Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 6szu
Q-score: 0.665
Wandzik JM, Kouba T, Karuppasamy M, Pflug A, Drncova P, Provaznik J, Azevedo N, Cusack S
Cell (2020) 181 pp. 877- [ DOI: doi:10.1016/j.cell.2020.03.061 Pubmed: 32304664 ]
- Rna-directed rna polymerase catalytic subunit (87 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Water (18 Da, Ligand)
- Pyrophosphate 2- (175 Da, Ligand)
- Polymerase acidic protein (85 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Nucleic acids (Complex from Influenza B virus)
- Vrna (14 kDa, RNA from Influenza B virus)
- Polymerase basic protein 2 (91 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Polymerase (Complex from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Bat influenza a polymerase pre-termination complex with pyrophosphate using 44-mer vrna template with mutated oligo(u) sequence (Complex)
- Magnesium ion (24 Da, Ligand)
- 5-oxidanyl-4-oxidanylidene-1-[(1-pyrrolo[2,3-b]pyridin-1-ylcyclopentyl)methyl]pyridine-3-carboxylic acid (353 Da, Ligand)
- Mrna (3 kDa, RNA from Influenza B virus)
Bat Influenza A polymerase elongation complex with incoming UTP analogue (core + endonuclease only)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 6szv
Q-score: 0.634
Wandzik JM, Kouba T, Karuppasamy M, Pflug A, Drncova P, Provaznik J, Azevedo N, Cusack S
Cell (2020) 181 pp. 877- [ DOI: doi:10.1016/j.cell.2020.03.061 Pubmed: 32304664 ]
- Rna-directed rna polymerase catalytic subunit (87 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Bat influenza a polymerase elongation complex with incoming utp analogue (core + endonuclease only) (Complex)
- Water (18 Da, Ligand)
- 3' vrna (6 kDa, RNA from Influenza B virus)
- Polymerase acidic protein (85 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Nucleic acids (Complex from Influenza B virus)
- Polymerase basic protein 2 (91 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Polymerase (Complex from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Magnesium ion (24 Da, Ligand)
- 5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine (483 Da, Ligand)
- 5-oxidanyl-4-oxidanylidene-1-[(1-pyrrolo[2,3-b]pyridin-1-ylcyclopentyl)methyl]pyridine-3-carboxylic acid (353 Da, Ligand)
- Capped mrna (8 kDa, RNA from Influenza B virus)
- 5' vrna (4 kDa, RNA from Influenza B virus)
Bat Influenza A polymerase pre-initiation complex
Resolution: 2.54 Å
EM Method: Single-particle
Fitted PDBs: 6t0n
Q-score: 0.63
Wandzik JM, Kouba T, Karuppasamy M, Pflug A, Drncova P, Provaznik J, Azevedo N, Cusack S
Cell (2020) 181 pp. 877- [ DOI: doi:10.1016/j.cell.2020.03.061 Pubmed: 32304664 ]
- Rna-directed rna polymerase catalytic subunit (87 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Bat influenza a polymerase pre-initiation complex (Complex)
- Polymerase acidic protein (85 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Nucleic acid (Complex from Influenza B virus)
- 3' vrna (4 kDa, RNA from Influenza B virus)
- Polymerase basic protein 2 (91 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Polymerase (Complex from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- 5' vrna (4 kDa, RNA from Influenza B virus)
Resolution: 2.82 Å
EM Method: Single-particle
Fitted PDBs: 6t0r
Q-score: 0.563
Wandzik JM, Kouba T, Karuppasamy M, Pflug A, Drncova P, Provaznik J, Azevedo N, Cusack S
Cell (2020) 181 pp. 877- [ DOI: doi:10.1016/j.cell.2020.03.061 Pubmed: 32304664 ]
- Rna-directed rna polymerase catalytic subunit (87 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Polymerase acidic protein (85 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Polymerase basic protein 2 (91 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Vrna (14 kDa, RNA from synthetic construct)
- Polymerase (Complex from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Bat influenza a polymerase product dissociation complex using 44-mer vrna template with mutated oligo(u) sequence (Complex)
- Nucleic acid (Complex from synthetic construct)
- Water (18 Da, Ligand)
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 6t0s
Q-score: 0.555
Wandzik JM, Kouba T, Karuppasamy M, Pflug A, Drncova P, Provaznik J, Azevedo N, Cusack S
Cell (2020) 181 pp. 877- [ DOI: doi:10.1016/j.cell.2020.03.061 Pubmed: 32304664 ]
- Rna-directed rna polymerase catalytic subunit (87 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Bat influenza a polymerase stuttering complex using 44-mer vrna template with intact oligo(u) sequence (Complex)
- Polymerase acidic protein (85 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Polymerase basic protein 2 (91 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Mrna (3 kDa, RNA from synthetic construct)
- Polymerase (Complex from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Magnesium ion (24 Da, Ligand)
- Vrna (13 kDa, RNA from synthetic construct)
- Nucleic acid (Complex from synthetic construct)
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 6t0u
Q-score: 0.544
Wandzik JM, Kouba T, Karuppasamy M, Pflug A, Drncova P, Provaznik J, Azevedo N, Cusack S
Cell (2020) 181 pp. 877- [ DOI: doi:10.1016/j.cell.2020.03.061 Pubmed: 32304664 ]
- Rna-directed rna polymerase catalytic subunit (87 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Nucleic acid (Complex from Influenza B virus)
- Polymerase acidic protein (85 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Polymerase basic protein 2 (91 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Polymerase (Complex from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Bat influenza a polymerase product dissociation complex using 44-mer vrna template with intact oligo(u) sequence (Complex)
- Vrna (13 kDa, RNA from Influenza B virus)
Bat Influenza A polymerase elongation complex with incoming UTP analogue (complete polymerase)
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 6t0v
Q-score: 0.552
Wandzik JM, Kouba T, Karuppasamy M, Pflug A, Drncova P, Provaznik J, Azevedo N, Cusack S
Cell (2020) 181 pp. 877- [ DOI: doi:10.1016/j.cell.2020.03.061 Pubmed: 32304664 ]
- Rna-directed rna polymerase catalytic subunit (87 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Polymerase acidic protein (85 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Bat influenza a polymerase elongation complex with incoming utp analogue (complete polymerase) (Complex)
- Nucleic acids (Complex from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Polymerase basic protein 2 (91 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Polymerase (Complex from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- 5' vrna (4 kDa, RNA from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Magnesium ion (24 Da, Ligand)
- 5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine (483 Da, Ligand)
- 3' vrna (6 kDa, RNA from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- 5-oxidanyl-4-oxidanylidene-1-[(1-pyrrolo[2,3-b]pyridin-1-ylcyclopentyl)methyl]pyridine-3-carboxylic acid (353 Da, Ligand)
- Capped mrna (8 kDa, RNA from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
Human Influenza B polymerase recycling complex
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 6t0w
Q-score: 0.546
Wandzik JM, Kouba T, Karuppasamy M, Pflug A, Drncova P, Provaznik J, Azevedo N, Cusack S
Cell (2020) 181 pp. 877- [ DOI: doi:10.1016/j.cell.2020.03.061 Pubmed: 32304664 ]
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus)
- Nucleic acid (Complex from Influenza B virus)
- Polymerase pb2 (90 kDa, Protein from Influenza B virus)
- Vrna (10 kDa, RNA from Influenza B virus)
- Human influenza b polymerase recycling complex (Complex)
- Polymerase (Complex from Influenza B virus)
Bat Influenza A polymerase recycling complex
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 6t2c
Q-score: 0.508
Wandzik JM, Kouba T, Karuppasamy M, Pflug A, Drncova P, Provaznik J, Azevedo N, Cusack S
Cell (2020) 181 pp. 877- [ DOI: doi:10.1016/j.cell.2020.03.061 Pubmed: 32304664 ]
- Rna-directed rna polymerase catalytic subunit (87 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Bat influenza a polymerase recycling complex (Complex)
- Polymerase acidic protein (85 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Nucleic acids (Complex from Influenza B virus)
- Polymerase basic protein 2 (91 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Vrna (10 kDa, RNA from Influenza B virus)
- Polymerase (Complex from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Magnesium ion (24 Da, Ligand)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 6tw1
Q-score: 0.591
Wandzik JM, Kouba T, Karuppasamy M, Pflug A, Drncova P, Provaznik J, Azevedo N, Cusack S
Cell (2020) [ DOI: doi:10.1016/j.cell.2020.03.061 Pubmed: 32304664 ]
- Mrna (3 kDa, RNA from Influenza B virus)
- Rna-directed rna polymerase catalytic subunit (87 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Water (18 Da, Ligand)
- Pyrophosphate 2- (175 Da, Ligand)
- Polymerase acidic protein (85 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Nucleic acids (Complex from Influenza B virus)
- Vrna (14 kDa, RNA from Influenza B virus)
- Polymerase basic protein 2 (91 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Bat influenza a polymerase termination complex with pyrophosphate using 44-mer vrna template with mutated oligo(u) sequence (Complex)
- Polymerase (Complex from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Magnesium ion (24 Da, Ligand)
- 5-oxidanyl-4-oxidanylidene-1-[(1-pyrrolo[2,3-b]pyridin-1-ylcyclopentyl)methyl]pyridine-3-carboxylic acid (353 Da, Ligand)