Complex of RecF and DNA from Thermus thermophilus.
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8a8j
Q-score: 0.556
Nirwal S, Czarnocki-Cieciura M, Chaudhary A, Zajko W, Skowronek K, Chamera S, Figiel M, Nowotny M
Nat Struct Mol Biol (2023) 30 pp. 650-660 [ DOI: doi:10.1038/s41594-023-00967-z Pubmed: 37081315 ]
- Complex of recf and dna from thermus thermophilus. (95 kDa, Complex from Thermus thermophilus HB8)
- Oligo2 (12 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna replication and repair protein recf (37 kDa, Protein from Thermus thermophilus HB8)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Oligo1 (7 kDa, DNA from synthetic construct)
Complex of RecF-RecR-DNA from Thermus thermophilus.
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 8a93
Q-score: 0.525
Nirwal S, Czarnocki-Cieciura M, Chaudhary A, Zajko W, Skowronek K, Chamera S, Figiel M, Nowotny M
Nat Struct Mol Biol (2023) 30 pp. 650-660 [ DOI: doi:10.1038/s41594-023-00967-z Pubmed: 37081315 ]
- Dna replication and repair protein recf (37 kDa, Protein from Thermus thermophilus HB8)
- Recombination protein recr (21 kDa, Protein from Thermus thermophilus HB8)
- Oligo2 (12 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Complex of recf-recr-dna from thermus thermophilus. (180 kDa, Complex from Thermus thermophilus HB8)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Oligo1 (7 kDa, DNA from synthetic construct)
Complex of RecO-RecR-DNA from Thermus thermophilus.
Resolution: 6.09 Å
EM Method: Single-particle
Fitted PDBs: 8ab0
Q-score: 0.233
Nirwal S, Czarnocki-Cieciura M, Chaudhary A, Zajko W, Skowronek K, Chamera S, Figiel M, Nowotny M
Nat Struct Mol Biol (2023) 30 pp. 650-660 [ DOI: doi:10.1038/s41594-023-00967-z Pubmed: 37081315 ]
- Zinc ion (65 Da, Ligand)
- Dna repair protein reco (24 kDa, Protein from Thermus thermophilus HB8)
- Recombination protein recr (21 kDa, Protein from Thermus thermophilus HB8)
- Complex of reco-recr-dna from thermus thermophilus. (130 kDa, Complex from Thermus thermophilus HB8)
- Oligo2 (12 kDa, DNA from synthetic construct)
- Oligo1 (7 kDa, DNA from synthetic construct)
Complex of RecF-RecO-RecR-DNA from Thermus thermophilus (low resolution reconstruction).
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 8bpr
Q-score: 0.359
Nirwal S, Czarnocki-Cieciura M, Chaudhary A, Zajko W, Skowronek K, Chamera S, Figiel M, Nowotny M
Nat Struct Mol Biol (2023) 30 pp. 650-660 [ DOI: doi:10.1038/s41594-023-00967-z Pubmed: 37081315 ]
- Complex of recf-reco-recr-dna from thermus thermophilus. (205 kDa, Complex from Thermus thermophilus HB8)
- Zinc ion (65 Da, Ligand)
- Dna repair protein reco (24 kDa, Protein from Thermus thermophilus HB8)
- Recombination protein recr (21 kDa, Protein from Thermus thermophilus HB8)
- Oligo2 (12 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna replication and repair protein recf (37 kDa, Protein from Thermus thermophilus HB8)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Oligo1 (7 kDa, DNA from synthetic construct)
The architecture of the S. pombe CCR4-NOT complex
Ukleja M, Cuellar J, Siwaszek J, Kasprzak JM, Czarnocki-Cieciura M, Bujnicki J, Dziembowski A, Valpuesta JM
Nat Commun (2016) 7 pp. 10433- [ DOI: doi:10.1038/ncomms10433 Pubmed: 26804377 ]
- S. pombe ccr4-not complex (700 MDa, Sample)
- Ccr4-not complex (700 MDa, Protein from Schizosaccharomyces pombe)
Electron Microscopy of THO complex
Pena A, Gewartowski K, Mroczek S, Cuellar J, Szykowska A, Prokop A, Czarnocki-Cieciura M, Piwowarski J, Tous C, Aguilera A, Carrascosa JL, Valpuesta JM, Dziembowski A
EMBO J (2012) 31 pp. 1605-1616 [ Pubmed: 22314234 DOI: doi:10.1038/emboj.2012.10 ]
- Tex1 (50 MDa, Protein from Saccharomyces cerevisiae)
- Mft1 (45 MDa, Protein from Saccharomyces cerevisiae)
- Hpr1 (90 MDa, Protein from Saccharomyces cerevisiae)
- Thp2 (30 MDa, Protein from Saccharomyces cerevisiae)
- Tho2 (180 MDa, Protein from Saccharomyces cerevisiae)
- Yeast five-subunit tho complex (395 MDa, Sample)