TBOA-bound GltPh RSMR mutant in IFS state
Resolution: 2.55 Å
EM Method: Single-particle
Fitted PDBs: 7ug0
Q-score: 0.596
Huang Y, Reddy KD, Bracken C, Qiu B, Zhan W, Eliezer D, Boudker O
J Am Chem Soc (2023) 145 pp. 8583-8592 [ DOI: doi:10.1021/jacs.3c01003 ]
- (3s)-3-(benzyloxy)-l-aspartic acid (239 Da, Ligand)
- Water (18 Da, Ligand)
- Glutamate transporter homolog (44 kDa, Protein from Pyrococcus horikoshii)
- Sodium ion (22 Da, Ligand)
- Rsmr mutant bound with na/tboa (44 MDa, Cellular component from Pyrococcus horikoshii)
Asp-bound GltPh RSMR mutant in IFS-A1 state
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 7ugd
Q-score: 0.534
Huang Y, Reddy KD, Bracken C, Qiu B, Zhan W, Eliezer D, Boudker O
J Am Chem Soc (2023) 145 pp. 8583-8592 [ DOI: doi:10.1021/jacs.3c01003 ]
- Aspartic acid (133 Da, Ligand)
- Glutamate transporter homolog (44 kDa, Protein from Pyrococcus horikoshii)
- Gltph (Complex from Pyrococcus horikoshii)
- Sodium ion (22 Da, Ligand)
TBOA-bound GltPh RSMR mutant in OFS state
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 7ugj
Q-score: 0.531
Huang Y, Reddy KD, Bracken C, Qiu B, Zhan W, Eliezer D, Boudker O
J Am Chem Soc (2023) 145 pp. 8583-8592 [ DOI: doi:10.1021/jacs.3c01003 ]
- (3s)-3-(benzyloxy)-l-aspartic acid (239 Da, Ligand)
- Tboa-bound gltph rsmr mutant in ofs state (Complex from Pyrococcus horikoshii)
- Sodium ion (22 Da, Ligand)
- Glutamate transporter homolog (44 kDa, Protein from Pyrococcus horikoshii)
Asp-bound GltPh RSMR mutant in IFS-A2 state
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 7ugv
Q-score: 0.532
Huang Y, Reddy KD, Bracken C, Qiu B, Zhan W, Eliezer D, Boudker O
J Am Chem Soc (2023) 145 pp. 8583-8592 [ Pubmed: 37023263 DOI: doi:10.1021/jacs.3c01003 ]
- Aspartic acid (133 Da, Ligand)
- Glutamate transporter homolog (44 kDa, Protein from Pyrococcus horikoshii)
- Gltph (Complex from Pyrococcus horikoshii)
- Sodium ion (22 Da, Ligand)
Asp-bound GltPh RSMR mutant in IFS-B1 state
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 7ugx
Q-score: 0.499
Huang Y, Reddy KD, Bracken C, Qiu B, Zhan W, Eliezer D, Boudker O
J Am Chem Soc (2023) 145 pp. 8583-8592 [ Pubmed: 37023263 DOI: doi:10.1021/jacs.3c01003 ]
- Aspartic acid (133 Da, Ligand)
- Glutamate transporter homolog (44 kDa, Protein from Pyrococcus horikoshii)
- Gltph rsmr mutant bound with na and asp (Complex from Pyrococcus horikoshii)
- Sodium ion (22 Da, Ligand)
Asp-bound GltPh RSMR mutant in iOFS state
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 7uh3
Q-score: 0.463
Huang Y, Reddy KD, Bracken C, Qiu B, Zhan W, Eliezer D, Boudker O
J Am Chem Soc (2023) 145 pp. 8583-8592 [ DOI: doi:10.1021/jacs.3c01003 ]
- Aspartic acid (133 Da, Ligand)
- Glutamate transporter homolog (44 kDa, Protein from Pyrococcus horikoshii)
- Gltph rsmr mutant bound with na and asp (Complex from Pyrococcus horikoshii)
- Sodium ion (22 Da, Ligand)
Asp-bound GltPh RSMR mutant in IFS-B2 state
Resolution: 3.44 Å
EM Method: Single-particle
Fitted PDBs: 7uh6
Q-score: 0.402
Huang Y, Reddy KD, Bracken C, Qiu B, Zhan W, Eliezer D, Boudker O
J Am Chem Soc (2023) 145 pp. 8583-8592 [ DOI: doi:10.1021/jacs.3c01003 ]
- Aspartic acid (133 Da, Ligand)
- Glutamate transporter homolog (44 kDa, Protein from Pyrococcus horikoshii)
- Gltph rsmr mutant bound with na and asp (Complex from Pyrococcus horikoshii)
- Sodium ion (22 Da, Ligand)
GltPh in complex with L-aspartate and sodium ions in outward-facing state
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 6uwf
Q-score: 0.553
Huang Y, Wang X, Lv G, Razavi AM, Huysmans GHM, Weinstein H, Bracken C, Eliezer D, Boudker O
Nat Chem Biol (2020) 16 pp. 1006-1012 [ DOI: doi:10.1038/s41589-020-0561-6 Pubmed: 32514183 ]
- Aspartic acid (133 Da, Ligand)
- Glutamate transporter homolog (44 kDa, Protein from Pyrococcus horikoshii)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Complex of gltph with l-aspartate and sodium ions in msp1e3 nanodisc (134 kDa, Complex from Pyrococcus horikoshii)
GltPh in complex with L-aspartate and sodium ions in intermediate outward-facing state
Resolution: 3.62 Å
EM Method: Single-particle
Fitted PDBs: 6uwl
Q-score: 0.454
Huang Y, Wang X, Lv G, Razavi AM, Huysmans GHM, Weinstein H, Bracken C, Eliezer D, Boudker O
Nat Chem Biol (2020) 16 pp. 1006-1012 [ DOI: doi:10.1038/s41589-020-0561-6 Pubmed: 32514183 ]
- Aspartic acid (133 Da, Ligand)
- Glutamate transporter homolog (44 kDa, Protein from Pyrococcus horikoshii)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Complex of gltph with l-aspartate and sodium ions in msp1e3 nanodisc (134 kDa, Complex from Pyrococcus horikoshii)