Cryo-EM structure of murine Dispatched 'R' conformation
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7rph
Q-score: 0.57
Wang Q, Asarnow DE, Ding K, Mann RK, Hatakeyama J, Zhang Y, Ma Y, Cheng Y, Beachy PA
Nature (2021) 599 pp. 320-324 [ Pubmed: 34707294 DOI: doi:10.1038/s41586-021-03996-0 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Dispatched protein 'r' conformation (151 kDa, Complex from Mus musculus)
- Sodium ion (22 Da, Ligand)
- Protein dispatched homolog 1 (152 kDa, Protein from Mus musculus)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Water (18 Da, Ligand)
Cryo-EM structure of murine Dispatched 'T' conformation
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7rpi
Q-score: 0.554
Wang Q, Asarnow DE, Ding K, Mann RK, Hatakeyama J, Zhang Y, Ma Y, Cheng Y, Beachy PA
Nature (2021) 599 pp. 320-324 [ Pubmed: 34707294 DOI: doi:10.1038/s41586-021-03996-0 ]
- Dispatched protein 't' conformation (151 kDa, Complex from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Sodium ion (22 Da, Ligand)
- Protein dispatched homolog 1 (152 kDa, Protein from Mus musculus)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Water (18 Da, Ligand)
Cryo-EM structure of murine Dispatched NNN mutant
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7rpj
Q-score: 0.427
Wang Q, Asarnow DE, Ding K, Mann RK, Hatakeyama J, Zhang Y, Ma Y, Cheng Y, Beachy PA
Nature (2021) 599 pp. 320-324 [ Pubmed: 34707294 DOI: doi:10.1038/s41586-021-03996-0 ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- Dispatched protein nnn mutant (151 kDa, Complex from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Protein dispatched homolog 1 (152 kDa, Protein from Mus musculus)
Cryo-EM structure of murine Dispatched in complex with Sonic hedgehog
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7rpk
Q-score: 0.547
Wang Q, Asarnow DE, Ding K, Mann RK, Hatakeyama J, Zhang Y, Ma Y, Cheng Y, Beachy PA
Nature (2021) 599 pp. 320-324 [ Pubmed: 34707294 DOI: doi:10.1038/s41586-021-03996-0 ]
- Zinc ion (65 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Sulfate ion (96 Da, Ligand)
- Dispatched protein complexed with sonic hedgehog 26-189 (151 kDa, Complex from Mus musculus)
- Sodium ion (22 Da, Ligand)
- Sonic hedgehog protein (18 kDa, Protein from Mus musculus)
- Protein dispatched homolog 1 (152 kDa, Protein from Mus musculus)
- Cholesterol hemisuccinate (486 Da, Ligand)
- (2s)-3-{[(s)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(hexanoyloxy)propyl hexanoate (411 Da, Ligand)
- Water (18 Da, Ligand)