The beta-tubulin folding intermediate I
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7trg
Q-score: 0.549
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- Closed form human tric in complex with beta-tubulin under atp/alf3condition. (1 MDa, Complex from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
The beta-tubulin folding intermediate II
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7ttn
Q-score: 0.526
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Aluminum fluoride (83 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Closed form human tric in complex with beta-tubulin under atp/alfx condition. (1 MDa, Complex from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
The beta-tubulin folding intermediate III
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7ttt
Q-score: 0.561
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- Closed form human tric in complex with beta-tubulin under atp/alfx condition. (1 MDa, Complex from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
The beta-tubulin folding intermediate IV
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7tub
Q-score: 0.5
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- Closed form human tric in complex with beta-tubulin under atp/alfx condition. (1 MDa, Complex from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
Prefoldin-tubulin-TRiC complex
Resolution: 3.85 Å
EM Method: Single-particle
Fitted PDBs: 7wu7
Q-score: 0.302
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Prefoldin subunit 6 (14 kDa, Protein from Homo sapiens)
- Prefoldin subunit 2 (16 kDa, Protein from Homo sapiens)
- Prefoldin subunit 5 (16 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Prefoldin subunit 4 (16 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- Prefoldin subunit 1 (14 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- Prefoldin subunit 3 (22 kDa, Protein from Homo sapiens)
- Ternary complex of tric/cct, beta-tubulin, prefoldin complex (1 MDa, Complex from Homo sapiens)
Resolution: 1.27 Å
EM Method: Single-particle
Fitted PDBs: 7rrp
Q-score: 0.908
Zhang K, Pintilie GD, Li S, Schmid MF, Chiu W
Cell Res (2020) 30 pp. 1136-1139 [ Pubmed: 33139928 DOI: doi:10.1038/s41422-020-00432-2 ]
- Zinc ion (65 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Ferritin heavy chain (20 kDa, Protein from Homo sapiens)
- Apoferritin (480 kDa, Complex from Homo sapiens)
- Water (18 Da, Ligand)
Resolution: 1.34 Å
EM Method: Single-particle
Fitted PDBs: 7k3v
Q-score: 0.899
Zhang K, Pintilie GD, Li S, Schmid MF, Chiu W
Cell Res (2020) 30 pp. 1136-1139 [ Pubmed: 33139928 DOI: doi:10.1038/s41422-020-00432-2 ]
- Zinc ion (65 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Ferritin heavy chain (20 kDa, Protein from Homo sapiens)
- Apoferritin (480 kDa, Complex from Homo sapiens)
- Water (18 Da, Ligand)
Resolution: 1.36 Å
EM Method: Single-particle
Fitted PDBs: 7k3w
Q-score: 0.9
Zhang K, Pintilie GD, Li S, Schmid MF, Chiu W
Cell Res (2020) 30 pp. 1136-1139 [ Pubmed: 33139928 DOI: doi:10.1038/s41422-020-00432-2 ]
- Zinc ion (65 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Ferritin heavy chain (20 kDa, Protein from Homo sapiens)
- Apoferritin (480 kDa, Complex from Homo sapiens)
- Water (18 Da, Ligand)
Human Drosha and DGCR8 in complex with Primary MicroRNA (MP/RNA complex) - Active state
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6v5b
Q-score: 0.337
Partin AC, Zhang K, Jeong BC, Herrell E, Li S, Chiu W, Nam Y
Mol Cell (2020) 78 pp. 411- [ DOI: doi:10.1016/j.molcel.2020.02.016 Pubmed: 32220646 ]
- Zinc ion (65 Da, Ligand)
- Pri-mir-16-2 (78-mer) (33 kDa, RNA from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Human drosha and dgcr8 in complex with primary microrna (mp/rna complex) - active state (40 kDa, Complex from Homo sapiens)
- Ribonuclease 3 (118 kDa, Protein from Homo sapiens)
- Microprocessor complex subunit dgcr8 (60 kDa, Protein from Homo sapiens)
Small conformation of ssRNA-bound CRISPR_Csm complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6nud
Q-score: 0.254
Guo M, Zhang K, Zhu Y, Pintilie GD, Guan X, Li S, Schmid MF, Ma Z, Chiu W, Huang Z
Cell Res (2019) 29 pp. 305-312 [ Pubmed: 30814678 DOI: doi:10.1038/s41422-019-0151-x ]
- Crispr type iii-associated ramp protein csm4 (33 kDa, Protein from Streptococcus thermophilus)
- Crrna (22 kDa, RNA from Streptococcus thermophilus)
- Target ssrna (15 kDa, RNA from Streptococcus thermophilus)
- Crispr system cms protein csm2 (14 kDa, Protein from Streptococcus thermophilus)
- Crispr type iii-associated ramp protein csm3 (24 kDa, Protein from Streptococcus thermophilus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Small conformation of ssrna-bound crispr_csm complex (Complex from Streptococcus thermophilus)
- Crispr system single-strand-specific deoxyribonuclease cas10/csm1 (subtype iii-a) (86 kDa, Protein from Streptococcus thermophilus)