Electron Microscopy Data Bank

EMD-45671

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Subtomogram average of the Polar Tube Outer Filament layer from Encephalitozoon intestinalis microsporidian spores

Release date: Feb. 26, 2025
Resolution: 36.0 Å
EM Method: Subtomogram averaging

Usmani M, Coudray N, Riggi M, Raghu R, Ramchandani H, Bobe D, Kopylov M, Zhong ED, Iwasa JH, Ekiert DC, Bhabha G

To Be Published

Sample:

Encephalitozoon intestinalis microsporidian spores (Cell from Encephalitozoon intestinalis)

EMD-45672

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Subtomogram average of the Polar Tube Inner Filament layer from Encephalitozoon intestinalis microsporidian spores

Release date: Feb. 26, 2025
Resolution: 20.0 Å
EM Method: Subtomogram averaging

Usmani M, Coudray N, Riggi M, Raghu R, Ramchandani H, Bobe D, Kopylov M, Zhong ED, Iwasa JH, Ekiert DC, Bhabha G

To Be Published

Sample:

Encephalitozoon intestinalis microsporidian spores (Cell from Encephalitozoon intestinalis)

EMD-45673

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Subtomogram average of the Polar Tube Outer Filament Layer and Inner Filament layer from Encephalitozoon intestinalis microsporidian spores

Release date: Feb. 26, 2025
Resolution: 44.0 Å
EM Method: Subtomogram averaging

Usmani M, Coudray N, Riggi M, Raghu R, Ramchandani H, Bobe D, Kopylov M, Zhong ED, Iwasa JH, Ekiert DC, Bhabha G

To Be Published

Sample:

Encephalitozoon intestinalis microsporidian spores (Cell from Encephalitozoon intestinalis)

EMD-45674

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Subtomogram average of a whole Polar Tube cross-section from Encephalitozoon intestinalis microsporidian spores

Release date: Feb. 26, 2025
Resolution: 70.0 Å
EM Method: Subtomogram averaging

Usmani M, Coudray N, Riggi M, Raghu R, Ramchandani H, Bobe D, Kopylov M, Zhong ED, Iwasa JH, Ekiert DC, Bhabha G

To Be Published

Sample:

Encephalitozoon intestinalis microsporidian spores (Cell from Encephalitozoon intestinalis)

EMPIAR-12011

Release date: Dec. 6, 2024
Dataset size: 3.0 TB
Experiment type: EMDB

Kahne SC, Yoo JH, Chen J, Nakedi K, Iyer LM, Putzel G, Samhadaneh NM, Pironti A, Aravind L, Ekiert DC, Bhabha G, Rhee KY, Darwin H

To Be Published

Image sets:

Unaligned multi-frame super-resolution micrographs of M. tuberculosis CoaX

EMPIAR-11683

3D reconstructions of parasite development and the intracellular niche of the microsporidian pathogen E. intestinalis

Release date: Oct. 17, 2023
Dataset size: 537.9 GB
Experiment type: SBF-SEM

Riggi MR, Antao NV, Lam C, Davydov A, Sall J, Petzold C, Liang F, Iwasa J, Ekiert DC, Bhabha G

bioRxiv (2023) [ DOI: 10.1101/2023.07.02.547383 Pubmed: 37425741 ]

Image sets:

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EMD-29023

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Structure of Mce1-LucB complex from Mycobacterium smegmatis (Map1)

Release date: Feb. 22, 2023
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8fed
Q-score: 0.524

Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC

Nature (2023) 620 pp. 445-452 [ Pubmed: 37495693 DOI: doi:10.1038/s41586-023-06366-0 ]

Sample:

Abc-transporter integral membrane protein (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (58 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (37 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (42 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Conserved hypothetical integral membrane protein yrbe1a (27 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Mce-family protein mce1f (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (43 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Unknown ligand (Ligand)
Mce-family protein mce1c (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Transmembrane protein (20 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Abc transporter, atp-binding protein,green fluorescent protein chimera (71 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Mce1 lipid transporter composed of mce1 mce proteins (mce1abcdef) and an abc transporter (yrbe1a-b, 2 copies of mceg) bound to accessory factor lucb (Complex from Mycolicibacterium smegmatis MC2 155)

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EMD-29024

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Structure of Mce1 transporter from Mycobacterium smegmatis in the absence of LucB (Map2)

Release date: Feb. 22, 2023
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8fee
Q-score: 0.502

Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC

Nature (2023) 620 pp. 445-452 [ Pubmed: 37495693 DOI: doi:10.1038/s41586-023-06366-0 ]

Sample:

Abc-transporter integral membrane protein (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (58 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (37 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (42 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Conserved hypothetical integral membrane protein yrbe1a (27 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Mce1 lipid transporter composed of mce1 mce proteins (mce1abcdef) and an abc transporter (yrbe1a-b, 2 copies of mceg) (Complex from Mycolicibacterium smegmatis MC2 155)
Mce-family protein mce1f (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (43 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Unknown ligand (Ligand)
Mce-family protein mce1c (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Abc transporter, atp-binding protein,green fluorescent protein chimera (71 kDa, Protein from Mycolicibacterium smegmatis MC2 155)

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EMD-29025

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Structure of Mce1 transporter from Mycobacterium smegmatis (Map0)

Release date: Feb. 22, 2023
Resolution: 2.71 Å
EM Method: Single-particle
Fitted PDBs: 8fef
Q-score: 0.564

Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC

Nature (2023) 620 pp. 445-452 [ Pubmed: 37495693 DOI: doi:10.1038/s41586-023-06366-0 ]

Sample:

Abc-transporter integral membrane protein (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (58 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (37 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (42 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Conserved hypothetical integral membrane protein yrbe1a (27 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Mce1 lipid transporter composed of mce1 mce proteins (mce1abcdef) and an abc transporter (yrbe1a-b, 2 copies of mceg) (Complex from Mycolicibacterium smegmatis MC2 155)
Mce-family protein mce1f (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (43 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Unknown ligand (Ligand)
Mce-family protein mce1c (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Abc transporter, atp-binding protein,green fluorescent protein chimera (71 kDa, Protein from Mycolicibacterium smegmatis MC2 155)

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EMD-29228

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Local refinement of MceG in Msmeg Mce1 transporter (Map0a)

Release date: Feb. 15, 2023
Resolution: 2.91 Å
EM Method: Single-particle

Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC

Nature (2023) 620 pp. 445-452 [ Pubmed: 37495693 DOI: doi:10.1038/s41586-023-06366-0 ]

Sample:

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