Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- Apo tric/cct (1 MDa, Complex from Homo sapiens)
- Cct subunit 7 (Macromolecule from Homo sapiens)
- Cct subunit 1 (Macromolecule from Homo sapiens)
- Cct subunit 8 (Macromolecule from Homo sapiens)
- Cct subunit 6 (Macromolecule from Homo sapiens)
- Cct subunit 2 (Macromolecule from Homo sapiens)
- Cct subunit 5 (Macromolecule from Homo sapiens)
- Cct subunit 4 (Macromolecule from Homo sapiens)
- Cct subunit 3 (Macromolecule from Homo sapiens)
The beta-tubulin folding intermediate I
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7trg
Q-score: 0.549
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- Water (18 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- Closed form human tric in complex with beta-tubulin under atp/alf3condition. (1 MDa, Complex from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
The beta-tubulin folding intermediate II
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7ttn
Q-score: 0.526
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- Closed form human tric in complex with beta-tubulin under atp/alfx condition. (1 MDa, Complex from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
The beta-tubulin folding intermediate III
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7ttt
Q-score: 0.561
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- Water (18 Da, Ligand)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- Closed form human tric in complex with beta-tubulin under atp/alfx condition. (1 MDa, Complex from Homo sapiens)
The beta-tubulin folding intermediate IV
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7tub
Q-score: 0.5
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- Water (18 Da, Ligand)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- Closed form human tric in complex with beta-tubulin under atp/alfx condition. (1 MDa, Complex from Homo sapiens)
Prefoldin-tubulin-TRiC complex
Resolution: 3.85 Å
EM Method: Single-particle
Fitted PDBs: 7wu7
Q-score: 0.302
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Prefoldin subunit 5 (16 kDa, Protein from Homo sapiens)
- Prefoldin subunit 4 (16 kDa, Protein from Homo sapiens)
- Prefoldin subunit 3 (22 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Prefoldin subunit 1 (14 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Prefoldin subunit 6 (14 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- Prefoldin subunit 2 (16 kDa, Protein from Homo sapiens)
- Ternary complex of tric/cct, beta-tubulin, prefoldin complex (1 MDa, Complex from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) hybrid module (M3/1) in complex with antibody fragment 1B2
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7m7e
Q-score: 0.449
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- Complex between debs m3/1te and antibody fragment 1b2 (430 kDa, Complex from Saccharopolyspora erythraea)
- 6-deoxyerythronolide-b synthase erya2, modules 3 and 4,eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (187 kDa, Protein from Saccharopolyspora erythraea)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 1
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7m7f
Q-score: 0.403
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- N~3~-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-n-(2-sulfanylethyl)-beta-alaninamide (358 Da, Ligand)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 2
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7m7g
Q-score: 0.181
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 1'
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7m7h
Q-score: 0.19
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)