Cryo-EM structure of Arf1-decorated membrane tubules
Resolution: 3.1 Å
EM Method: Helical reconstruction
Fitted PDBs: 8s5c
Q-score: 0.508
Haupt C, Semchonok DA, Stubbs MT, Bacia K, Desfosses A
To Be Published
- Water (18 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Adp-ribosylation factor 1 (20 kDa, Protein from Saccharomyces cerevisiae)
- Arf1 coated membrane tubules (Complex from Saccharomyces cerevisiae)
- 5'-guanosine-diphosphate-monothiophosphate (539 Da, Ligand)
Cryo-EM structure of Arf1-decorated membrane tubules
Resolution: 3.79 Å
EM Method: Helical reconstruction
Fitted PDBs: 8s5d
Q-score: 0.302
Haupt C, Semchonok DA, Stubbs MT, Bacia K, Desfosses A
To Be Published
- Water (18 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Adp-ribosylation factor 1 (20 kDa, Protein from Saccharomyces cerevisiae)
- Arf1 coated membrane tubules (Complex from Saccharomyces cerevisiae)
- 5'-guanosine-diphosphate-monothiophosphate (539 Da, Ligand)
Cryo-EM structure of Arf1-decorated membrane tubules
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8s5e
Q-score: 0.569
Haupt C, Semchonok DA, Stubbs MT, Bacia K, Desfosses A
Cell Structure (2024)
- Water (18 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Adp-ribosylation factor 1 (20 kDa, Protein from Saccharomyces cerevisiae)
- Heptameric complex of arf1 with gsp and mg (Complex from Saccharomyces cerevisiae)
- 5'-guanosine-diphosphate-monothiophosphate (539 Da, Ligand)
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ Pubmed: 39048819 DOI: doi:10.1038/s41586-024-07720-6 ]
Native eisosome lattice bound to plasma membrane microdomain
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8qb7
Q-score: 0.323
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ Pubmed: 39048819 DOI: doi:10.1038/s41586-024-07720-6 ]
Resolution: 3.35 Å
EM Method: Helical reconstruction
Fitted PDBs: 8qb9
Q-score: 0.478
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ Pubmed: 39048819 DOI: doi:10.1038/s41586-024-07720-6 ]
Resolution: 3.86 Å
EM Method: Helical reconstruction
Fitted PDBs: 8qbb
Q-score: 0.404
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ Pubmed: 39048819 DOI: doi:10.1038/s41586-024-07720-6 ]
- Helical assembly of recombinant pil1 protein tubulating +pip2/-sterol lipid mixture (dopc,dope,dops,pi(4,5)p2 50:20:20:10) (Complex from Saccharomyces cerevisiae)
- D-myo-inositol-1,4,5-triphosphate (420 Da, Ligand)
- Sphingolipid long chain base-responsive protein pil1 (38 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 3.61 Å
EM Method: Helical reconstruction
Fitted PDBs: 8qbd
Q-score: 0.428
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ Pubmed: 39048819 DOI: doi:10.1038/s41586-024-07720-6 ]
- D-myo-inositol-1,4,5-triphosphate (420 Da, Ligand)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- Helical assembly of recombinant pil1 protein tubulating +pip2/+sterol lipid mixture (dopc, dope, dops, cholesterol, pi(4,5)p2 35:20:20:15:10) (Complex from Saccharomyces cerevisiae)
- Sphingolipid long chain base-responsive protein pil1 (38 kDa, Protein from Saccharomyces cerevisiae)
Compact state - Native eisosome lattice bound to plasma membrane microdomain
Resolution: 3.67 Å
EM Method: Single-particle
Fitted PDBs: 8qbe
Q-score: 0.228
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ Pubmed: 39048819 DOI: doi:10.1038/s41586-024-07720-6 ]
Stretched state - Native eisosome lattice bound to plasma membrane microdomain
Resolution: 3.64 Å
EM Method: Single-particle
Fitted PDBs: 8qbg
Q-score: 0.217
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ Pubmed: 39048819 DOI: doi:10.1038/s41586-024-07720-6 ]