Influenza A/H7N9 polymerase elongation complex
Resolution: 2.48 Å
EM Method: Single-particle
Fitted PDBs: 7qtl
Q-score: 0.611
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Magnesium ion (24 Da, Ligand)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Water (18 Da, Ligand)
- Polymerase basic protein 2 (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine (481 Da, Ligand)
- A/h7n9-elongation (255 kDa, Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 3' vrna (6 kDa, RNA from Influenza A virus)
- Mrna (5 kDa, RNA from Influenza A virus)
- 5' vrna (4 kDa, RNA from Influenza A virus)
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
Resolution: 2.51 Å
EM Method: Single-particle
Fitted PDBs: 7r0e
Q-score: 0.538
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- A/h7n9-backtracked-t/u+1 (255 kDa, Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Magnesium ion (24 Da, Ligand)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Water (18 Da, Ligand)
- Polymerase basic protein 2 (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 5' vrna (4 kDa, RNA from Influenza A virus)
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 3' vrna (6 kDa, RNA from Influenza A virus)
- Mrna (6 kDa, RNA from Influenza A virus)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 8bek
Q-score: 0.556
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Mrna (5 kDa, RNA from Influenza A virus)
- Magnesium ion (24 Da, Ligand)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Water (18 Da, Ligand)
- Polymerase basic protein 2 (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 5' vrna (4 kDa, RNA from Influenza A virus)
- A/h7n9-backtracked-t/u+1 complex with only single incorporation (255 kDa, Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 3' vrna (6 kDa, RNA from Influenza A virus)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)