E. coli BAM complex (BamABCDE) bound to darobactin B
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8bvq
Q-score: 0.444
Haysom SF, Machin J, Whitehouse JM, Horne JE, Fenn K, Ma Y, El Mkami H, Bohringer N, Schaberle TF, Ranson NA, Radford SE, Pliotas C
Angew Chem Int Ed Engl (2023) 62 pp. e202218783-e202218783 [ Pubmed: 37162386 DOI: doi:10.1002/anie.202218783 ]
- Outer membrane protein assembly factor bama (88 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamc (34 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamd (25 kDa, Protein from Escherichia coli)
- Darobactin-b (1 kDa, Protein from Photorhabdus heterorhabditis)
- Outer membrane protein assembly factor bamb (39 kDa, Protein from Escherichia coli)
- E. coli bam complex (bamabcde) bound to darobactin b (200 kDa, Complex from Escherichia coli)
- Outer membrane protein assembly factor bame (11 kDa, Protein from Escherichia coli)
E. coli BAM complex (BamABCDE) wild-type
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8bwc
Q-score: 0.482
Haysom SF, Machin J, Whitehouse JM, Horne JE, Fenn K, Ma Y, El Mkami H, Bohringer N, Schaberle TF, Ranson NA, Radford SE, Pliotas C
Angew Chem Int Ed Engl (2023) 62 pp. e202218783-e202218783 [ Pubmed: 37162386 DOI: doi:10.1002/anie.202218783 ]
- Outer membrane protein assembly factor bama (88 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamb (39 kDa, Protein from Escherichia coli BL21(DE3))
- Outer membrane protein assembly factor bama, bamc, bamd and bame (Complex from Escherichia coli)
- Outer membrane protein assembly factor bamc (34 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamd (25 kDa, Protein from Escherichia coli)
- E. coli bam complex (bamabcde) wild-type (200 kDa, Complex)
- Outer membrane protein assembly factor bamb (Complex from Escherichia coli BL21(DE3))
- Outer membrane protein assembly factor bame (11 kDa, Protein from Escherichia coli)
Structure of two molecules of the chromatin remodelling enzyme Chd1 bound to a nucleosome
Resolution: 10.0 Å
EM Method: Single-particle
Fitted PDBs: 6g0l
Q-score: 0.095
Sundaramoorthy R, Hughes AL, El-Mkami H, Norman DG, Ferreira H, Owen-Hughes T
eLife (2018) 7 [ DOI: doi:10.7554/eLife.35720 Pubmed: 30079888 ]
- X. laevis nucleosome pn 601 dna with s.cerevisiae remodeller chd1 (Complex from synthetic construct)
- Beryllium trifluoride ion (66 Da, Ligand)
- Histone h4 (13 kDa, Protein from Xenopus laevis)
- Chromo domain-containing protein 1 (168 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- X. laevis nucleosome pn 601 dna with s.cerevisiae remodeller chd1 (Complex from Saccharomyces cerevisiae S288C)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- Dna (176-mer) (54 kDa, DNA from synthetic construct)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- X. laevis nucleosome pn 601 dna with s.cerevisiae remodeller chd1 (600 kDa, Complex)
- Dna (177-mer) (54 kDa, DNA from synthetic construct)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- X. laevis nucleosome pn 601 dna with s.cerevisiae remodeller chd1 (Complex from Xenopus laevis)
Structure of the chromatin remodelling enzyme Chd1 bound to a ubiquitinylated nucleosome
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6ftx
Q-score: 0.23
Sundaramoorthy R, Hughes AL, El-Mkami H, Norman DG, Ferreira H, Owen-Hughes T
eLife (2018) 7 [ DOI: doi:10.7554/eLife.35720 Pubmed: 30079888 ]
- X. laevis nucleosome pn 601 dna with s.cerevisiae remodeller chd1 (Complex from synthetic construct)
- Dna (159-mer) (48 kDa, DNA from synthetic construct)
- Beryllium trifluoride ion (66 Da, Ligand)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- X. laevis nucleosome pn 601 dna with s.cerevisiae remodeller chd1 (Complex from Saccharomyces cerevisiae)
- Polyubiquitin-b (8 kDa, Protein from Homo sapiens)
- Histone h2b (13 kDa, Protein from Xenopus tropicalis)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- X. laevis nucleosome pn 601 dna with s.cerevisiae remodeller chd1 (Complex from Homo sapiens)
- Dna (160-mer) (49 kDa, DNA from synthetic construct)
- Histone h3 (11 kDa, Protein from Petromyzon marinus)
- Chromatin-remodeling atpase (102 kDa, Protein from Saccharomyces cerevisiae)
- Histone h3.3c (12 kDa, Protein from Xenopus laevis)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- X. laevis nucleosome pn 601 dna with s.cerevisiae remodeller chd1 (Complex from Xenopus laevis)
- X. laevis nucleosome pn 601 dna with s.cerevisiae remodeller chd1 (450 kDa, Complex)
Cryo EM structure of the conjugative relaxes TraI of the F/R1 plasmid system
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5n8o
Q-score: 0.4
Ilangovan A, Kay CWM, Roier S, El Mkami H, Salvadori E, Zechner EL, Zanetti G, Waksman G
Cell (2017) 169 pp. 708-721.e12 [ Pubmed: 28457609 DOI: doi:10.1016/j.cell.2017.04.010 ]
- 22-mer dna/rna hybrid (Complex from Escherichia coli)
- Trai protein (Complex from Escherichia coli)
- Dna (5'-d(p*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*t)-3') (6 kDa, DNA from Escherichia coli)
- Trai-22mer complex (193 kDa, Complex)
- Dna helicase i (191 kDa, Protein from Escherichia coli)
Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes
Sundaramoorthy R, Hughes AL, Singh V, Wiechens N, Ryan DP, El-Mkami H, Petoukhov M, Svergun DI, Treutlein B, Quack S, Fischer M, Michaelis J, Bottcher B, Norman DG, Owen-Hughes T
eLife (2017) 6 [ Pubmed: 28332978 DOI: doi:10.7554/eLife.22510 ]
- Chd1-nucleosome complex (400 kDa, Complex)
- Nucleosome (Complex from Xenopus laevis)
- Chromodomain-helicase-dna containing protein (Macromolecule)
- Chd1 (Complex from Saccharomyces cerevisiae)
Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes.
Sundaramoorthy R, Hughes AL, Singh V, Wiechens N, Ryan DP, El-Mkami H, Petoukhov M, Svergun DI, Treutlein B, Quack S, Fischer M, Michaelis J, Bottcher B, Norman DG, Owen-Hughes T
eLife (2017) 6 [ Pubmed: 28332978 DOI: doi:10.7554/eLife.22510 ]
- Chd1 (Complex from Saccharomyces cerevisiae)
- Chd1-nucleosome complex (400 kDa, Complex)
- Chromodomain helicase dna binding protein (Macromolecule)
- Nucleosome (Complex from Xenopus laevis)