Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 7py0
Q-score: 0.185
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ DOI: doi:10.1038/s41467-022-29148-0 Pubmed: 35318334 ]
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Nusg elongation complex in more-swiveled conformation (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Tdna (11 kDa, DNA from Escherichia coli)
- Ntdna (12 kDa, DNA from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7py6
Q-score: 0.348
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ DOI: doi:10.1038/s41467-022-29148-0 Pubmed: 35318334 ]
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Tdna (11 kDa, DNA from Escherichia coli)
- Ntdna (12 kDa, DNA from Escherichia coli)
- Nusa and nusg elongation complex in non-swiveled conformation (Complex from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7py7
Q-score: 0.329
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ DOI: doi:10.1038/s41467-022-29148-0 Pubmed: 35318334 ]
- Zinc ion (65 Da, Ligand)
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli MS 115-1)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Ntdna (12 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Tdna (11 kDa, DNA from Escherichia coli)
- Nusa and nusg elongation complex in more-swiveled conformation (Complex from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7pyj
Q-score: 0.276
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ DOI: doi:10.1038/s41467-022-29148-0 Pubmed: 35318334 ]
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Nusa elongation complex in non-swiveled conformation (Complex from Escherichia coli)
- Ntdna (12 kDa, DNA from Escherichia coli)
- Tdna (11 kDa, DNA from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7pyk
Q-score: 0.295
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ DOI: doi:10.1038/s41467-022-29148-0 Pubmed: 35318334 ]
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Tdna (11 kDa, DNA from Escherichia coli)
- Ntdna (12 kDa, DNA from Escherichia coli)
- Nusa elongation complex in more-swiveled conformation (Complex from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
RNA polymerase elongation complex in more-swiveled conformation
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7q0j
Q-score: 0.304
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ DOI: doi:10.1038/s41467-022-29148-0 Pubmed: 35318334 ]
- Rna polymerase elongation complex in more-swiveled conformation (Complex from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Tdna (11 kDa, DNA from Escherichia coli)
- Ntdna (12 kDa, DNA from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
RNA polymerase elongation complex in less-swiveled conformation
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7q0k
Q-score: 0.299
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ DOI: doi:10.1038/s41467-022-29148-0 Pubmed: 35318334 ]
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Rna polymerase elongation complex in less-swiveled conformation (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Tdna (11 kDa, DNA from Escherichia coli)
- Ntdna (12 kDa, DNA from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6flp
Q-score: 0.334
Guo X, Myasnikov AG, Chen J, Crucifix C, Papai G, Takacs M, Schultz P, Weixlbaumer A
Mol Cell (2018) 69 pp. 816-827.e4 [ DOI: doi:10.1016/j.molcel.2018.02.008 Pubmed: 29499136 ]
- Nucleic acids (Complex from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli (strain K12))
- Cryo-em structure of e. coli rna polymerase paused elongation complex without rna hairpin bound to nusa (Complex)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Dna (30-mer) (9 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Dna (39-mer) (11 kDa, DNA from synthetic construct)
- Rna (5'-r(p*gp*ap*up*gp*up*gp*up*gp*cp*u)-3') (3 kDa, RNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Rna polymerase and nusa (Complex from Escherichia coli K-12)