Influenza A/H7N9 polymerase elongation complex
Resolution: 2.48 Å
EM Method: Single-particle
Fitted PDBs: 7qtl
Q-score: 0.611
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Polymerase basic protein 2 (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine (481 Da, Ligand)
- 3' vrna (6 kDa, RNA from Influenza A virus)
- 5' vrna (4 kDa, RNA from Influenza A virus)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- A/h7n9-elongation (255 kDa, Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Mrna (5 kDa, RNA from Influenza A virus)
- Magnesium ion (24 Da, Ligand)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Water (18 Da, Ligand)
Resolution: 2.51 Å
EM Method: Single-particle
Fitted PDBs: 7r0e
Q-score: 0.538
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Polymerase basic protein 2 (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- A/h7n9-backtracked-t/u+1 (255 kDa, Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Mrna (6 kDa, RNA from Influenza A virus)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 5' vrna (4 kDa, RNA from Influenza A virus)
- Magnesium ion (24 Da, Ligand)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 3' vrna (6 kDa, RNA from Influenza A virus)
- Water (18 Da, Ligand)
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 7r1f
Q-score: 0.524
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- B/mem-backtracked (255 kDa, Complex from Influenza B virus (B/Memphis/13/2003))
- 3' vrna (6 kDa, RNA from Influenza B virus)
- 5' vrna (4 kDa, RNA from Influenza B virus)
- Mrna (6 kDa, RNA from Influenza B virus)
- Magnesium ion (24 Da, Ligand)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- Water (18 Da, Ligand)
- Polymerase basic protein 2 (90 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8bdr
Q-score: 0.565
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Early transcription elongation state of influenza b/mem polymerase backtracked due to double incoproation of nucleotide analogue t1106 and with singly incoporated t1106 at the u +1 position (Complex from Influenza B virus (B/Memphis/13/2003))
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Mrna (5 kDa, RNA from Influenza B virus (B/Kaliningrad/RII06/2012))
- 5' vrna (4 kDa, RNA from Influenza B virus)
- Magnesium ion (24 Da, Ligand)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- 3' vrna (6 kDa, RNA from Influenza B virus (B/Acre/117700/2012))
- Polymerase basic protein 2 (90 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
Resolution: 2.34 Å
EM Method: Single-particle
Fitted PDBs: 8be0
Q-score: 0.597
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Early transcription elongation state of influenza b/mem polymerase backtracked due to double incoproation of nucleotide analogue t1106 and with singly incoporated t1106 at the c +1 position (Complex from Influenza B virus (B/Memphis/13/2003))
- 5' vrna (4 kDa, RNA from Influenza B virus)
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Magnesium ion (24 Da, Ligand)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- 3' vrna (6 kDa, RNA from Influenza B virus)
- Mrna (6 kDa, RNA from Influenza B virus)
- Water (18 Da, Ligand)
- Polymerase basic protein 2 (90 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 8bek
Q-score: 0.556
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Polymerase basic protein 2 (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- A/h7n9-backtracked-t/u+1 complex with only single incorporation (255 kDa, Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Mrna (5 kDa, RNA from Influenza A virus)
- 5' vrna (4 kDa, RNA from Influenza A virus)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Magnesium ion (24 Da, Ligand)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 3' vrna (6 kDa, RNA from Influenza A virus)
- Water (18 Da, Ligand)
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8bf5
Q-score: 0.54
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- 5' vrna (4 kDa, RNA from Influenza B virus)
- Mrna (5 kDa, RNA from Influenza B virus)
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- 3' vrna (6 kDa, RNA from Influenza B virus)
- Early transcription elongation state of influenza a/h7n9 polymerase stalled with incoming gtp analogue (255 kDa, Complex from Influenza B virus (B/Memphis/13/2003))
- Magnesium ion (24 Da, Ligand)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- Polymerase basic protein 2 (90 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 6szu
Q-score: 0.665
Wandzik JM, Kouba T, Karuppasamy M, Pflug A, Drncova P, Provaznik J, Azevedo N, Cusack S
Cell (2020) 181 pp. 877- [ DOI: doi:10.1016/j.cell.2020.03.061 Pubmed: 32304664 ]
- Rna-directed rna polymerase catalytic subunit (87 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Water (18 Da, Ligand)
- Pyrophosphate 2- (175 Da, Ligand)
- Polymerase acidic protein (85 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Nucleic acids (Complex from Influenza B virus)
- Vrna (14 kDa, RNA from Influenza B virus)
- Polymerase basic protein 2 (91 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Polymerase (Complex from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Bat influenza a polymerase pre-termination complex with pyrophosphate using 44-mer vrna template with mutated oligo(u) sequence (Complex)
- Magnesium ion (24 Da, Ligand)
- 5-oxidanyl-4-oxidanylidene-1-[(1-pyrrolo[2,3-b]pyridin-1-ylcyclopentyl)methyl]pyridine-3-carboxylic acid (353 Da, Ligand)
- Mrna (3 kDa, RNA from Influenza B virus)
Bat Influenza A polymerase elongation complex with incoming UTP analogue (core + endonuclease only)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 6szv
Q-score: 0.634
Wandzik JM, Kouba T, Karuppasamy M, Pflug A, Drncova P, Provaznik J, Azevedo N, Cusack S
Cell (2020) 181 pp. 877- [ DOI: doi:10.1016/j.cell.2020.03.061 Pubmed: 32304664 ]
- Rna-directed rna polymerase catalytic subunit (87 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Bat influenza a polymerase elongation complex with incoming utp analogue (core + endonuclease only) (Complex)
- Water (18 Da, Ligand)
- 3' vrna (6 kDa, RNA from Influenza B virus)
- Polymerase acidic protein (85 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Nucleic acids (Complex from Influenza B virus)
- Polymerase basic protein 2 (91 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Polymerase (Complex from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Magnesium ion (24 Da, Ligand)
- 5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine (483 Da, Ligand)
- 5-oxidanyl-4-oxidanylidene-1-[(1-pyrrolo[2,3-b]pyridin-1-ylcyclopentyl)methyl]pyridine-3-carboxylic acid (353 Da, Ligand)
- Capped mrna (8 kDa, RNA from Influenza B virus)
- 5' vrna (4 kDa, RNA from Influenza B virus)
Bat Influenza A polymerase pre-initiation complex
Resolution: 2.54 Å
EM Method: Single-particle
Fitted PDBs: 6t0n
Q-score: 0.63
Wandzik JM, Kouba T, Karuppasamy M, Pflug A, Drncova P, Provaznik J, Azevedo N, Cusack S
Cell (2020) 181 pp. 877- [ DOI: doi:10.1016/j.cell.2020.03.061 Pubmed: 32304664 ]
- Rna-directed rna polymerase catalytic subunit (87 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Bat influenza a polymerase pre-initiation complex (Complex)
- Polymerase acidic protein (85 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Nucleic acid (Complex from Influenza B virus)
- 3' vrna (4 kDa, RNA from Influenza B virus)
- Polymerase basic protein 2 (91 kDa, Protein from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Polymerase (Complex from Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10)))
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- 5' vrna (4 kDa, RNA from Influenza B virus)