Riggi MR, Antao NV, Lam C, Davydov A, Sall J, Petzold C, Liang F, Iwasa J, Ekiert DC, Bhabha G
bioRxiv (2023) [ DOI: 10.1101/2023.07.02.547383 Pubmed: 37425741 ]
Image sets:
- Uninfected cell 1
- 48h infected cell 5
- 24h infected cell 1
- 48h infected cell 1
- 48h infected cell 3
- 24h infected cell 2
- 48h infected cell 2
- Uninfected cell 2
- 48h infected cell 4
- Purified spores dataset 2
- Purified spores dataset 1
MacRae MR, Puvanendran D, Haase MAB, Coudray N, Kolich L, Lam C, Baek M, Bhabha G, Ekiert DC
J Biol Chem (2023) 299 pp. 104744-104744 [ Pubmed: 37100290 DOI: doi:10.1016/j.jbc.2023.104744 ]
- E. coli mlac bound to mlafedb (Complex from Escherichia coli K-12)
- Mlaf (Protein from Escherichia coli K-12)
- Mlab (Protein from Escherichia coli K-12)
- Foldon-mlac (Protein from Escherichia coli K-12)
- Mlad (Protein from Escherichia coli K-12)
- Mlae (Protein from Escherichia coli K-12)
Single particle cryo-EM dataset for E. coli MlaFEDB bound to MlaC
MacRae MR, Puvanendran D, Haase MAB, Coudray N, Kolich L, Lam C, Baek M, Bhabha G, Ekiert DC
J Biol Chem (2023) [ DOI: 10.1016/j.jbc.2023.104744 Pubmed: 37100290 ]
Image sets:
- unaligned frames of MlaFEDB & MlaC complex (tilted dataset)
- Aligned frames of MlaFEDB & MlaC complex (tilted dataset)
- Aligned frames of MlaFEDB & MlaC complex (untilted dataset)
- Unaligned frames of MlaFEDB & MlaC complex (untilted dataset)
MacRae MR, Puvanendran D, Haase MAB, Coudray N, Kolich L, Lam C, Baek M, Bhabha G, Ekiert DC
J Biol Chem (2023) 299 pp. 104744-104744 [ Pubmed: 37100290 DOI: doi:10.1016/j.jbc.2023.104744 ]
- E. coli mlafedb bound to mlac (Complex from Escherichia coli K-12)
- Mlaf (Protein from Escherichia coli K-12)
- Mlab (Protein from Escherichia coli K-12)
- Mlad (Protein from Escherichia coli K-12)
- Mlac-foldon (Protein from Escherichia coli K-12)
- Mlae (Protein from Escherichia coli K-12)
Cryo-EM structure of MlaFEDB in nanodiscs with phospholipid substrates
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 6xbd
Q-score: 0.489
Coudray N, Isom GL, MacRae MR, Saiduddin MN, Bhabha G, Ekiert DC
eLife (2020) 9 [ DOI: doi:10.7554/eLife.62518 Pubmed: 33236984 ]
- Mlafedb complex with two bound phospholipid substrates in msp1d1 nanodisc (266 kDa, Complex from Escherichia coli K-12)
- Phospholipid abc transporter-binding protein mlab (10 kDa, Protein from Escherichia coli K-12)
- Msp1d1 (13 kDa, Protein from Homo sapiens)
- Phospholipid abc transporter-binding protein mlad (21 kDa, Protein from Escherichia coli DEC6A)
- Phospholipid abc transporter permease protein mlae (27 kDa, Protein from Escherichia coli DEC6A)
- Di-palmitoyl-3-sn-phosphatidylethanolamine (691 Da, Ligand)
- Phospholipid transport system atp-binding protein mlaf (29 kDa, Protein from Escherichia coli DEC6A)
Single particle cryo-EM dataset for LetB from E.coli
Isom GL, Coudray N, MacRae MR, McManus CT, Ekiert DC, Bhabha G
Cell (2020) 181 pp. 653-664.e19 [ DOI: 10.1016/j.cell.2020.03.030 Pubmed: 32359438 ]
Image sets:
- Particle stacks for Model 7 (EMD-20999)
- Particle stacks for Model 6 (EMD-20998)
- Particle stacks for Model 2 (EMD-20994)
- Particle stacks for Model 5 (EMD-20997)
- Aligned, dose-weighted and non-dose-weighted micrographs
- Particle stacks for Model 4 (EMD-20996)
- Particle stacks for Model 8 (EMD-21000)
- Particle stacks for Model 3 (EMD-20995)
- Particle stacks for Model 1 (EMD-20993)
Lipophilic Envelope-spanning Tunnel B (LetB), Map 1
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 6v0c
Q-score: 0.46
Isom GL, Coudray N, MacRae MR, McManus CT, Ekiert DC, Bhabha G
Cell (2020) 181 pp. 653-664.e19 [ DOI: doi:10.1016/j.cell.2020.03.030 Pubmed: 32359438 ]
Lipophilic Envelope-spanning Tunnel B (LetB), Map 2
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 6v0d
Q-score: 0.424
Isom GL, Coudray N, MacRae MR, McManus CT, Ekiert DC, Bhabha G
Cell (2020) 181 pp. 653-664.e19 [ DOI: doi:10.1016/j.cell.2020.03.030 Pubmed: 32359438 ]
Lipophilic Envelope-spanning Tunnel B (LetB), Map 3
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 6v0e
Q-score: 0.501
Isom GL, Coudray N, MacRae MR, McManus CT, Ekiert DC, Bhabha G
Cell (2020) 181 pp. 653-664.e19 [ DOI: doi:10.1016/j.cell.2020.03.030 Pubmed: 32359438 ]
Lipophilic Envelope-spanning Tunnel B (LetB), Map 4
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 6v0f
Q-score: 0.486
Isom GL, Coudray N, MacRae MR, McManus CT, Ekiert DC, Bhabha G
Cell (2020) 181 pp. 653-664.e19 [ DOI: doi:10.1016/j.cell.2020.03.030 Pubmed: 32359438 ]