Influenza A/H7N9 polymerase elongation complex
Resolution: 2.48 Å
EM Method: Single-particle
Fitted PDBs: 7qtl
Q-score: 0.611
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ DOI: doi:10.1016/j.celrep.2022.111901 Pubmed: 36596301 ]
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 3' vrna (6 kDa, RNA from Influenza A virus)
- Water (18 Da, Ligand)
- Polymerase basic protein 2 (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine (481 Da, Ligand)
- 5' vrna (4 kDa, RNA from Influenza A virus)
- Magnesium ion (24 Da, Ligand)
- A/h7n9-elongation (255 kDa, Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Mrna (5 kDa, RNA from Influenza A virus)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
Resolution: 2.51 Å
EM Method: Single-particle
Fitted PDBs: 7r0e
Q-score: 0.538
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ DOI: doi:10.1016/j.celrep.2022.111901 Pubmed: 36596301 ]
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Water (18 Da, Ligand)
- Polymerase basic protein 2 (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 3' vrna (6 kDa, RNA from Influenza A virus)
- 5' vrna (4 kDa, RNA from Influenza A virus)
- Magnesium ion (24 Da, Ligand)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- A/h7n9-backtracked-t/u+1 (255 kDa, Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Mrna (6 kDa, RNA from Influenza A virus)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 7r1f
Q-score: 0.524
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ DOI: doi:10.1016/j.celrep.2022.111901 Pubmed: 36596301 ]
- Water (18 Da, Ligand)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Polymerase basic protein 2 (90 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- 5' vrna (4 kDa, RNA from Influenza B virus)
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- B/mem-backtracked (255 kDa, Complex from Influenza B virus (B/Memphis/13/2003))
- Mrna (6 kDa, RNA from Influenza B virus)
- Magnesium ion (24 Da, Ligand)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- 3' vrna (6 kDa, RNA from Influenza B virus)
Symmetric dimer of influenza A/H7N9 polymerase bound to 5' vRNA hook
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 7zpl
Q-score: 0.481
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ DOI: doi:10.1016/j.celrep.2022.111901 Pubmed: 36596301 ]
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Symmetric dimer of influenza a/h7n9 polymerase bound to 5' vrna hook (510 kDa, Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Magnesium ion (24 Da, Ligand)
- 5' vrna (4 kDa, RNA from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
Influenza A/H7N9 polymerase apo-protein dimer complex
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 7zpm
Q-score: 0.517
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ DOI: doi:10.1016/j.celrep.2022.111901 Pubmed: 36596301 ]
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Influenza a/h7n9 polymerase apo-protein dimer complex (510 kDa, Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8bdr
Q-score: 0.565
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ DOI: doi:10.1016/j.celrep.2022.111901 Pubmed: 36596301 ]
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Polymerase basic protein 2 (90 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- 5' vrna (4 kDa, RNA from Influenza B virus)
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Mrna (5 kDa, RNA from Influenza B virus (B/Kaliningrad/RII06/2012))
- Magnesium ion (24 Da, Ligand)
- Early transcription elongation state of influenza b/mem polymerase backtracked due to double incoproation of nucleotide analogue t1106 and with singly incoporated t1106 at the u +1 position (Complex from Influenza B virus (B/Memphis/13/2003))
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- 3' vrna (6 kDa, RNA from Influenza B virus (B/Acre/117700/2012))
Resolution: 2.34 Å
EM Method: Single-particle
Fitted PDBs: 8be0
Q-score: 0.597
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ DOI: doi:10.1016/j.celrep.2022.111901 Pubmed: 36596301 ]
- Water (18 Da, Ligand)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Polymerase basic protein 2 (90 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- 5' vrna (4 kDa, RNA from Influenza B virus)
- Magnesium ion (24 Da, Ligand)
- Mrna (6 kDa, RNA from Influenza B virus)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- 3' vrna (6 kDa, RNA from Influenza B virus)
- Early transcription elongation state of influenza b/mem polymerase backtracked due to double incoproation of nucleotide analogue t1106 and with singly incoporated t1106 at the c +1 position (Complex from Influenza B virus (B/Memphis/13/2003))
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 8bek
Q-score: 0.556
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ DOI: doi:10.1016/j.celrep.2022.111901 Pubmed: 36596301 ]
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Water (18 Da, Ligand)
- Polymerase basic protein 2 (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 3' vrna (6 kDa, RNA from Influenza A virus)
- 5' vrna (4 kDa, RNA from Influenza A virus)
- Magnesium ion (24 Da, Ligand)
- Mrna (5 kDa, RNA from Influenza A virus)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- A/h7n9-backtracked-t/u+1 complex with only single incorporation (255 kDa, Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8bf5
Q-score: 0.54
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ DOI: doi:10.1016/j.celrep.2022.111901 Pubmed: 36596301 ]
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Polymerase basic protein 2 (90 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- 5' vrna (4 kDa, RNA from Influenza B virus)
- Early transcription elongation state of influenza a/h7n9 polymerase stalled with incoming gtp analogue (255 kDa, Complex from Influenza B virus (B/Memphis/13/2003))
- Magnesium ion (24 Da, Ligand)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- Mrna (5 kDa, RNA from Influenza B virus)
- 3' vrna (6 kDa, RNA from Influenza B virus)