MVV strand transfer complex (STC) intasome in complex with LEDGF/p75 at 3.5 A resolution
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7z1z
Q-score: 0.19
Ballandras-Colas A, Chivukula V, Gruszka DT, Shan Z, Singh PK, Pye VE, McLean RK, Bedwell GJ, Li W, Nans A, Cook NJ, Fadel HJ, Poeschla EM, Griffiths DJ, Vargas J, Taylor IA, Lyumkis D, Yardimci H, Engelman AN, Cherepanov P
Nat Commun (2022) 13 pp. 2416- [ DOI: doi:10.1038/s41467-022-29928-8 ]
- Mvv stc intasome in complex with ledgf (480 kDa, Complex from Visna/maedi virus EV1 KV1772)
- Zinc ion (65 Da, Ligand)
- Pc4 and sfrs1-interacting protein (11 kDa, Protein from Homo sapiens)
- Dna (5'-d(*gp*cp*tp*gp*cp*gp*ap*gp*ap*tp*cp*cp*gp*cp*tp*cp*cp*gp*gp*tp*g)-3') (7 kDa, DNA from DNA molecule)
- Pol polyprotein (32 kDa, Protein from Visna/maedi virus EV1 KV1772)
- Dna (5'-d(p*tp*tp*gp*ap*tp*tp*ap*gp*gp*gp*tp*g)-3') (7 kDa, DNA from DNA molecule)
- Dna (37-mer) (15 kDa, DNA from DNA molecule)
MVV cleaved synaptic complex (CSC) intasome at 4.5 A resolution (re-refined)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 7zpp
Q-score: 0.097
Ballandras-Colas A, Maskell DP, Serrao E, Locke J, Swuec P, Jonsson SR, Kotecha A, Cook NJ, Pye VE, Taylor IA, Andresdottir V, Engelman AN, Costa A, Cherepanov P
Science (2017) 355 pp. 93-95 [ Pubmed: 28059770 DOI: doi:10.1126/science.aah7002 ]
- Vdna, non-transferred strand (6 kDa, DNA from Visna lentivirus (strain 1514))
- Vdna, transferred strand (5 kDa, DNA from Visna-maedi virus)
- Integrase (32 kDa, Protein from Visna/maedi virus EV1 KV1772)
- Vdna (Complex from Visna-maedi virus)
- Maedi-visna virus (mvv) intasome (542 kDa, Complex)
- Intergrase (Complex from Visna/maedi virus EV1 KV1772)
MVV cleaved synaptic complex (CSC) intasome at 3.4 A resolution
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 7u32
Q-score: 0.256
Ballandras-Colas A, Chivukula V, Gruszka DT, Shan Z, Singh PK, Pye VE, McLean RK, Bedwell GJ, Li W, Nans A, Cook NJ, Fadel HJ, Poeschla EM, Griffiths DJ, Vargas J, Taylor IA, Lyumkis D, Yardimci H, Engelman AN, Cherepanov P
Nat Commun (2022) 13 pp. 2416-2416 [ Pubmed: 35504909 DOI: doi:10.1038/s41467-022-29928-8 ]
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Dna ev273 (8 kDa, DNA from synthetic construct)
- Dna ev272 (8 kDa, DNA from synthetic construct)
- Mvv cleaved synaptic complex intasome (480 kDa, Complex from Streptomyces griseus)
- Integrase (32 kDa, Protein from Visna/maedi virus EV1 KV1772)
Cryo-EM reconstruction of the maedi-visna virus (MVV) strand transfer complex
Resolution: 8.2 Å
EM Method: Single-particle
Fitted PDBs: 5m0r
Q-score: 0.098
Ballandras-Colas A, Maskell DP, Serrao E, Locke J, Swuec P, Jonsson SR, Kotecha A, Cook NJ, Pye VE, Taylor IA, Andresdottir V, Engelman AN, Costa A, Cherepanov P
Science (2017) 355 pp. 93-95 [ Pubmed: 28059770 DOI: doi:10.1126/science.aah7002 ]
- Nucleic acid (Complex from Visna lentivirus (strain 1514))
- Tdna (7 kDa, DNA from synthetic construct)
- Integrase (32 kDa, Protein from Maedi visna virus (strain KV1772))
- Vdna-tdna, transferred strand, joined to a model tdna (15 kDa, DNA from Visna lentivirus (strain 1514))
- Mvv strand transfer complex (Complex)
- Intergrase (Complex from Maedi visna virus (strain KV1772))
- Vdna, non-transfered strand (6 kDa, DNA from Visna lentivirus (strain 1514))
- Nucleic acid (Complex from synthetic construct)