The symmetry-released subpellicular microtubule map from detergent-extracted Toxoplasma cells
Resolution: 8.4 Å
EM Method: Subtomogram averaging
Fitted PDBs: 7tnq
Q-score: 0.076
Sun SY, Segev-Zarko LA, Chen M, Pintilie GD, Schmid MF, Ludtke SJ, Boothroyd JC, Chiu W
PNAS (2022) 119 [ Pubmed: 35121661 DOI: doi:10.1073/pnas.2111661119 ]
- Microtubule associated protein spm1 (38 kDa, Protein from Toxoplasma gondii)
- Tubulin beta chain (50 kDa, Protein from Toxoplasma gondii)
- Tubulin alpha chain (50 kDa, Protein from Toxoplasma gondii)
- Microtubule complex of tubulin and intra-luminal proteins (Cellular component from Toxoplasma gondii)
- Pdi family protein (24 kDa, Protein from Toxoplasma gondii)
- Pdi family protein (21 kDa, Protein from Toxoplasma gondii)
Subpellicular microtubule from detergent-extract Toxoplasma gondii cells
Resolution: 6.7 Å
EM Method: Subtomogram averaging
Fitted PDBs: 7tns
Q-score: 0.118
Sun SY, Segev-Zarko LA, Chen M, Pintilie GD, Schmid MF, Ludtke SJ, Boothroyd JC, Chiu W
PNAS (2022) 119 [ Pubmed: 35121661 DOI: doi:10.1073/pnas.2111661119 ]
- Microtubule associated protein spm1 (38 kDa, Protein from Toxoplasma gondii)
- Microtubule complex of alpha-tubulin and beta-tubulin with intra-lumenal proteins (Cellular component from Toxoplasma gondii)
- Tubulin beta chain (50 kDa, Protein from Toxoplasma gondii)
- Tubulin alpha chain (50 kDa, Protein from Toxoplasma gondii)
- Pdi family protein (24 kDa, Protein from Toxoplasma gondii)
- Pdi family protein (21 kDa, Protein from Toxoplasma gondii)
The tubulin-based conoid from detergent-extract Toxoplasma gondii cells
Resolution: 9.3 Å
EM Method: Subtomogram averaging
Fitted PDBs: 7tnt
Q-score: 0.053
Sun SY, Segev-Zarko LA, Chen M, Pintilie GD, Schmid MF, Ludtke SJ, Boothroyd JC, Chiu W
PNAS (2022) 119 [ Pubmed: 35121661 DOI: doi:10.1073/pnas.2111661119 ]
- Tubulin beta chain (47 kDa, Protein from Toxoplasma gondii)
- Tubulin alpha chain (48 kDa, Protein from Toxoplasma gondii)
- A conoid complex of tubulin with other protein components (Cellular component from Toxoplasma gondii)
Structure of full-length IP3R1 channel in the apo-state determined by single particle cryo-EM
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 3jav
Q-score: 0.219
Fan G, Baker ML, Wang Z, Baker MR, Sinyagovskiy PA, Chiu W, Ludtke SJ, Serysheva II
Nature (2015) 527 pp. 336-341 [ DOI: doi:10.1038/nature15249 Pubmed: 26458101 ]
- Inositol 1,4,5-trisphosphate receptor (300 kDa, Protein from Rattus norvegicus)
- Inositol 1,4,5-trisphosphate receptor, type 1 (1 MDa, Sample)
Yi P, Wang Z, Feng Q, Pintilie GD, Foulds CE, Lanz RB, Ludtke SJ, Schmid MF, Chiu W, O'Malley BW
Mol Cell (2015) 57 pp. 1047-1058 [ Pubmed: 25728767 DOI: doi:10.1016/j.molcel.2015.01.025 ]
- P300 (300 kDa, Sample)
- Histone acetyltransferase p300 (300 kDa, Protein from Homo sapiens)
Yi P, Wang Z, Feng Q, Pintilie GD, Foulds CE, Lanz RB, Ludtke SJ, Schmid MF, Chiu W, O'Malley BW
Mol Cell (2015) 57 pp. 1047-1058 [ Pubmed: 25728767 DOI: doi:10.1016/j.molcel.2015.01.025 ]
- Antibody 1 (Protein from unidentified)
- Histone acetyltransferase p300 (300 kDa, Protein from Homo sapiens)
- P300 bound to antibody 1 (400 kDa, Sample)
Yi P, Wang Z, Feng Q, Pintilie GD, Foulds CE, Lanz RB, Ludtke SJ, Schmid MF, Chiu W, O'Malley BW
Mol Cell (2015) 57 pp. 1047-1058 [ Pubmed: 25728767 DOI: doi:10.1016/j.molcel.2015.01.025 ]
- Antibody 2 (Protein from unidentified)
- P300 bound to antibody 2 (400 kDa, Sample)
- Histone acetyltransferase p300 (300 kDa, Protein from Homo sapiens)
cryo-EM structure of CCT5 complex with 1mM ATP/AlFx (C8 symmetry)
Sergeeva OA, Chen B, Haase-Pettingell C, Ludtke SJ, Chiu W, King JA
J Biol Chem (2013) 288 pp. 17734-17744 [ DOI: doi:10.1074/jbc.M112.443929 Pubmed: 23612981 ]
- Cct5 complex (1 MDa, Sample)
- Chaperonin containing tcp1, subunit 5 (epsilon) (1 MDa, Protein from Homo sapiens)
cryo-EM structure of CCT5 complex with 1mM ATP/AlFx (symmetry-free)
Sergeeva OA, Chen B, Haase-Pettingell C, Ludtke SJ, Chiu W, King JA
J Biol Chem (2013) 288 pp. 17734-17744 [ DOI: doi:10.1074/jbc.M112.443929 Pubmed: 23612981 ]
- Cct5 complex (1 MDa, Sample)
- Chaperonin containing tcp1, subunit 5 (epsilon) (1 MDa, Protein from Homo sapiens)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 3j32
Q-score: 0.203