cryo-EM structure of MBP-KIX-apoferritin
Resolution: 2.57 Å
EM Method: Single-particle
Fitted PDBs: 7tb3
Q-score: 0.382
Zhang K, Horikoshi N, Li S, Powers AS, Hameedi MA, Pintilie GD, Chae HD, Khan YA, Suomivuori CM, Dror RO, Sakamoto KM, Chiu W, Wakatsuki S
ACS Cent Sci (2022) 8 pp. 214-222 [ Pubmed: 35233453 DOI: doi:10.1021/acscentsci.1c01090 ]
- Isoform 2 of creb-binding protein,ferritin heavy chain, n-terminally processed (30 kDa, Protein from Homo sapiens)
- Mbp-kix-apoferritin (1 MDa, Complex from Homo sapiens)
cryo-EM structure of MBP-KIX-apoferritin complex with peptide 7
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 7tbh
Q-score: 0.361
Zhang K, Horikoshi N, Li S, Powers AS, Hameedi MA, Pintilie GD, Chae HD, Khan YA, Suomivuori CM, Dror RO, Sakamoto KM, Chiu W, Wakatsuki S
ACS Cent Sci (2022) 8 pp. 214-222 [ Pubmed: 35233453 DOI: doi:10.1021/acscentsci.1c01090 ]
- Isoform 2 of creb-binding protein,ferritin heavy chain, n-terminally processed (30 kDa, Protein from Homo sapiens)
- Leu-ser-arg-arg-pro-sep-tyr-arg-lys-ile-leu-asn-asp-leu-ser-ser-asp-ala-pro (2 kDa, Protein from Homo sapiens)
- Mbp-kix-apoferritin complex with peptide 7 (1 MDa, Complex from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) hybrid module (M3/1) in complex with antibody fragment 1B2
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7m7e
Q-score: 0.449
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- Complex between debs m3/1te and antibody fragment 1b2 (430 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
- 6-deoxyerythronolide-b synthase erya2, modules 3 and 4,eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (187 kDa, Protein from Saccharopolyspora erythraea)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 1
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7m7f
Q-score: 0.403
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- N~3~-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-n-(2-sulfanylethyl)-beta-alaninamide (358 Da, Ligand)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 2
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7m7g
Q-score: 0.181
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 1'
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7m7h
Q-score: 0.19
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2 (TE-free)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7m7i
Q-score: 0.304
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- N~3~-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-n-(2-sulfanylethyl)-beta-alaninamide (358 Da, Ligand)
- 1b2 (light chain) (26 kDa, Protein from Saccharopolyspora erythraea)
- 1b2 (heavy chain) (25 kDa, Protein from Homo sapiens)
- Eryai (167 kDa, Protein from Saccharopolyspora erythraea)
- Complex between debs (3)m1(2) and antibody fragment 1b2 (440 kDa, Complex from Saccharopolyspora erythraea)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7m7j
Q-score: 0.166
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ Pubmed: 34735239 DOI: doi:10.1126/science.abi8358 ]
- Eryai (167 kDa, Protein from Saccharopolyspora erythraea)
- Complex between debs (3)m1(2) and antibody fragment 1b2 (440 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
Resolution: 1.27 Å
EM Method: Single-particle
Fitted PDBs: 7rrp
Q-score: 0.908
Zhang K, Pintilie GD, Li S, Schmid MF, Chiu W
Cell Res (2020) 30 pp. 1136-1139 [ Pubmed: 33139928 DOI: doi:10.1038/s41422-020-00432-2 ]
- Zinc ion (65 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Ferritin heavy chain (20 kDa, Protein from Homo sapiens)
- Apoferritin (480 kDa, Complex from Homo sapiens)
- Water (18 Da, Ligand)
Resolution: 1.34 Å
EM Method: Single-particle
Fitted PDBs: 7k3v
Q-score: 0.899
Zhang K, Pintilie GD, Li S, Schmid MF, Chiu W
Cell Res (2020) 30 pp. 1136-1139 [ Pubmed: 33139928 DOI: doi:10.1038/s41422-020-00432-2 ]
- Zinc ion (65 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Ferritin heavy chain (20 kDa, Protein from Homo sapiens)
- Apoferritin (480 kDa, Complex from Homo sapiens)
- Water (18 Da, Ligand)