gp*gp*tp*tp*ap*tp*tp*ap*a)-3') (5 kDa, DNA from synthetic construct)
- Binary complex of gaja with dsdna (Complex from Bacillus cereus VD045)
- Calcium ion (40 Da, Ligand)
Structure of DNA-bound GajA dimer (focused refinement)
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 8x51
Q-score: 0.494
Li J, Cheng R, Wang Z, Yuan W, Xiao J, Zhao X, Du X, Xia S, Wang L, Zhu B, Wang L
Nature (2024) 629 pp. 467-473 [ Pubmed: 38471529 DOI: doi:10.1038/s41586-024-07270-x ]
- Endonuclease gaja (67 kDa, Protein from Bacillus cereus VD045)
- Binary complex of gaja with dsdna (Complex from Bacillus cereus VD045)
- Dna (5'-d(*tp*tp*tp*ap*ap*tp*ap*ap*cp*cp*cp*gp*gp*tp*tp*ap*tp*tp*tp*tp*t)-3') (6 kDa, DNA from synthetic construct)
- Dna (5'-d(*ap*ap*ap*ap*ap*tp*ap*ap*cp*cp*gp*gp*gp*tp*tp*ap*tp*tp*ap*ap*a)-3') (6 kDa, DNA from synthetic construct)
- Calcium ion (40 Da, Ligand)
Structure of ATP/Mg2+ bound Gabija GajA
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 8x5i
Q-score: 0.515
Li J, Cheng R, Wang Z, Yuan W, Xiao J, Zhao X, Du X, Xia S, Wang L, Zhu B, Wang L
Nature (2024) 629 pp. 467-473 [ Pubmed: 38471529 DOI: doi:10.1038/s41586-024-07270-x ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Binary complex of gabija with atp (Complex from Bacillus cereus VD045)
- Magnesium ion (24 Da, Ligand)
- Endonuclease gaja (67 kDa, Protein from Bacillus cereus VD045)
Structure of ATP/Mg2+ bound Gabija GajA-GajB 4:4 complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8x5n
Q-score: 0.487
Li J, Cheng R, Wang Z, Yuan W, Xiao J, Zhao X, Du X, Xia S, Wang L, Zhu B, Wang L
Nature (2024) 629 pp. 467-473 [ Pubmed: 38471529 DOI: doi:10.1038/s41586-024-07270-x ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Endonuclease gaja (67 kDa, Protein from Bacillus cereus VD045)
- Gaja tetramer with four gajb(atp binding state) (Complex from Bacillus cereus VD045)
- Gabija protein gajb (57 kDa, Protein from Bacillus cereus VD045)
Tetrahymena telomerase T3D2 structure at 3.3 Angstrom
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7lma
Q-score: 0.473
He Y, Wang Y, Liu B, Helmling C, Susac L, Cheng R, Zhou ZH, Feigon J
Nature (2021) 593 pp. 454-459 [ Pubmed: 33981033 DOI: doi:10.1038/s41586-021-03529-9 ]
- Telomerase la-related protein p65 (64 kDa, Protein from Tetrahymena thermophila)
- Telomere dna (9 kDa, DNA from Tetrahymena thermophila)
- Telomerase holoenzyme teb2 subunit (30 kDa, Protein from Tetrahymena thermophila)
- Zinc ion (65 Da, Ligand)
- Telomerase reverse transcriptase (133 kDa, Protein from Tetrahymena thermophila)
- Telomerase holoenzyme teb1 subunit (82 kDa, Protein from Tetrahymena thermophila)
- Telomerase holoenzyme teb3 subunit (14 kDa, Protein from Tetrahymena thermophila)
- Telomerase associated protein p50 (50 kDa, Protein from Tetrahymena thermophila)
- Telomerase rna (50 kDa, RNA from Tetrahymena thermophila)
- Tetrahymena telomerase t3d2 structure at 3.3 angstrom (Complex from Tetrahymena thermophila)
Tetrahymena telomerase T5D5 structure at 3.8 Angstrom
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7lmb
Q-score: 0.403
He Y, Wang Y, Liu B, Helmling C, Susac L, Cheng R, Zhou ZH, Feigon J
Nature (2021) 593 pp. 454-459 [ Pubmed: 33981033 DOI: doi:10.1038/s41586-021-03529-9 ]
- Telomerase la-related protein p65 (64 kDa, Protein from Tetrahymena thermophila)
- Telomerase holoenzyme teb2 subunit (30 kDa, Protein from Tetrahymena thermophila)
- Zinc ion (65 Da, Ligand)
- Telomerase reverse transcriptase (133 kDa, Protein from Tetrahymena thermophila)
- Telomerase holoenzyme teb1 subunit (82 kDa, Protein from Tetrahymena thermophila)
- Telomerase holoenzyme teb3 subunit (14 kDa, Protein from Tetrahymena thermophila)
- Tetrahymena telomerase t5d5 structure (Complex from Tetrahymena thermophila)
- Telomerase associated protein p50 (50 kDa, Protein from Tetrahymena thermophila)
- Telomerase rna (50 kDa, RNA from Tetrahymena thermophila)
- Telomere dna (6 kDa, DNA from Tetrahymena thermophila)