Electron Microscopy Data Bank

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Cryo-EM map of PEDV (Pintung 52) S protein with all three protomers in the D0-down conformation determined in situ on intact viral particles.

Release date: Aug. 3, 2022
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 7w6m
Q-score: 0.267

Huang CY, Draczkowski P, Wang YS, Chang CY, Chien YC, Cheng YH, Wu YM, Wang CH, Chang YC, Chang YC, Yang TJ, Tsai YX, Khoo KH, Chang HW, Hsu SD

Nat Commun (2022) 13 pp. 4877-4877 [ DOI: doi:10.1038/s41467-022-32588-3 Pubmed: 35986008 ]

Sample:

2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Intact porcine epidemic diarrhea virus (strain pintung 52) (Cellular component from Porcine epidemic diarrhea virus)
Spike glycoprotein (152 kDa, Protein from Porcine epidemic diarrhea virus)

EMD-32332

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Subtomogram averaging of PEDV (Pintung 52) S protein with all three protomers in the D0-down conformation determined in situ on intact viral particles.

Release date: Aug. 3, 2022
Resolution: 19.0 Å
EM Method: Subtomogram averaging

Huang CY, Draczkowski P, Wang YS, Chang CY, Chien YC, Cheng YH, Wu YM, Wang CH, Chang YC, Chang YC, Yang TJ, Tsai YX, Khoo KH, Chang HW, Hsu SD

Nat Commun (2022) 13 pp. 4877-4877 [ DOI: doi:10.1038/s41467-022-32588-3 Pubmed: 35986008 ]

Sample:

Intact porcine epidemic diarrhea virus (strain pintung 52) (Cellular component from Porcine epidemic diarrhea virus)

EMD-32333

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Subtomogram averaging of PEDV (Pintung 52) S protein with one protomer in the D0-up conformation and two protomers in the D0-down conformation, determined in situ on intact viral particles

Release date: Aug. 3, 2022
Resolution: 27.0 Å
EM Method: Subtomogram averaging

Huang CY, Draczkowski P, Wang YS, Chang CY, Chien YC, Cheng YH, Wu YM, Wang CH, Chang YC, Chang YC, Yang TJ, Tsai YX, Khoo KH, Chang HW, Hsu SD

Nat Commun (2022) 13 pp. 4877-4877 [ DOI: doi:10.1038/s41467-022-32588-3 Pubmed: 35986008 ]

Sample:

Intact porcine epidemic diarrhea virus (strain pintung 52) (Cellular component from Porcine epidemic diarrhea virus)

EMD-32337

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Subtomogram averaging of PEDV (Pintung 52) S protein with two protomers in the D0-up conformation and one protomer in the D0-down conformation, determined in situ on intact viral particles.

Release date: Aug. 3, 2022
Resolution: 25.0 Å
EM Method: Subtomogram averaging

Huang CY, Draczkowski P, Wang YS, Chang CY, Chien YC, Cheng YH, Wu YM, Wang CH, Chang YC, Chang YC, Yang TJ, Tsai YX, Khoo KH, Chang HW, Hsu SD

Nat Commun (2022) 13 pp. 4877-4877 [ DOI: doi:10.1038/s41467-022-32588-3 Pubmed: 35986008 ]

Sample:

Intact porcine epidemic diarrhea virus (strain pintung 52) (Cellular component from Porcine epidemic diarrhea virus)

EMD-32338

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Cryo-EM map of PEDV S protein with one protomer in the D0-up conformation while the other two in the D0-down conformation

Release date: Aug. 3, 2022
Resolution: 6.4 Å
EM Method: Single-particle
Fitted PDBs: 7w73
Q-score: 0.229

Huang CY, Draczkowski P, Wang YS, Chang CY, Chien YC, Cheng YH, Wu YM, Wang CH, Chang YC, Chang YC, Yang TJ, Tsai YX, Khoo KH, Chang HW, Hsu SD

Nat Commun (2022) 13 pp. 4877-4877 [ DOI: doi:10.1038/s41467-022-32588-3 Pubmed: 35986008 ]

Sample:

2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Intact porcine epidemic diarrhea virus (strain pintung 52) (Cellular component from Porcine epidemic diarrhea virus)
Spike glycoprotein (152 kDa, Protein from Porcine epidemic diarrhea virus)

EMD-32339

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Subtomogram averaging of PEDV (Pintung 52) S protein with all three protomers in the D0-up conformation determined in situ on intact viral particles.

Release date: Aug. 3, 2022
Resolution: 29.0 Å
EM Method: Subtomogram averaging

Huang CY, Draczkowski P, Wang YS, Chang CY, Chien YC, Cheng YH, Wu YM, Wang CH, Chang YC, Chang YC, Yang TJ, Tsai YX, Khoo KH, Chang HW, Hsu SD

Nat Commun (2022) 13 pp. 4877-4877 [ DOI: doi:10.1038/s41467-022-32588-3 Pubmed: 35986008 ]

Sample:

Intact porcine epidemic diarrhea virus (strain pintung 52) (Cellular component from Porcine epidemic diarrhea virus)

EMD-32340

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Subtomogram averaging of PEDV (Pintung 52) S protein in the postfusion form determined in situ on intact viral particles.

Release date: Aug. 3, 2022
Resolution: 31.0 Å
EM Method: Subtomogram averaging

Huang CY, Draczkowski P, Wang YS, Chang CY, Chien YC, Cheng YH, Wu YM, Wang CH, Chang YC, Chang YC, Yang TJ, Tsai YX, Khoo KH, Chang HW, Hsu SD

Nat Commun (2022) 13 pp. 4877-4877 [ DOI: doi:10.1038/s41467-022-32588-3 Pubmed: 35986008 ]

Sample:

Intact porcine epidemic diarrhea virus (strain pintung 52) (Cellular component from Porcine epidemic diarrhea virus)

EMD-33646

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Cryo-EM map of IPEC-J2 cell-derived PEDV PT52 S protein with three D0-up

Release date: Aug. 3, 2022
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7y6s
Q-score: 0.46

Huang CY, Draczkowski P, Wang YS, Chang CY, Chien YC, Cheng YH, Wu YM, Wang CH, Chang YC, Chang YC, Yang TJ, Tsai YX, Khoo KH, Chang HW, Hsu SD

Nat Commun (2022) 13 pp. 4877-4877 [ DOI: doi:10.1038/s41467-022-32588-3 Pubmed: 35986008 ]

Sample:

Spike glycoprotein (151 kDa, Protein from Porcine epidemic diarrhea virus)
2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Recombinat porcine epidemic diarrhea virus (strain pintung 52) 2p spike (Cellular component from Porcine epidemic diarrhea virus)

EMD-33647

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Cryo-EM map of IPEC-J2 cell-derived PEDV PT52 S protein one D0-down and two D0-up

Release date: Aug. 3, 2022
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7y6t
Q-score: 0.264

Huang CY, Draczkowski P, Wang YS, Chang CY, Chien YC, Cheng YH, Wu YM, Wang CH, Chang YC, Chang YC, Yang TJ, Tsai YX, Khoo KH, Chang HW, Hsu SD

Nat Commun (2022) 13 pp. 4877-4877 [ DOI: doi:10.1038/s41467-022-32588-3 Pubmed: 35986008 ]

Sample:

Spike glycoprotein (153 kDa, Protein from Porcine epidemic diarrhea virus)
2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Recombinant porcine epidemic diarrhea virus (strain pintung 52) 2p spike (Cellular component from Porcine epidemic diarrhea virus)

EMD-33648

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Symmetry-expanded and locally refined protomer structure of IPEC-J2 cell-derived PEDV PT52 S with a CTD-close conformation

Release date: Aug. 3, 2022
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7y6u
Q-score: 0.435

Huang CY, Draczkowski P, Wang YS, Chang CY, Chien YC, Cheng YH, Wu YM, Wang CH, Chang YC, Chang YC, Yang TJ, Tsai YX, Khoo KH, Chang HW, Hsu SD

Nat Commun (2022) 13 pp. 4877-4877 [ DOI: doi:10.1038/s41467-022-32588-3 Pubmed: 35986008 ]

Sample:

Spike glycoprotein (153 kDa, Protein from Porcine epidemic diarrhea virus)
2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Recombinant porcine epidemic diarrhea virus (strain pintung 52) 2p spike (Cellular component from Porcine epidemic diarrhea virus)

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