CryoEM structure of CENP-N promoted nucleosome stacks with CENP-A and 601 DNA sequence
Resolution: 8.1 Å
EM Method: Single-particle
Fitted PDBs: 7u4d
Q-score: 0.114
Zhou K, Gebala M, Woods D, Sundararajan K, Edwards G, Krzizike D, Wereszczynski J, Straight AF, Luger K
Nat Struct Mol Biol (2022) 29 pp. 403-413 [ Pubmed: 35422519 DOI: doi:10.1038/s41594-022-00758-y ]
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Histone h3-like centromeric protein a (16 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (147-mer) (45 kDa, DNA from Escherichia coli)
- Dna (147-mer) (45 kDa, DNA from Escherichia coli)
- Centromere protein n (34 kDa, Protein from Homo sapiens)
- Human kinetochore protein cenp-n with the centromeric nucleosome containing cenp-a (601 sequence dna) (Complex from Homo sapiens)
Cryo-EM structure of centromeric chromatin array shaped by the kinetochore protein CENP-N
Zhou K, Gebala M, Woods D, Sundararajan K, Edwards G, Krzizike D, Wereszczynski J, Straight AF, Luger K
Nat Struct Mol Biol (2022) 29 pp. 403-413 [ Pubmed: 35422519 DOI: doi:10.1038/s41594-022-00758-y ]
Cryo-EM structure of CENP-A nucleosome (palindromic alpha satellite DNA) in complex with CENP-N
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 7u46
Q-score: 0.559
Zhou K, Gebala M, Woods D, Sundararajan K, Edwards G, Krzizike D, Wereszczynski J, Straight AF, Luger K
Nat Struct Mol Biol (2022) 29 pp. 403-413 [ Pubmed: 35422519 DOI: doi:10.1038/s41594-022-00758-y ]
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Histone h3-like centromeric protein a (16 kDa, Protein from Homo sapiens)
- Dna (147-mer) (45 kDa, DNA from Escherichia coli)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (147-mer) (45 kDa, DNA from Escherichia coli)
- Centromere protein n (34 kDa, Protein from Homo sapiens)
- Cenp-a nucleosome with kinetochore protein cenp-n (Complex from Homo sapiens)
Resolution: 7.5 Å
EM Method: Single-particle
Fitted PDBs: 7u47
Q-score: 0.179
Zhou K, Gebala M, Woods D, Sundararajan K, Edwards G, Krzizike D, Wereszczynski J, Straight AF, Luger K
Nat Struct Mol Biol (2022) 29 pp. 403-413 [ Pubmed: 35422519 DOI: doi:10.1038/s41594-022-00758-y ]
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Cryoem structure of centromeric nucleosome in complex with kinetochore protein cenp-n (Complex from Homo sapiens)
- Histone h3-like centromeric protein a (16 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (147-mer) (45 kDa, DNA from Escherichia coli)
- Dna (147-mer) (45 kDa, DNA from Escherichia coli)
- Centromere protein n (34 kDa, Protein from Homo sapiens)
Melbournevirus nucleosome like particle
Resolution: 3.89 Å
EM Method: Single-particle
Fitted PDBs: 7n8n
Q-score: 0.268
Liu Y, Bisio H, Toner CM, Jeudy S, Philippe N, Zhou K, Bowerman S, White A, Edwards G, Abergel C, Luger K
Cell (2021) 184 pp. 4237-4250.e19 [ DOI: doi:10.1016/j.cell.2021.06.032 Pubmed: 34297924 ]
- Histone h2b-h2a doublet (32 kDa, Protein from Melbournevirus)
- Dna (147-mer) (45 kDa, DNA from Escherichia coli)
- Histone h4-h3 doublet (26 kDa, Protein from Melbournevirus)
- Dna 147-mer (Complex from Escherichia coli)
- Histone h4-h3 doublet, histone h2b-h2a doublet (Complex from Melbournevirus)
- Dna (147-mer) (45 kDa, DNA from Escherichia coli)
- Melbournevirus nucleosome like particle (218 kDa, Complex)