The beta-tubulin folding intermediate I
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7trg
Q-score: 0.549
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- Closed form human tric in complex with beta-tubulin under atp/alf3condition. (1 MDa, Complex from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
The beta-tubulin folding intermediate II
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7ttn
Q-score: 0.526
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Aluminum fluoride (83 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Closed form human tric in complex with beta-tubulin under atp/alfx condition. (1 MDa, Complex from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
The beta-tubulin folding intermediate III
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7ttt
Q-score: 0.561
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- Closed form human tric in complex with beta-tubulin under atp/alfx condition. (1 MDa, Complex from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
The beta-tubulin folding intermediate IV
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7tub
Q-score: 0.5
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- Closed form human tric in complex with beta-tubulin under atp/alfx condition. (1 MDa, Complex from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
Prefoldin-tubulin-TRiC complex
Resolution: 3.85 Å
EM Method: Single-particle
Fitted PDBs: 7wu7
Q-score: 0.302
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Prefoldin subunit 6 (14 kDa, Protein from Homo sapiens)
- Prefoldin subunit 2 (16 kDa, Protein from Homo sapiens)
- Prefoldin subunit 5 (16 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Prefoldin subunit 4 (16 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- Prefoldin subunit 1 (14 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- Prefoldin subunit 3 (22 kDa, Protein from Homo sapiens)
- Ternary complex of tric/cct, beta-tubulin, prefoldin complex (1 MDa, Complex from Homo sapiens)
Human TRiC/CCT complex with reovirus outer capsid protein sigma-3
Resolution: 6.2 Å
EM Method: Single-particle
Fitted PDBs: 7lup
Q-score: 0.217
Knowlton JJ, Gestaut D, Ma B, Taylor G, Seven AB, Leitner A, Wilson GJ, Shanker S, Yates NA, Prasad BVV, Aebersold R, Chiu W, Frydman J, Dermody TS
PNAS (2021) 118 [ Pubmed: 33836586 DOI: doi:10.1073/pnas.2018127118 ]
- Human tric/cct complex with reovirus outer capsid protein sigma-3 (41 MDa, Complex)
- Zinc ion (65 Da, Ligand)
- Reovirus outer capsid protein sigma-3 (Complex from Mammalian orthoreovirus 3 Dearing)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Outer capsid protein sigma-3 (41 kDa, Protein from Reovirus type 3 (strain Dearing))
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- Human tric/cct complex (Complex from Homo sapiens)
- Water (18 Da, Ligand)