Park EN, Mackens-Kiani T, Berhane R, Esser H, Erdenebat C, Burroughs AM, Berninghausen O, Aravind L, Beckmann R, Green R, Buskirk AR
EMBO J (2024) 43 pp. 484-506 [ DOI: doi:10.1038/s44318-023-00010-3 Pubmed: 38177497 ]
Cryo-EM reconstruction of Bacillus subtilis collided disome binding MutS2 SMR and KOW domains
Park EN, Mackens-Kiani T, Berhane R, Esser H, Erdenebat C, Burroughs AM, Berninghausen O, Aravind L, Beckmann R, Green R, Buskirk AR
EMBO J (2024) 43 pp. 484-506 [ DOI: doi:10.1038/s44318-023-00010-3 Pubmed: 38177497 ]
Bacillus subtilis MutS2-collided disome complex (stalled 70S)
Resolution: 3.57 Å
EM Method: Single-particle
Fitted PDBs: 8r55
Q-score: 0.386
Park EN, Mackens-Kiani T, Berhane R, Esser H, Erdenebat C, Burroughs AM, Berninghausen O, Aravind L, Beckmann R, Green R, Buskirk AR
EMBO J (2024) 43 pp. 484-506 [ Pubmed: 38177497 DOI: doi:10.1038/s44318-023-00010-3 ]
- 16s rrna (1533-mer) (496 kDa, RNA from Bacillus subtilis)
- 30s ribosomal protein s13 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s12 (15 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s11 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s9 (14 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s7 (17 kDa, Protein from Bacillus subtilis)
- Mrna (33-mer) (10 kDa, RNA from Bacillus subtilis)
- 50s ribosomal protein l15 (15 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Bacillus subtilis)
- B. subtilis endonuclease muts2 binding the collided ribosome interface (Complex from Bacillus subtilis)
- Large ribosomal subunit protein bl33 (5 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s16 (10 kDa, Protein from Bacillus subtilis)
- 5s rrna (112-mer) (36 kDa, RNA from Bacillus subtilis)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l36 (4 kDa, Protein from Bacillus subtilis)
- Trna (77-mer) (24 kDa, RNA from Bacillus subtilis)
- 30s ribosomal protein s10 (11 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul24 (10 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s19 (10 kDa, Protein from Bacillus subtilis)
- Muts2-disome complex from b. subtilis, focus refined, collided 70s (Complex from Bacillus subtilis)
- 30s ribosomal protein s3 (24 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s8 (14 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl31 (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s2 (28 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Bacillus subtilis)
- 23s rna (2887-mer) (949 kDa, RNA from Bacillus subtilis)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl28 (6 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul23 (10 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s17 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s15 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s4 (22 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s6 (11 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul6 (19 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l19 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s20 (9 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl27 (8 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l14 (13 kDa, Protein from Bacillus subtilis)
- Endonuclease muts2 (87 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l18 (12 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s5 (17 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Bacillus subtilis)
- Trna (73-mer) (23 kDa, RNA from Bacillus subtilis)
- Large ribosomal subunit protein bl17 (13 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l32 (6 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul2 (30 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s18 (8 kDa, Protein from Bacillus subtilis)
Bacillus subtilis MutS2-collided disome complex (stalled 70S)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8qpp
Q-score: 0.415
Park EN, Mackens-Kiani T, Berhane R, Esser H, Erdenebat C, Burroughs AM, Berninghausen O, Aravind L, Beckmann R, Green R, Buskirk AR
EMBO J (2024) 43 pp. 484-506 [ DOI: doi:10.1038/s44318-023-00010-3 Pubmed: 38177497 ]
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Bacillus subtilis)
- Trna (77-mer) (24 kDa, RNA from Bacillus subtilis)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl33 (5 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s12 (15 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s13 (13 kDa, Protein from Bacillus subtilis)
- Mrna (33-mer) (10 kDa, RNA from Bacillus subtilis)
- 30s ribosomal protein s20 (9 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul2 (30 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s15 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s17 (10 kDa, Protein from Bacillus subtilis)
- 16s rrna (1533-mer) (496 kDa, RNA from Bacillus subtilis)
- 30s ribosomal protein s5 (17 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s4 (22 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul24 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Bacillus subtilis)
- Muts2-disome complex from b. subtilis, focus refined, stalled 70s (Complex from Bacillus subtilis)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l18 (12 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l36 (4 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul6 (19 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl27 (8 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s18 (8 kDa, Protein from Bacillus subtilis)
- Endonuclease muts2 (87 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s3 (24 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl28 (6 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s16 (10 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s8 (14 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l14 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s6 (11 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl17 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl31 (7 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l15 (15 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l32 (6 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s7 (17 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l9 (16 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s2 (28 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s11 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul23 (10 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s19 (10 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l19 (13 kDa, Protein from Bacillus subtilis)
- 23s ribosomal rna (949 kDa, RNA from Bacillus subtilis)
- 30s ribosomal protein s9 (14 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Bacillus subtilis)
- 5s rrna (112-mer) (36 kDa, RNA from Bacillus subtilis)
- 30s ribosomal protein s10 (11 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Bacillus subtilis)