Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8odz
Q-score: 0.329
Bloch Y, Felix J, Merceron R, Provost M, Symakani RA, De Backer R, Lambert E, Mehdipour AR, Savvides SN
Nat Struct Mol Biol (2024) 31 pp. 591-597 [ Pubmed: 38287195 DOI: doi:10.1038/s41594-023-01190-6 ]
- Interleukin-12 receptor subunit beta-2,calmodulin-1 (85 kDa, Protein from Mus musculus)
- Interleukin-12 subunit beta (35 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
- Interleukin-12 receptor subunit beta-1,death-associated protein kinase 1 (63 kDa, Protein from Mus musculus)
- Interleukin-12 subunit alpha (25 kDa, Protein from Mus musculus)
- Murine il-12 in complex with mil-12rbeta1-dapk1 and mil-12rbeta2-calmodulin. (216 kDa, Complex from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 8oe0
Q-score: 0.208
Bloch Y, Felix J, Merceron R, Provost M, Symakani RA, De Backer R, Lambert E, Mehdipour AR, Savvides SN
Nat Struct Mol Biol (2024) 31 pp. 591-597 [ Pubmed: 38287195 DOI: doi:10.1038/s41594-023-01190-6 ]
- Interleukin-12 receptor subunit beta-2,calmodulin-1 (85 kDa, Protein from Mus musculus)
- Interleukin-12 subunit beta (35 kDa, Protein from Mus musculus)
- Interleukin-12 receptor subunit beta-1,death-associated protein kinase 1 (63 kDa, Protein from Mus musculus)
- Interleukin-12 subunit alpha (25 kDa, Protein from Mus musculus)
- Murine il-12 in complex with mil-12rbeta1-dapk1 and mil-12rbeta2-calmodulin. (216 kDa, Complex from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Cryo-EM structure of the murine IL-12 complete extracellular signaling complex (Class 1).
Bloch Y, Felix J, Merceron R, Provost M, Symakani RA, De Backer R, Lambert E, Mehdipour AR, Savvides SN
Nat Struct Mol Biol (2024) 31 pp. 591-597 [ Pubmed: 38287195 DOI: doi:10.1038/s41594-023-01190-6 ]
- Murine interleukin-12 subunit alpha (il-12a, p35), with a c-terminal caspase-3 cleavable his tag. (Protein from Mus musculus)
- Murine interleukin-12 subunit beta (il-12b, p40). (Protein from Mus musculus)
- Murine interleukin-12 receptor beta 2 (domains 1 to 6) with c-terminal strep-ii tag. (Protein from Mus musculus)
- Murine interleukin-12 receptor beta 1 (domains 1 to 5) with c-terminal strep-ii tag. (Protein from Mus musculus)
- Murine il-12 in complex with strep-ii tagged mil-12rbeta1 and mil-12rbeta2. (198 kDa, Complex from Mus musculus)
Cryo-EM structure of the murine IL-12 complete extracellular signaling complex (Class 2).
Bloch Y, Felix J, Merceron R, Provost M, Symakani RA, De Backer R, Lambert E, Mehdipour AR, Savvides SN
Nat Struct Mol Biol (2024) 31 pp. 591-597 [ Pubmed: 38287195 DOI: doi:10.1038/s41594-023-01190-6 ]
- Murine interleukin-12 subunit alpha (il-12a, p35), with a c-terminal caspase-3 cleavable his tag. (Protein from Mus musculus)
- Murine interleukin-12 subunit beta (il-12b, p40). (Protein from Mus musculus)
- Murine interleukin-12 receptor beta 2 (domains 1 to 6) with c-terminal strep-ii tag. (Protein from Mus musculus)
- Murine interleukin-12 receptor beta 1 (domains 1 to 5) with c-terminal strep-ii tag. (Protein from Mus musculus)
- Murine il-12 in complex with strep-ii tagged mil-12rbeta1 and mil-12rbeta2. (198 kDa, Complex from Mus musculus)
Cryo-EM structure of a pre-dimerized human IL-23 complete extracellular signaling complex.
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8oe4
Q-score: 0.294
Bloch Y, Felix J, Merceron R, Provost M, Symakani RA, De Backer R, Lambert E, Mehdipour AR, Savvides SN
Nat Struct Mol Biol (2024) 31 pp. 591-597 [ Pubmed: 38287195 DOI: doi:10.1038/s41594-023-01190-6 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Interleukin-23 subunit alpha (20 kDa, Protein from Homo sapiens)
- Interleukin-12 subunit beta (34 kDa, Protein from Homo sapiens)
- Interleukin-23 receptor,calmodulin-1 (54 kDa, Protein from Homo sapiens)
- Human il-23 in complex with hil-12rbeta1-dapk1 and hil-23r-calmodulin. (168 kDa, Complex from Mus musculus)
- Interleukin-12 receptor subunit beta-1,death-associated protein kinase 1 (60 kDa, Protein from Homo sapiens)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8pb1
Q-score: 0.424
Bloch Y, Felix J, Merceron R, Provost M, Symakani RA, De Backer R, Lambert E, Mehdipour AR, Savvides SN
Nat Struct Mol Biol (2024) 31 pp. 591-597 [ Pubmed: 38287195 DOI: doi:10.1038/s41594-023-01190-6 ]
- Interleukin-12 receptor subunit beta-2,calmodulin-1 (85 kDa, Protein from Mus musculus)
- Interleukin-12 subunit beta (35 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
- Interleukin-12 receptor subunit beta-1,death-associated protein kinase 1 (63 kDa, Protein from Mus musculus)
- Interleukin-12 subunit alpha (25 kDa, Protein from Mus musculus)
- Murine il-12 in complex with mil-12rbeta1-dapk1 and mil-12rbeta2-calmodulin. (216 kDa, Complex from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)