Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 7r1f
Q-score: 0.524
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ DOI: doi:10.1016/j.celrep.2022.111901 Pubmed: 36596301 ]
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Magnesium ion (24 Da, Ligand)
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- 3' vrna (6 kDa, RNA from Influenza B virus)
- Water (18 Da, Ligand)
- 5' vrna (4 kDa, RNA from Influenza B virus)
- Mrna (6 kDa, RNA from Influenza B virus)
- B/mem-backtracked (255 kDa, Complex from Influenza B virus (B/Memphis/13/2003))
- Polymerase basic protein 2 (90 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8bdr
Q-score: 0.565
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Polymerase basic protein 2 (90 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Magnesium ion (24 Da, Ligand)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- 3' vrna (6 kDa, RNA from Influenza B virus (B/Acre/117700/2012))
- Mrna (5 kDa, RNA from Influenza B virus (B/Kaliningrad/RII06/2012))
- Early transcription elongation state of influenza b/mem polymerase backtracked due to double incoproation of nucleotide analogue t1106 and with singly incoporated t1106 at the u +1 position (Complex from Influenza B virus (B/Memphis/13/2003))
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- 5' vrna (4 kDa, RNA from Influenza B virus)
Resolution: 2.34 Å
EM Method: Single-particle
Fitted PDBs: 8be0
Q-score: 0.597
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Polymerase basic protein 2 (90 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- 3' vrna (6 kDa, RNA from Influenza B virus)
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Mrna (6 kDa, RNA from Influenza B virus)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- 5' vrna (4 kDa, RNA from Influenza B virus)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- Early transcription elongation state of influenza b/mem polymerase backtracked due to double incoproation of nucleotide analogue t1106 and with singly incoporated t1106 at the c +1 position (Complex from Influenza B virus (B/Memphis/13/2003))
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8bf5
Q-score: 0.54
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Polymerase basic protein 2 (90 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Magnesium ion (24 Da, Ligand)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- 3' vrna (6 kDa, RNA from Influenza B virus)
- 5' vrna (4 kDa, RNA from Influenza B virus)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- Mrna (5 kDa, RNA from Influenza B virus)
- Early transcription elongation state of influenza a/h7n9 polymerase stalled with incoming gtp analogue (255 kDa, Complex from Influenza B virus (B/Memphis/13/2003))