Structure of RyR1 in lipid nanodisc in primed state in complex with nanobody and FKBP12
Li C, Willegems K, Uchanski T, Pardon E, Steyaert J, Efremov RG
J Biol Chem (2024) 300 pp. 107734-107734 [ Pubmed: 39233227 DOI: doi:10.1016/j.jbc.2024.107734 ]
Li C, Willegems K, Uchanski T, Pardon E, Steyaert J, Efremov RG
J Biol Chem (2024) 300 pp. 107734-107734 [ Pubmed: 39233227 DOI: doi:10.1016/j.jbc.2024.107734 ]
Structure of human full-length ancestral TRPV6 channel in apo open state
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 9cuh
Q-score: 0.56
Neuberger A, Shalygin A, Trofimov YA, Veretenenko II, Nadezhdin KD, Krylov NA, Gudermann T, Efremov RG, Chubanov V, Sobolevsky AI
Structure (2025) 33 pp. 91-103.e5 [ DOI: doi:10.1016/j.str.2024.10.018 Pubmed: 39500315 ]
- Calcium ion (40 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Ancestral htrpv6 tetramer in open apo state (370 kDa, Complex from Homo sapiens)
- Transient receptor potential cation channel subfamily v member 6 (87 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Water (18 Da, Ligand)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
Structure of human full-length ancestral TRPV6 channel in Calmodulin-bound state
Resolution: 3.42 Å
EM Method: Single-particle
Fitted PDBs: 9cui
Q-score: 0.468
Neuberger A, Shalygin A, Trofimov YA, Veretenenko II, Nadezhdin KD, Krylov NA, Gudermann T, Efremov RG, Chubanov V, Sobolevsky AI
Structure (2025) 33 pp. 91-103.e5 [ DOI: doi:10.1016/j.str.2024.10.018 Pubmed: 39500315 ]
- Ancestral htrpv6 tetramer in complex with calmodulin (Complex from Homo sapiens)
- Calmodulin-1 (16 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Transient receptor potential cation channel subfamily v member 6 (87 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Cholesterol (386 Da, Ligand)
Structure of human full-length derived TRPV6 channel in apo open state
Resolution: 2.78 Å
EM Method: Single-particle
Fitted PDBs: 9cuj
Q-score: 0.569
Neuberger A, Shalygin A, Trofimov YA, Veretenenko II, Nadezhdin KD, Krylov NA, Gudermann T, Efremov RG, Chubanov V, Sobolevsky AI
Structure (2025) 33 pp. 91-103.e5 [ DOI: doi:10.1016/j.str.2024.10.018 Pubmed: 39500315 ]
- Calcium ion (40 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Derived htrpv6 tetramer in open apo state (370 kDa, Complex from Homo sapiens)
- Transient receptor potential cation channel subfamily v member 6 (87 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
Structure of human full-length derived TRPV6 channel in Calmodulin-bound state
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 9cuk
Q-score: 0.482
Neuberger A, Shalygin A, Trofimov YA, Veretenenko II, Nadezhdin KD, Krylov NA, Gudermann T, Efremov RG, Chubanov V, Sobolevsky AI
Structure (2025) 33 pp. 91-103.e5 [ DOI: doi:10.1016/j.str.2024.10.018 Pubmed: 39500315 ]
- Calmodulin-1 (16 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Derived htrpv6 tetramer in complex with calmodulin (370 kDa, Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Transient receptor potential cation channel subfamily v member 6 (87 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Cholesterol (386 Da, Ligand)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8rrs
Q-score: 0.307
Li C, Willegems K, Uchanski T, Pardon E, Steyaert J, Efremov RG
J Biol Chem (2024) 300 pp. 107734-107734 [ Pubmed: 39233227 DOI: doi:10.1016/j.jbc.2024.107734 ]
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Nanobody 9657 (15 kDa, Protein from Vicugna pacos)
- Ryanodine receptor 2 (565 kDa, Protein from Mus musculus)
- Mouse ryanodine receptor 2 (Complex from Mus musculus)
- Caffeine (194 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Mouse ryanodine receptor 2 complex with nanobody (Complex)
- Nanobody 9657 (Complex from Vicugna pacos)
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 8rrt
Q-score: 0.154
Li C, Willegems K, Uchanski T, Pardon E, Steyaert J, Efremov RG
J Biol Chem (2024) 300 pp. 107734-107734 [ Pubmed: 39233227 DOI: doi:10.1016/j.jbc.2024.107734 ]
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Nanobody 9657 (15 kDa, Protein from Vicugna pacos)
- Ryanodine receptor 1 complex with fkbp12 (Complex from Oryctolagus cuniculus)
- Caffeine (194 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Ryanodine receptor1 complex with nanobody 9657 and fkbp12 (Complex)
- Ryanodine receptor 1 (564 kDa, Protein from Oryctolagus cuniculus)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Oryctolagus cuniculus)
- Nanobody 9657 (Complex from Vicugna pacos)
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 8rru
Q-score: 0.11
Li C, Willegems K, Uchanski T, Pardon E, Steyaert J, Efremov RG
J Biol Chem (2024) 300 pp. 107734-107734 [ Pubmed: 39233227 DOI: doi:10.1016/j.jbc.2024.107734 ]
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Nanobody 9657 (15 kDa, Protein from Vicugna pacos)
- Caffeine (194 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Ryanodine receptor 1 (564 kDa, Protein from Oryctolagus cuniculus)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Oryctolagus cuniculus)
- Ryanodine receptor 1 in complex with fkbp12 (Complex from Oryctolagus cuniculus)
- Ryanodine receptor 1 in complex with nanobody and fkbp12 (Complex)
- Nanobody 9657 (Complex from Vicugna pacos)
Structure of RyR1 in detergent in close state in complex with FKBP and Nb9657.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8rrv
Q-score: 0.38
Li C, Willegems K, Uchanski T, Pardon E, Steyaert J, Efremov RG
J Biol Chem (2024) 300 pp. 107734-107734 [ Pubmed: 39233227 DOI: doi:10.1016/j.jbc.2024.107734 ]
- Ryanodine receptor 1 complex with nanobody and fkbp12 (Complex from Oryctolagus cuniculus)
- Ryanodine receptor 1 (565 kDa, Protein from Oryctolagus cuniculus)
- Zinc ion (65 Da, Ligand)
- Nanobody9657 (15 kDa, Protein from Vicugna pacos)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Oryctolagus cuniculus)