Map of GroEL:GroES-(ADP) complex plunge frozen 200 ms after reaction initiation with ATP
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023) 20 pp. 1400-1408 [ Pubmed: 37592181 DOI: doi:10.1038/s41592-023-01967-z ]
- Groel (Protein from Escherichia coli)
- Hetero 14mer assembled from 2 heptameric rings (950 kDa, Complex from Escherichia coli)
- Groes (Protein from Escherichia coli)
Movies of GroEL-ES-nucleotide complex plunged 13, 50, 200 ms and 20 s after mixing with ATP
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods () [ DOI: 10.1038/s41592-023-01967-z ]
Image sets:
- GroELES with 20s of mixing time
- GroEL 13ms timepoint
- GroELES 200ms timepoint
- GroELES at 50ms timepoint
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8bk7
Q-score: 0.525
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023) 20 pp. 1400-1408 [ Pubmed: 37592181 DOI: doi:10.1038/s41592-023-01967-z ]
- Beta-galactosidase from e.coli (Complex from Escherichia coli)
- Beta-galactosidase (117 kDa, Protein from Escherichia coli K-12)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8bk8
Q-score: 0.609
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023) 20 pp. 1400-1408 [ Pubmed: 37592181 DOI: doi:10.1038/s41592-023-01967-z ]
- Beta-galactosidase (117 kDa, Protein from Escherichia coli)
- Beta-galactosidase (Complex from Escherichia coli)
Resolution: 2.1 Å
EM Method: Single-particle
Fitted PDBs: 8bk9
Q-score: 0.775
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023) 20 pp. 1400-1408 [ Pubmed: 37592181 DOI: doi:10.1038/s41592-023-01967-z ]
- Water (18 Da, Ligand)
- Mouse heavy chain apoferritin from e.coli (Complex from Mus musculus)
- Ferritin heavy chain (21 kDa, Protein from Mus musculus)
- Fe (iii) ion (55 Da, Ligand)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8bka
Q-score: 0.669
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023) 20 pp. 1400-1408 [ Pubmed: 37592181 DOI: doi:10.1038/s41592-023-01967-z ]
- Water (18 Da, Ligand)
- Mouse heavy chain apoferritin from e.coli (Complex from Mus musculus)
- Ferritin heavy chain (21 kDa, Protein from Mus musculus)
- Fe (iii) ion (55 Da, Ligand)
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8bkb
Q-score: 0.78
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023) 20 pp. 1400-1408 [ Pubmed: 37592181 DOI: doi:10.1038/s41592-023-01967-z ]
- Water (18 Da, Ligand)
- Mouse heavy chain apoferritin from e.coli (Complex from Mus musculus)
- Ferritin heavy chain (21 kDa, Protein from Mus musculus)
- Fe (iii) ion (55 Da, Ligand)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8bkg
Q-score: 0.536
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023) 20 pp. 1400-1408 [ Pubmed: 37592181 DOI: doi:10.1038/s41592-023-01967-z ]
- Beta-galactosidase (Complex from Escherichia coli)
- Beta-galactosidase (117 kDa, Protein from Escherichia coli K-12)
GroEL:GroES-ATP complex under continuous turnover condirions
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8bkz
Q-score: 0.632
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023) 20 pp. 1400-1408 [ Pubmed: 37592181 DOI: doi:10.1038/s41592-023-01967-z ]
- Co-chaperonin groes (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Water (18 Da, Ligand)
- Chaperonin groel (57 kDa, Protein from Escherichia coli)
- Potassium ion (39 Da, Ligand)
- Hetero 14mer assembled from 2 heptameric rings (950 kDa, Complex from Escherichia coli)
Structure of GroEL-ATP complex plunge frozen 200 ms after reaction initiation
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8bl2
Q-score: 0.465
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023) 20 pp. 1400-1408 [ Pubmed: 37592181 DOI: doi:10.1038/s41592-023-01967-z ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Water (18 Da, Ligand)
- Chaperonin groel (57 kDa, Protein from Escherichia coli)
- Potassium ion (39 Da, Ligand)
- Groel in complex with atp (800 kDa, Complex from Escherichia coli)